Practical Aspects of HPLC Method Development

[@49-wakchauresanket51]

Very nice video lec..more important and helpfull..thankyou 🎉

[@ojaswighadge7435]

Very very excellent Dr. Sonal. Thanks for such a useful piece of information.

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[@swarnalatha1451]

Excellent explanation thanks 🙏

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[@dr.jitendrajharbade5221]

Excellent lecture, thank you to all team members.....

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[@PharmaMedicinalChemistry2021]

Excellent & highly useful presentation on HPLC, Thank you mam.

[@bhargaviyatham6303]

Excellent very easy to understand and who prepared for newly in interview purpose will deffinately help ... Thank u for this vedio. .

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Thank u so much and ur explaining style is beautiful & easy to learn!

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[@SamarawuIbrahim]

You are doing great ❤

[@gaganrathore5455]

Thankyou ma'am, for HPLC method development explain in easy way 😊

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[@shribhagwan4539]

Thanks for sharing valuable information on different aspects of HPLC

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[@aniksinha3734]

Excellent presentation of different aspects of HPLC, Thank you mam.

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Thank you

[@BARATHKUMARK-cp7ll]

Thanks mam
You lecture was very useful for me

[@mydia125]

Thank mam for presenting such knowledge for chromatography..

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Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.

[@manukumarbhandoria2554]

Thanks a lot mam for the detailed presentation in such a lucid way.

[@samirkotkar1513]

Sanket want to talk same things

[@dr.nayanpanchani9015]

Very good explanation on method Development of HPLC

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[@ranjitpatil359]

Excellent💯💯💯

[@pharmasolutionpakistan7668]

Good job
i am a teacher as well as lab instrument trainer
i appreciate yu
your convying mthd z very good

[@saikumartallaparthi8090]

Tq lot of learning ponits for hplc
Ur video

[@manishbhoye4344]

Nice lecture on different aspects of HPLC method development.

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[@zahidakhan1304]

Very well explained...thank you ma'am 👍

[@indie1751979]

Very nice lecture on HPLC. Thank you.

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[@lawanyapandey1660]

Thanks mam
It was very fruitful for me

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[@satishkumarsingh3919]

Excellent lecture.

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[@janmahho1547]

Thank you very much for your efforts and do you have any information about validation HPLC?

[@06_soniyakundnani13]

Thanks for nice explanation mam its really helpful

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[@4fourty242]

Helpful information 👍🏻👍🏻👍🏻👍🏻

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Very nice knowledge full points👍

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Very good information and expline medam

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excellent lecturing madam

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Outstanding presentation 👏

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[@PharmaceuticalConcepts_Ojaswi]

Excellent madam.. Very nicely explained..

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Thanks a lot

[@bharatmahajan1753]

Good video for team thank you

[@KasulaGopal-k2r]

VERY INFORMATIVE

[@monashah4079]

Excellent explanation of HPLC

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Very thankful madam 💓💓💓💓

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Fantastic

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Great lecture.keep it up

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[@vinayakkumbhar725]

Nicely explained and very useful beginners

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Thank you. Share and subscribe. Also watch ruclips.net/video/7wAWve4Ekqk/видео.html

[@sonajigaikwad6863]

Very very excellent presentation thanks mam

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Nice video. Keep it up👍🏻

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Explanation is best.

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[@renganayakidharmarajan9257]

excellent presentation

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Many thanks

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Superb mam, 🙏

[@madhurahegde2603]

good information...

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Excellent lecture mam

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[@92dahman63]

very good post, i have a correction here, at 14:10 you said that you can separate RNA and DNA with size exclusion chromatography which is not true RNA and DNA can be separated by gel electrophoresis :)

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Also check article Rapid purification of RNAs using fast performance liquid chromatography (FPLC)
Insil Kim,1 Sean A. McKenna,1
2007

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Size Exclusion chromatography is also called as Gel Permeation Chromatography or Gel Filtration Chromatography based on Stationary phase.

[@RahulBangare]

Totally Good 😃😊

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Before commenting you should know what you are telling. By the way in GC, separation is not based on boiling point. It is based on affinity of samples towards stationary phase and mobile phase( career Gas)

[@subhajitsikdar7658]

Excellent lecture

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[@mangammollasrinivas6003]

Madam please du more videos regarding method development... What are the common problems we are facing while performing method development...

[@santidas1]

nice session on HPLC method development

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[@dorakondaanilkumar6044]

Thank you madam... good explanation...

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[@kirankaarumanchi]

Your explaining very nice madam

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[@ravirajlandage3580]

Excellent PPT and excellent explanation

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Very excellent

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Excellent lecture madam,,,, thanks🙏..........

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Excellent

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[@krish_krish354]

36 minutes chiral detector ion detector comes under which property detector bulk or solute

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Good lecture

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Nice video

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Interesting lecture

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Thank you

[@HariKrishna.vallepu]

Excellent mam.
Can you explain GC

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Salute to mam💐💐

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Very good

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Super explanation

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Thank you so much mam

[@krish_krish354]

What is the use of retention factor and gradient retention factor

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In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible due to the constant mobile phase composition used in isocratic methods.Gradient retention factor, k*, also known as average k, can be used to describe the chromatographic conditions. k* requires a range of 2 < k* < 10, similar to that of retention factor, k. If k* is below 2, there is potentially insufficient interaction between the analyte and the stationary phase, whilst above 10, the run times can be excessive, which can decrease laboratory productivity and increase solvent consumption

[@krish_krish354]

Column back pressure increases in short or long column17.48 min

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with longer column , column back pressure increases

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Very nice mam

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Thanks ma'am

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Most welcome 😊

[@krish_krish354]

How do we know that from structure our compound is ionizabale and non ionizabale

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Generally salts of acids and bases are ionizable in nature

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Can you explain what is column back pressure

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Column back pressure is back pressure due to Column. As mobile phase passes through Column containing stationary phase of fine particle (3 micron or 5 micron), the pressure is generated.

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Good

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Lee Sarah Anderson Jennifer Wilson Mark