Tetra ARMS PCR: what it is and how it works
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- Опубликовано: 1 окт 2020
- Tetra ARMS PCR for mutation or SNP detection.
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Links to related videos:
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3. SSCP: • Single Strand Conforma...
4. Difference between SNP and Mutation
5. Markers and types: • Markers (Molecular/Gen...
6. ISSR (Inter simple sequence repeat): • ISSR (Inter Simple Seq...
7. RAPD: • RAPD Marker characteri...
8. RFLP (Restriction fragment length polymorphism): • RFLP marker
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Related reading:
• Alyethodi et al. 2018. T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
• Ahlawat et al. 2014 Designing, optimization and validation of tetra-primer ARMS PCR protocol for genotyping mutations in caprine Fec genes
• primer1.soton.ac.uk/primer1.html
• cedar.genetics.soton.ac.uk
This video really help me to understand the ARMS PCR, thank you so much.
Tanks. Stay connected for more.
This is an absolutely brilliant explanation. Thank you!
Thank you for the nice and informative vedioe. As I am working on it currently. One important point to mention is a mismatch on the 3rd prime end of the primer is intentionally put for the additional primer specificity
Thank you sooo much for this video sir🙏
Thank you so much, This video is very helpful !!!!
excellent
I have created an arms primer; might I perhaps email it for revision?
by designing ARMS PCR Primer, the primer sequence not complementary to the gene sequence, why
Gracias.
Sir, what is the difference between it and Allele specific PCR (AS-PCR) ?
nice video, do you recommend putting all of the four primers in one PCR? Thanks
Exactly.
Thank you 🙂✌
Happy to make these videos..
@@XploreBio I appreciate your hard work Sir, thanks a lot... !!!
Sequencing of DNA by Sanger...plzzz make video on this topic sir 🙏🏻
how do you make inner primer is with the same sequence ?
Sequence will be same. Just the region within that sequence will be different.
So nice
Thanks
How can we design if there is deletion or frameshift
@Shah. Need more details to answer.
How to desing primers for tetra arms pcr
There is an logiciel named primer 1
Detail video on tilling approch
Linkage disequilibrium
I want it video ppt or another file
email me at XploreBio@yahoo.com
@@XploreBio I sent for u
Good. I wait for love from you 💝💖
Keep watching..