Mechanism of Smooth Muscle Relaxation | Role of Nitric Oxide | IP3 Pathway

In this video the mechanism of Smooth muscle relaxation have been discussed in detail.
The smooth muscle surrounds the endothelium tissue which produces the signalling molecules like Nitric oxide.
The Nitric oxide is produced via G-Protein cascade which involves IP3 secondary messenger.
The following have been discussed in the video :
1.Mechanism of Smooth muscle relaxation
2.Role of Nitric Oxide in Vasodilation
3.Structure of Blood vessel ( brief )
4.IP3 Inositol Pathway.
5.Calmodulin Activation.

[@hussainbiology]

Read MLCP instead of MLCK @ 4:51
Myosin Light Chain Phosphatase.

[@SofiaBorbolet]

Thanks I liked this vídeo a lot

[@hussainbiology]

@@SofiaBorbolet You are welcome..✌️✌️

[@MercyMburuNyakio]

Calmodulin is a CALcium-MODULated protein. Hussain, you have helped my being on this earth exceptionally. When you said this, I felt it. Thank you! Thank you! Thank you!

[@hussainbiology]

thanks Mercy for appreciation..Glad to know that it helps ✌️

[@mohammad.shokrolahi]

saved me from reading 10 to 20 pages , thanks

[@hussainbiology]

Really Glad to know that it helps....

[@naileusmile2293]

Using 5mins to explain 2hours of lecture. Thank you!!!!!!!

[@hussainbiology]

Thanks Alice again for appreciating my work......My motto is always to put forward these hour lectures in just few minutes...... everything has gone fast why not studying

[@neerudugauthami9246]

You were super fast yet made it simple and interesting.

[@Insaki1]

Amazing video, really helped me in understanding how eNos become NO

[@Warona111]

amazing talk. thank you very much. I was making a summary of two papers i was reading; a review paper on physiological role of NO and one on thyroid hormones and L-arginine transport.

[@soco2020]

Amazing explanation. Thank you

[@Bee-rw4vh]

What a brilliant explanation. For real! Thank you so much!

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@alifadila3364]

You my friend are amazing! This is exactly what I was looking for!

[@hussainbiology]

Thanks Ali for appreciation....

[@ughsirius]

Thank you so much!!

[@hussainbiology]

most Welcome Aiza

[@kalvinryanbrookes3551]

Hello Hussein, thank you for this video.

[@hussainbiology]

Thanks Kalvin for appreciation...Glad to know that it helps

[@soeminwaiyan2734]

College lecturer: 45 min lecture
RUclips video: 5 min is enough mate

[@yogeshlakshman8488]

Thank you
Great presentation sir

[@sanaafreen1937]

The best video on this topic

[@hussainbiology]

Thanks.....I really appreciate ur words...... This induces more confidence

[@sanaafreen1937]

You deserve it. Waiting for more Human Physiology videos.

[@hussainbiology]

Thanks Again, i would have posted more Physiology videos but i thought there are too many better options existing here in RUclips,,,,,,,

[@hussainbiology]

So where from you are ? and are you Miss or Mr ?

[@sanaafreen1937]

Indian. Miss. Can you link some good physiology sites?

[@pratibhathakur1986]

Well explained ....thanks sir

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@muhammadbilalsarwar1711]

amazing explanation 🎉

[@MegaJeves]

Hello. Can you explain the role of nitrates in the nitric oxide chain? I am wondering if an increase in consumption of foods high in nitrates helps the body’s total production of NO when supplemented with L Citrulline before a workout. Thanks!

[@mattjackson1149]

really helpful, thanks

[@hussainbiology]

thanks for appreciation ✌️Glad to know that it helps ✌️

[@ahmetkeyan3346]

thank you bro i love india

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@kajalmemane2842]

thank you...its really helpful..great job sir.

[@hussainbiology]

Thanks Kajal for appreciation......& i ain't a sir yet......

[@aamina5119]

Thank you immensely 🙌🏽

[@hussainbiology]

thanks for appreciation....Glad to know that it helps

[@ramchandrasuthar2848]

Great analysis sir

[@hussainbiology]

Thanks Ramchandra for ur support

[@step_zero]

great video

[@hussainbiology]

Thanks......appreciate ur support

[@shweta6599]

You are amazing bro. Thank you so much 😊

[@hussainbiology]

thanks for appreciation

[@damiettes7140]

So will dietary NO like from beets help relax involuntary smooth muscle contractions? This happens a lot in people with the rare disease of PNH - Paroxysmal Nocturnal Hemoglobinuria.
They are in pain when this happens. Trying to find out if dietary NO has good bioavailability so it will relax their pain

[@keepitsimple3042]

Love it

[@ashirt2100]

MLCK is a kinase .A kinase always transfers phosphate groups to its substrates.I am a bit confused .plz help.

[@hussainbiology]

Apologies first , this is the editing mistake i have made here. Instead of MLCP i have displayed MLCK but i have said Myosin Light Chain Phosphatase.
So plz read MLCP instead of MKCK.
Sorry again

[@ashirt2100]

@@hussainbiology Thank you.Great job!!!

[@hussainbiology]

@@ashirt2100 You are welcome... BTW thanks for highlighting this error.

[@sameepthakuri9550]

Why is the phosphatase called myosin light chain KINASE ? Arent the kinase and phosphatase’s role opposite to each other ?

[@hussainbiology]

Apologies first , this is the editing mistake i have made here. Instead of MLCP i have displayed MLCK but i have said Myosin Light Chain Phosphatase.
So plz read MLCP instead of MKCK.
Sorry again

[@sameepthakuri9550]

Hussain Biology thanks bro. I spent 3 hours stuck on this stupid cycle !

[@JC-rv6iq]

I have a question. Since calcium calmodulin complex promotes muscle contraction as it activates myosin light chain kinase and phosphorylation of myosin light chain will cause the contraction of smooth muscle, But on the other hand, calcium calmodulin complex seems to again cause vasodilation, how does it suppose to bring response?

[@Piz_lycanz]

thank you for the vid... just wondering, isnt the function of calmodulin is for muscle contraction? does it mean it will cause both contraction and relaxation? and also cGMP will stimulate MLCK or MLCP? Thank you for the help

[@vikas1933]

nice explanation. can you please show us the summary of all the explanation (illustration ) in the last it would be better for just revising

[@hussainbiology]

Thanks....In the future videos i will keep this thing in mind...

[@jiyaali3664]

👍👌

[@hussainbiology]

thanks for appreciation.. Glad to know that it helps

[@AliHassan-kn9it]

can smooth muscle cells regenerate by itself and how many days need to regenerate?

[@venkatesanmunusamy7319]

THANKS

[@hussainbiology]

Glad to know that it helps.....

[@joshholymoses]

Gotta keep you on 0.5 speed my friend

[@zainuddin1403]

Is calmodulin also present in endothelial cells?

[@patriceparham9517]

I was recommended a preworkout product called 'Noxygen' for my chronic muscular pain/numbness. At first, I didn't understand this recommendation. I completely understand now.
Vasodilation will increase blood flow to my muscles, which will help carry out toxins (lactic acid), and deliver nutrients.
Is this correct?

[@hussainbiology]

Yeah that will occur more when u take products like that......Vasodilation will be more pronounced after taking Noxygen...... Noxygen indirectly boosts the production of Nitric Oxide within the blood vessels.....

[@ahmqun8909]

hell yea

[@kardonay]

I realise this is old but I hope someone can help me out. I have Sphincter of Odds Dysfunction type 3 - My symptoms reduced a lot after a sphincterecomy but I still have attacks a couple of times a year. I have tried GTN with no success - Should I try the nitrous oxide inhalation (like the kids do for funsies) next time I have an attack? Normally I end up in ED getting hard core drugs to settle it (which don't always work) & would like to avoid it as much as possible. Any input would be greatly appreciated.

[@delestonbrantley5274]

Hussain this was very informative and helpful. Can you please list some sources you used for this video that can help me in my research. Thank you.

[@hussainbiology]

1.Molecular Biology of Cell by Alberts ( In Mechanisms of Cell Communication Chapter )
2.NCBI website

[@loreleihennessy3034]

Hi - Awesome video, thanks. Just one thing, could you please clarify what happens to DAG? I slowed the video down but just could not quite catch what you said. Cheers

[@hussainbiology]

The PIP2 is attached into the plasma membrane and is acted upon by Phospholipase C which cleaves PIP 2 DAG and IP3......DAG remains attached to Plasma Membrane while as Ip3 is released. The Attached DAG then interacts with other molecules like Protein Kinase C.
To see what happens to DAG and IP3 do watch this video also , it will consume only 3 mins
ruclips.net/video/-5hNTIVk7IE/видео.html

[@dkumar4024]

where contraction occur? no clear views after releasing and binding of Ca++ with Calmodulin what happen next and how it reach to contration mechanism. what happen to myosin and actin and how they make cycling of the myosin actin cross bridges?........I think you explain only half picture....

[@ginjeti]

Is nitric oxide only synthesized and released by endothelial cells and into the smooth muscle ? Or do smooth muscle also have a storage of its own NO ? Thanks

[@hussainbiology]

As of now no NOS ( Nitric oxide synthase ) has been found in Smooth muscle.
NO synthesized by NOS3 in the endothelium has been considered to be the major source of NO for regulating vasoactivity.
NO could also be synthesized by mitochondrial nitric oxide synthase (mtNOS), but the existence of mtNOS is still under debate
Now there were some claims that Myoglobin + Nitrate in the Smooth muscle can produce NO, but the enzymes are still not known and the sources for the Myoglobin + Nitrate into NO is still in nascent stage.
Thanks

[@hussainbiology]

www.ncbi.nlm.nih.gov/pmc/articles/PMC2932548/figure/f2/
this will give u the whole idea.
Since NO is produced in the nervous tissue also.

[@Dazzletoad]

Can you please produce something regarding neurogenic vasoconstriction.

[@LaloboJDavid]

so beautiful .refference books please

[@halimearslanlar7841]

Can you explain the mechanisms by which an increase in cytosolic Ca2+ concentration initiates contractile activity in skeletal muscle cells please

[@david_4246]

Who discovered all this and how?

[@user-hk9gn7tr6r]

What causes relaxation of bronchial smooth muscle? A_ leukotrienes B_vasoactive intestinal polypeptide C_sulfur dioxide D_acetylcholine E_cool air Please help me i have a project and i can’t find the answer to this!

[@hussainbiology]

Your Comment had gone into Spam folder.
BTW the answer should be B ( Vasoactive intestinal Polypeptide)

[@henarafiq9952]

I can tell by your accent you are kashmiri......Nice video tho 👍

[@hussainbiology]

Yes..Indeed i am Kashmiri 😊✌️

[@sarasims2207]

T H A N K Y O U

[@hussainbiology]

Thanks Sara for appreciation...