How do you get rid of the halos in the result, the one that you got in the end? I work in similar fashion everytime, but sometime I get good dots , but sometimes I end up with halos. What do you use for blocking?1% BSA when poured on a well without the antigen, yields signal when treated with primary and secondary antibody followed by the ECL mix that I use in my lab. What would you suggest to use for blocking other tahn BSA?
In the manual for the Bio-Dot apparatus it says halos are the result of the membrane not being properly rehydrated before applying samples OR excessive concentrations of sample being loaded
+Ruth Angelina Not in the incubation steps (antigen, blockign solution, primary and secondary antibodies). The video is not very clear on that, but they have a detailed protocol on their webpage. sydney.edu.au/medicine/bosch/facilities/molecular-biology/protein-analysis/Bio_Dot_Instructure%20Manual_M1706545.pdf
the end result, however, looks very bad
Could someone explain what is going on?
How do you get rid of the halos in the result, the one that you got in the end? I work in similar fashion everytime, but sometime I get good dots , but sometimes I end up with halos.
What do you use for blocking?1% BSA when poured on a well without the antigen, yields signal when treated with primary and secondary antibody followed by the ECL mix that I use in my lab.
What would you suggest to use for blocking other tahn BSA?
In the manual for the Bio-Dot apparatus it says halos are the result of the membrane not being properly rehydrated before applying samples OR excessive concentrations of sample being loaded
the result is not optimal. Also, the key control in turning on or off the vacuum was omitted. Good video, but can be more helpful for sure.
what is this plastic thing filled with pores called ??? i meant the two bits of them
Steps on are on the bottom right.
is the vacuum running all the time?
+Ruth Angelina Not in the incubation steps (antigen, blockign solution, primary and secondary antibodies). The video is not very clear on that, but they have a detailed protocol on their webpage.
sydney.edu.au/medicine/bosch/facilities/molecular-biology/protein-analysis/Bio_Dot_Instructure%20Manual_M1706545.pdf
i have question, so if the color stronger it's mean the bind is stronger?
yes
Thanks So much
wht the hell is going on :/
xD
Sanjukta Dasgupta hiiiii
Nice
Niceeee