Great video - very clear explanations. I just want to point out that for the new OCR Biology A H032 specification, the plots mustn't be joined up on the graph like in the video, although they had to be for the old specification. As there is a general trend, a smooth line/curve of best fit should be drawn through.
@stranger things the results beer to be comparable. Tissue from different beetroots may contain varying amounts of the red pigment so give different results. We need to coloured water pouring/decanting into colorimeter cuvettes.
Thanks for your comment. I use these little videos quite a lot with my classes. Before they do the practical lesson and for revision. Your correct about the pigment.
Cork borers are available from all scientific and educational suppliers. A chipper can be used instead as the y don't have to be cylindrical. Or just cut similar chips with a knife.
i am doing my prac on effect of Ph on the permeability of the cell. but i dont know how the pigment is going to leak. what does it do to the membranes. please help
Just for the absorbance of light in the beetroot liquid solution, but in my practical we used blue light. I don't really think it matters too much as long as it is the opposite colour as the beetroot solution (i.e. it's red so blue or green colours are opposite on the colour wheel
you're testing the permeability of the cell membrane of beetroot at varied temperatures, which therefore means that at different temperatures more or less pigment will move through the beetroot and 'leaks' out into the water in the boiling tube (i.e. at higher temperatures, more beetroot pigment is leaked out) at a set amount of time - say 10 minutes for all temperatures. You must decanter the beetroot solution into another boiling tube quickly to prevent further leakage that would disrupt the results. This solution is used to test the absorbance of light - depending on the concentration of the pigment leaked out, the light absorbed will be more or less, and you cannot just use qualitative data, i.e. just by looking at the concentration to determine the results. The colourimeter checks the absorbance of light in the liquid of the beetroot solution which gives quantitative data.
The betalain comes from the vacuole within the cells of the beetroot which also has a membrane surrounding it, and therefore the betalain has to pass through two membranes to move into the water in the boiling tube. It therefore doesn't really just show the permeability of the cell surface membrane as it also shows the permeability of the vacuole.
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POV: tomorrow you have your first official A level practical.
How did you know OMG?
POV u have mocks 💀
@@hallepholana4876 POV you have the actual A-level in two days
No it's my 4th 😂
@@vlsui66 POV you’re in your 2nd yr and you just found out the test in 2hrs is about a practical you did in 1st yr
Great video - very clear explanations. I just want to point out that for the new OCR Biology A H032 specification, the plots mustn't be joined up on the graph like in the video, although they had to be for the old specification. As there is a general trend, a smooth line/curve of best fit should be drawn through.
this is the only video i've found that has explained the practical clearly and precisely 👏🏽 thank you so much!!
Ur way of explanation is mind blowing and ur voice is also amazing thanks for this video
My g
This is incredible for revision of the practicals, extremely clear and concise thank you very much!
Control variables. Same volume of water. Cylinders cut to same size to ensure same surface area in contact with water. Also cut from same beetroot.
@stranger things the results beer to be comparable. Tissue from different beetroots may contain varying amounts of the red pigment so give different results. We need to coloured water pouring/decanting into colorimeter cuvettes.
The pigment is actually Betalain, but great video! I will be using this with my class :)
Thanks for your comment. I use these little videos quite a lot with my classes. Before they do the practical lesson and for revision. Your correct about the pigment.
Yes ,betalain especially betacyanin
Cork borers are available from all scientific and educational suppliers. A chipper can be used instead as the y don't have to be cylindrical. Or just cut similar chips with a knife.
Very clear explanation! I use the video to support my teaching. Thanks for the effort.
Cool video love this and the beets look fit as well
Jake andrew Thank you Jake.
now this is pog champ
Excellent video! Thank you so much!
Greatly informative video overall, however one small inaccuracy as the chemical source of the pigment is actually Betalain .
You have helped me so much, thank you.
just wondering how the ralationship between absorbance measured by colorimeter and colour intensity of the solution??
What are the standardized variable in this experiment? And why beetroot and not a carrot ?
why does the beet have to be in the water for 20 min?
Thank you madam I am a botony student it was really helpful for my main practicles... Really thank you🙏
What kinds of things do you study in botany and does it require you to go outdoors alot?
This is a great video. Thanks. Are there any limitations of this procedure?
Could you turn captions on please?
Does this video explain all that is given in edexcel IAL core practical 3( I mean the 3rd pactical ? )
Why is it important to leave the beetroots in the test tubes submerged in the respective water bath for 20 minutes? (i.e. why not longer or shorter?)
Im just gonna point out that almost EVERY PERSON HERE is a teacher
No..
This was really good thank you
only here cuz my teacher told me so
I’ve tried this in class but mine is not working I’m not sure what I’m missing
That was really useful, thank you!
This is very helpful, thank you!
how do you spell the thing that you put the solution in for the calorimeter
addie pat a cuvette.
Biology Practical Activities and Revision thank you
What is the benefit?
i was advised to ise red cabbageinsteadecause beetroot was used by another student. would that work the same?
It may work. I have never tried it. Good luck with that.
Very helpful. Thank you !
I found it Mrs Iancu :)
Great video .thank u .
what does it mean to have reaches equilibrium in the graph at the end when it levels off
its becomes and isotonic solution bc the conc of beta lain in the vacuole is the same as in the water
what would you suggest the risks assessments are
No real risks with the beetroot practical except use of a knife or scalpel to cut the cylinders.
Does it matter what colour the colorimeter is set to, and if so, why?
Most colours would work to an extent but green works best. Avoid red and blue as they are reflected by the purple pigment.
@@biologypracticalsandrevisi8066 Thank you!
What was the results?
Can I have the name of equipments?
What is the principle of this test
what happen if we don't rinse beet root??
Good video
how the temperature affects membrane permeability??
im only here because my teacher wants me here
very useful thanks
Is a colorimeter and a spectrophotometer the same device?
No. There are some similarities but quite different.
For the graph - Do you join the points up with a ruler?
Kelsie M no you just do it by hand and hope it’s straight 🤔
@@AnimatedBlast You are actually not suppose to join the points BTW. its a line of best fit. so your dumb comment didnt make sense HLOG
do you need a control tube? what would that be..
No control needed
Why do we use green light
Green is complementary to the red/pink colour
@@jessicamcmullen7393 thx
Is there any assumption?
Thank you
i am doing my prac on effect of Ph on the permeability of the cell. but i dont know how the pigment is going to leak. what does it do to the membranes. please help
Laila Yaqoobi membrane proteins will be denatured by Ph much above or below 7 so the membrane will fall apart and become much more permeable.
Thank you for your help🙂🙂
Hello quarantined folks
thank you for the help
Why green light?
Just for the absorbance of light in the beetroot liquid solution, but in my practical we used blue light. I don't really think it matters too much as long as it is the opposite colour as the beetroot solution (i.e. it's red so blue or green colours are opposite on the colour wheel
why do you keep the beetroot in water?
you're testing the permeability of the cell membrane of beetroot at varied temperatures, which therefore means that at different temperatures more or less pigment will move through the beetroot and 'leaks' out into the water in the boiling tube (i.e. at higher temperatures, more beetroot pigment is leaked out) at a set amount of time - say 10 minutes for all temperatures. You must decanter the beetroot solution into another boiling tube quickly to prevent further leakage that would disrupt the results. This solution is used to test the absorbance of light - depending on the concentration of the pigment leaked out, the light absorbed will be more or less, and you cannot just use qualitative data, i.e. just by looking at the concentration to determine the results. The colourimeter checks the absorbance of light in the liquid of the beetroot solution which gives quantitative data.
Very Nice!!
this practical was in my exam
I have mine tomorrow😭😂
would you say this is an example of diffusion?
Sea Mitchell indeed it is.
and im only watching this now
It ws very helpful👍
Issrar is a beetroot chip
Thanks
Amazing
MHS anyone 2020 prac
Can u give error in the experiment
The betalain comes from the vacuole within the cells of the beetroot which also has a membrane surrounding it, and therefore the betalain has to pass through two membranes to move into the water in the boiling tube. It therefore doesn't really just show the permeability of the cell surface membrane as it also shows the permeability of the vacuole.
Love it
Good
We were testing alcohol
Emerge that’s a good one. It dissolves the lipid bilayer.
useful
wash your beetroot bestie
what can be used instead of beetroot?
misheel altan I have never tried it with anything else but it may work with red cabbage.
fab
my cro
Why do we use green light
The red blue pigment will absorb green light well. It would reflect red or blue light.
@@biologypracticalsandrevisi8066 why not yellow
You could use yellow but green works best.