Tetra ARMS PCR: what it is and how it works

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  • Опубликовано: 1 окт 2020
  • Tetra ARMS PCR for mutation or SNP detection.
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    Links to related videos:
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    4. Difference between SNP and Mutation
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    8. RFLP (Restriction fragment length polymorphism): • RFLP marker
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    Related reading:
    • Alyethodi et al. 2018. T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    • Ahlawat et al. 2014 Designing, optimization and validation of tetra-primer ARMS PCR protocol for genotyping mutations in caprine Fec genes
    • primer1.soton.ac.uk/primer1.html
    • cedar.genetics.soton.ac.uk

Комментарии • 32

  • @priscilapena9260
    @priscilapena9260 3 года назад +6

    This video really help me to understand the ARMS PCR, thank you so much.

    • @XploreBio
      @XploreBio  3 года назад

      Tanks. Stay connected for more.

  • @Plasmodium2002
    @Plasmodium2002 2 года назад +1

    This is an absolutely brilliant explanation. Thank you!

  • @muhammadawais2999
    @muhammadawais2999 Год назад

    Thank you for the nice and informative vedioe. As I am working on it currently. One important point to mention is a mismatch on the 3rd prime end of the primer is intentionally put for the additional primer specificity

  • @SAGAR-ou6ok
    @SAGAR-ou6ok 3 года назад +1

    Thank you sooo much for this video sir🙏

  • @likemusic2555
    @likemusic2555 2 года назад

    Thank you so much, This video is very helpful !!!!

  • @RanaAhmed-ch1vz
    @RanaAhmed-ch1vz 2 года назад +2

    excellent

  • @MohammadMahmoud-ji1jc
    @MohammadMahmoud-ji1jc 2 месяца назад

    I have created an arms primer; might I perhaps email it for revision?

  • @MohammadMahmoud-ji1jc
    @MohammadMahmoud-ji1jc Месяц назад

    by designing ARMS PCR Primer, the primer sequence not complementary to the gene sequence, why

  • @angelmejiap
    @angelmejiap Год назад

    Gracias.

  • @hassanah253
    @hassanah253 7 месяцев назад

    Sir, what is the difference between it and Allele specific PCR (AS-PCR) ?

  • @NK8004
    @NK8004 3 года назад +1

    nice video, do you recommend putting all of the four primers in one PCR? Thanks

  • @MonikaShailesh777
    @MonikaShailesh777 3 года назад +1

    Thank you 🙂✌

    • @XploreBio
      @XploreBio  3 года назад +1

      Happy to make these videos..

    • @MonikaShailesh777
      @MonikaShailesh777 3 года назад

      @@XploreBio I appreciate your hard work Sir, thanks a lot... !!!

  • @nehabhardwaj3483
    @nehabhardwaj3483 3 года назад

    Sequencing of DNA by Sanger...plzzz make video on this topic sir 🙏🏻

  • @aylinadame4726
    @aylinadame4726 2 года назад +1

    how do you make inner primer is with the same sequence ?

    • @XploreBio
      @XploreBio  2 года назад

      Sequence will be same. Just the region within that sequence will be different.

  • @user-er4cl2zt6k
    @user-er4cl2zt6k 2 года назад

    So nice

  • @imrocknreeling
    @imrocknreeling 3 года назад

    How can we design if there is deletion or frameshift

    • @XploreBio
      @XploreBio  3 года назад

      @Shah. Need more details to answer.

  • @user-fi9tt6xo1g
    @user-fi9tt6xo1g 3 года назад +1

    How to desing primers for tetra arms pcr

  • @ROHIT-sm3bi
    @ROHIT-sm3bi 11 месяцев назад

    Detail video on tilling approch

  • @samarthkumar5198
    @samarthkumar5198 3 года назад

    Linkage disequilibrium

  • @user-er4cl2zt6k
    @user-er4cl2zt6k 2 года назад

    I want it video ppt or another file

  • @prj4550
    @prj4550 3 года назад

    Good. I wait for love from you 💝💖