How to Culture Isochrysis Phytoplankton
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- Опубликовано: 30 ноя 2024
- In this video I show you how to culture Isochrysis Phytoplankton. I go in full detail, step by step on how I culture it. Iso is very sensitive to light and is easily contaminated. There are special requirements needed to successfully culture it. If you have any questions or comments please let me know in the comment section below.
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Products used or mentioned in this video:
Isochrysis Starter Culture: www.ebay.com/i...
Amscope Microscope: amzn.to/41abWSP
Swift Microscope: amzn.to/40TGNn0
Pipettes: amzn.to/3KnITEx
500ml Erlenmeyer Flask: amzn.to/3moS8MD
LED Light Panel: amzn.to/41achVB
Grow Tent: amzn.to/43gNUra
Rigid Airline Tubing: amzn.to/40SyIip
Plastic Bottles: amzn.to/40QQMcx
Autoclave: amzn.to/43fw1ZQ
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• Copepods & Phytoplankton
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Is a home brewer, and reefer - I soak my one gallon phyto glass jugs in p.b.w. cleaner for about 20 min to clean, rinse and then use a spray bottle with SanStar and RODI water to sanitize and then rinse with RODI water through a UV so the SanStar does not drop the pH. Fast and works great. Great Video!
Thanks for your help.
Yes these tents and lamps are well know for being ideal for house plants, we use them here in the Netherlands too 😂..
Whats this plankton about? Food, problem solving?
@@randomdutchguy lol
It’s because you probably used F2 without silicates. It requires silicates. There’s a slew of other things but also, it looks like Teteaselmis as well got in there with Nanno.
Hi, iam have problems with protozoa, do you know how to clean the culture?
I do not. I'm sorry. I would just start a new culture..
phytoplankton food cultivation is possible to prepare in home?
For aquarium food, yes. It's very easy to culture it at home. For humans. I do not know anything about that.
@@BriansAquariums not human use ,phytoplankton fertilizer how to prepare?
@@Marine_karaikudi you should also be able to buy it from your local fish store or order it online.
Hi, mi phytoplankton mix culture became entirely golden-yellow after 5 days, now I have 2L Isochrisis, so I’m really happy. But how can I know the density to be ready to harvest?
@SamuelMENDOZAGARCIA You can look under a microscope. There's really no amount of density that makes a culture ready. It's up to you to decide how dence you would like it. You don't have to have the darkest phytoplankton in the world. LOL. Im not sure why people get caught up in that.
hey man, I am wanting to start my first nano phyto culture. is it ok to start off with plastic containers? I got them from amazon. I liked them because they have a spout already. I have most of the items needed including F/2 and the same pipettes. I also have a light that should work. I am wanting to do 2gal at a time and have 20 16oz sterile platic bottles to keep it after it's done.
Yes, it is perfectly fine to use plastic to culture in.
@@BriansAquariums cool I started that up Friday. Hope I get a good culture
Solid video, I have purchased Nanno from you in the past. Didn’t realize you sold ISO as well.
I do, but I have not been culturing as much lately.
How about Rotifers?
Good topic! I will work on that video.
Household plants huh??? 😎 lol, jk. Nice information.
Shhh! Those are special household plants.