BD Bactec Turbid vial | Serratia| Blood Culture
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- Опубликовано: 19 окт 2024
- It appears you're referring to the context of using a BD Bactec system to identify Serratia species in a blood culture, specifically when the vial becomes turbid. Here are some key points:
BD Bactec System and Turbid Vial
BD Bactec System:
The BD Bactec system is used for detecting microbial growth in blood cultures.
It monitors changes in carbon dioxide levels produced by microbial metabolism, indicating growth.
Turbid Vial:
Turbidity in the vial suggests microbial growth.
A positive indication for the presence of bacteria or fungi.
Serratia in Blood Culture
Serratia spp.:
Serratia, especially Serratia marcescens, is a Gram-negative bacterium.
Known for causing nosocomial infections and for its resistance to multiple antibiotics.
Clinical Relevance:
Serratia can cause infections in immunocompromised patients.
Important to promptly identify and perform antibiotic susceptibility testing.
Procedure After Detecting Turbidity
Gram Stain:
Perform a Gram stain to confirm the presence of Gram-negative rods.
Subculture:
Subculture from the positive vial onto appropriate agar plates (e.g., MacConkey agar) to isolate the organism.
Identification:
Use biochemical tests, automated systems like VITEK, or MALDI-TOF MS for accurate identification.
Antibiotic Susceptibility Testing:
Essential for guiding appropriate therapy due to potential multidrug resistance.
Keywords for BD Bactec Turbid Vial, Serratia, and Blood Culture
BD Bactec
Turbid vial
Blood culture
Serratia marcescens
Gram-negative bacteria
Microbial growth
Positive culture
Antimicrobial resistance
Hospital-acquired infection
Automated detection
Incubation
Carbon dioxide levels
Gram stain
Subculture
MacConkey agar
Biochemical tests
VITEK
MALDI-TOF MS
Antibiotic susceptibility
Clinical significance
Immunocompromised patients
Pathogen identification
Infection control
Laboratory safety
Nosocomial infection