There's always a liquid inside my tip whenever I pipet. I make sure all the liquid has been dispensed when I press down to the full stop (by observing a bubble at the end of the tip). I think that the liquid that remains inside the tip (after I dispense the contents) is due to the liquid surrounding the outside of the tip that is close to the end of the tip. It enters the tip again when I release the plunger. Am I right?
I wish they taught this in my PhD. Had to figure this out on my own!
Best of luck! Hope this was helpful
The last one is key for blood. You really don't want to mix your plasma/serum by mistakenly pulling instead of pushing liquid.
Great tips as always!
Thanks!
There's always a liquid inside my tip whenever I pipet. I make sure all the liquid has been dispensed when I press down to the full stop (by observing a bubble at the end of the tip). I think that the liquid that remains inside the tip (after I dispense the contents) is due to the liquid surrounding the outside of the tip that is close to the end of the tip. It enters the tip again when I release the plunger. Am I right?
Likely. Be sure to keep your thumb down when you pull out. Good luck!