The Agar Files - Inoculating Agar to Grain
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- Опубликовано: 15 окт 2024
- Today's class will go over your colonized agar dishes and putting them in grain jars in order to make grain spawn.
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Sensei! You’re the only channel on RUclips that actually gets me amped for a premier! Thanks for the quality content!
Same here
Yo so true
What do you do with the bird seed is it just raw from the bag or are they soaked or what ?
This is the most wholesome place on the internet right now, period. ✌🏻🍄 Thank you sensei!
Growing mushrooms is very satisfying. I'm about a month into my journey. It all started by being blessed with some fresh fungi n I made my first spore prints. Now I've just inoculated my first agar dishes! With a little more confidence I hope to be documenting and creating content to add to the interwebs, ty for the inspiration sensei!!
Sounds awesome, I'm curious how did you come across fresh mushrooms and do you know what species is it?
Gopher it!!!
Are you making content yet?
I was literally just on reddit where someone had an amazing flush using your methods, outstanding!
Methods he read on shroomery****
@@dethyl the real OG’s
@@dethyl whats shroomery?
@@michaelevans6216 its a fourum site for information pertaining to cultivation of mushrooms
I love it! I tell everyone you need to get into agar. Its crucial for mycology absolutely crucial
and it's a lot of fun too
For sure! I almost gave up after two failed attempts putting MSS straight to grain. So glad I gave agar a try. Will be using agar every time from now on.
Man, I love what you're doing with educating everyone and helping people find a new hobby! I'm glad I found your channel back when you only had 3 episodes out. Mush love homie 💙
Thanks for being an OG!
Yo i had no idea this channel is less than 6 months old......dude holy shit, i thought this was maybe a channel built up over 2 years maybe, by far the best mushroom channel on yt, ur amazing keep it up teach!
Thanks! I appreciate it. I put in a lot of effort on my videos 😃
@@PhillyGoldenTeacher yea we can tell bro. Thanks . Spread that knowledge!
Spawned my agar to grain 9 days ago and they're colonizing FAST! Was already about 25% at day 7 so I gave them a very good shake and now a couple days after the shake I have mycelium growing from all over the grains. I didn't even do any transfers, but I did have lots of rhyzomorphic growth straight from the start. Went from MSS to agar. And I had almost gave up after two failed attempts at putting MSS straight to grain. Agar is the way to go! I have more on the way and will be doing transfers next, can't wait. I'm hooked on this hobby now!! Thank you so much for your videos! If it wasn't for you, home mycology and willymyco I wouldn't have given this hobby a try! Yall three are my top three channels for mycology info!
So you unscrew the jars a tad & leave them; with temperature controlled space?
Thanks, this will be my first attempt and I’m stuck with Temperature issues.
Small space & on a budget 🙃….
Any possible ideas or suggestions for Temperature ?
A small space heater 🤔😅🍄🩷🙏🏻
@@remingtonfrog3170 I use modified jar lids with the little stick on filters. Or just drill the hole about the size of a pencil and use two layer of micropore tape. As far as temperature low to mid 70’s. I use a cabinet in my kitchen next to the refrigerator. The fridge puts off a little heat and the back of that cabinet is a wall and on the other side of the wall is my garage. So I get heat from there also. Usually right at 75F there.
Did you use a laminar flow hood when moving agar to grain?wondering if it’s necessary
@@Supremebeing47 Still air box.
The sound of you shaking the jar sped up, certainly got my cats attention, shes currently hunting the sound of your jar.
Keep up the Good work
Lol thats funny, my cat gets startled when I shake my jars.
Love waking up to find out that I missed class lulz love the series
“Yayyyy” haha always love the endings
Woohooo!! So thankful for you and everything you do for the community. Thank you PGT! 👽🙏
Even now that I've gotten this down , I still come back and enjoy this time and time again . Great content , thanks for doing what you do.
Very chill yet informative vid, thanks man 🍄
agar to grain was my fav method back in the day :) . But I had a good hermetically sealed glovebox.
I would drop one chunk per jar. I found that worked faster than spores anyway.
Your the reason my first grow is going so well. Thanks PGT, you’re necessary for the mycology community
Did this today for the first time. Pleurotus pulmonarius that I cloned to agar from a wild specimen I foraged, six perfectly clean cultures (even smelling wonderfully mushroomy) into three jars of popcorn. I'm so excited. Thank you for opening up this endeavor to me through your knowledge and efforts.
Your advice has helped me a lot, especially on fruiting conditions, got 60g dried out of a 1200 cc tub in 4 flushes. When I move to a new place ill start doing agar, thanks to your videos. You are a great teacher and I cannot wait for more videos!
Help! I keep getting wet contam, what was your process?
Quality content. Straight and to the point, no fluff. Keep it up chief. 👍
Lovin it PGT! Gonna get spores as well as clones going on some agar in the next week or two. Great lessons!
I just watched all your videos and they are awesome. Super informative and very soothing. Top notch! Really interested in learning about liquid cultures too so hopefully will see a video in the future from you!
Liquid culture is coming next month. I'm still testing out some recipes. Want the best for you guys.
PGT!!! Needed this vid for my first APE adventure I’m about to endeavor on. Perfect timing. Keep posting man your channel is the best out there and your knowledge is invaluable. Mush love!
*Zero thumbs down*
Me: Damn right
Thanks so much PGT, I found my love for mycology with you.
I just got my hands on a spore print and instantly found your channel. Have been passively looking for spores for a year or so, and now there's your video series. If this isn't cosmic alignment idk what is.
Thank you for your work!
A question: when working with prints, would simply using a burner to work underneath (instead of still air box) be sufficient? I fear it's only useful in Lab conditions, where inherent pollution is very low from the start, compared to the multiverse of things circulating around in my room.
Thanks again, and peace with you!
Still air box is best option unless you have a laminar flowhood.
Basic, simple and easy to follow content. Great Channel for this topic.
I've learned more from your videos than most all of the others combined. Love the way you explain things and make it super simple to follow!! Thank you for what you do, keep them coming.
Thanks a lot for your dedication, your passion and all your Mycology clases PGT ! I'm honored to learn all this knowledge from you and sponsored by amazing people! 🙏✨🍄
Best channel on getting started on Shrooms.They have changed my life and after a car wreck Severe Brain trauma My Life was a Mess.I wasn't into doing mushrooms but with all the nerve damage and Meds the docs had Me on was Crazy.Not helping at all. For 3 months now I fruited my shrooms and been micro dosing 4 on 3 off. Amazing what it has done. Thank You for saving my life.
Hey Mark where did you get your starter kit. been trying to find some but they're all in europe. any suggestions
@@samy92879 I don't understand the question Kit?I got everything to do it which is very expensive for real.I mean by the time you know it one has 1000 dollars into just basic stuff.Oder Spores syringe tubs Substrate torch scales knives vermiculite gypsum,coir, alcohol papertowels,I mean everything.making a still air box is 200 in itself jars grain lids.
@@samy92879 Try Canada Colorado,Oregon to name a few plaCES that have all one needs to get started.Shroom Supply,Quality Spores is a couple places I use.
@@samy92879 No kit order spores and start.
Hey there! I am new to this but utterly obsessed, you are such a wonderful source and your voice so soothing! Thank you 🥰🍄🙏
Everytime I watch one of your videos I always have something new to learn or take from it , and is ways explained in a manor I feel must can understand or learn from. Great video once again 👏
Thank you for the content! I have learned so much from your videos , and wish you ever expanding success!
Always pleased to get a notification about a new video of yours, you never disappoint 👍
I am new to learning how to grow Mushrooms. I have watched many videos on this on RUclips, all been amazing, until yours popped up. The way you explain everything in each of your videos, I find sO easy to understand. I have learnt so much in such a short time. Your videos are brilliant. Thank you for sharing your work and knowledge. P.S I have subscribed to your channel.
Thanks brother! It's working better than I ever expected! You've been a wealth of knowledge and you've helped me immensely.
I'm glad to hear of your success! Wish you the best on your journey! 🙏🍄
@harrisonvictor4916 it's laced with fentanyl
Sensei - you're the best! Thanks so much!!!!
Beautiful! So clear and easy to follow. Keep up the great work for all of us out there PGT!
YO PGT .... *please* do a SAB tek. do's and dont's etc. I'm getting way too much contams on agar. gotta be doing something wrong here. my SAB is quite small as well. very frustrating
I have 8 different mushroom varieties at different stages growing.
im up to 4 myself right now :)
Can you store agar w/ mycelium growth at room temperature for long periods? What is a good way to store it?
please keep making content T.T this is beautiful, thank you PGT!
Does loosening up the lid for gass exchange cause a risk for contaminatoin? Love your videos. Very to the point information
contam isn't good at turning corners
Dang....that Agar took off, especially compared to spore inoculation.
Thank you for all of the knowledge you share! 1st time I went with brf cakes and have been regretting it ever sense i saw your video on bods unmodified shoebox. I just knocked up 5 jars last night using broke boi tek. This process seems much better imo. Much love PGT! Canr wait for the next video!
Just about to try this for the first time, your excellent content is much appreciated!
Another great content creator in the community. Thanks for the great video and useful information!
"You can never be too careful, Sometimes" el PGT
Haha I caught that too :)
Hell yeah!
Love the content man, truly one of the best shroom RUclipsrs out there!🤙🏻
Keep up the great work
Thank you from morocco.god bless you
these videos are an excellent resource. much love!
Mush love 🍄
Hey sensei I love the videos. I was wondering if you would ever consider making videos on how to make and sterilize agar and how to use a teK WITH a pressure cooker. Keep it up brotha
This looks so much cleaner and easier than making syringe needles thanks b
Very good tutorials. Thanks.
What a qualitative content ! As always in PGTs class ! 🙏
thx for all your videos :) Whats the name of your funny little plastic AGAR Dishes or how are they called?
Thank you fro the hard work and dedication sensei
Hell yeah! Just gotta wait 11 hours, see y'all then
_3 months laiterr_ :o
looks clean, didn't realize it was this simple, I just need a instant pot
Haha my corn just finished in the pressure cooker (((: gonna let it sit overnight and innoc tomorrow
gday mate can u do a video with agar dishes on wheres the best place to sit em to speed up or generate best ideal spawn , cheers mate
Can you do a video on the bird seed jars?
Great content! Thanks PGT.
Very educational and well made video. You unscrew the lids of the containers to exchange air. As the containers are not in front of a flew hood, are you not afraid of contamination? This is only a storage for the containers in room temperature?
You are a blessing
thanks for caption because i'm brazilian is i know i'll learn a lot from you
Love the videos keep them coming your awesome!!! 2 questions how long can you store agar before adding a piece to clone also what bird seed did you use? Could I just use whole oats?
You can store them pretty long before using them. I've gone over several weeks with them sitting out empty before using them. And my wbs contains milo, millet, cracked corn and sunflower seeds. And you can use oats, they work just fine.
Thanks for the video! I always learn new things from you.
I read that it's possible to use dried mushrooms in ager and they will make mycelium, do you know if it'a true? and can you show us how?
Hey buddy i just grew my first golden teather using your awsome Skills they turned off very nice but i think that i have some potency problems my friend took 1.5gram and had a mild effect when i was younger 1.5gram of mexican blew my soks off what am i doing wrong is it the way i try them or just normal for this strain hope you can help me by the way love your Channel bedst regards Joe 🙏🙏🙏
Are you inoculating your agar wedges in front of a flow hood? I’m going to use a hepa filtered iphone work station for to minimize contamination. I’m growing Lions mane for personal use. It’s helped my cognition dramatically. It took a while to work but I’m eager to grow more. Thank you for your videos, teaching time and generosity.
Beautiful!!!
Thank you sensei 🙏
LOVE YOUR VIDS MAN
Great videos teach!
I'm surprised how dry those grains seem versus how well it works. Those jars sound like maracas!
Nice man! How and for how long can you store agar cups with mycelium? keep up the good work:)
Awesome channel! Thanks.
Absolutely awesome thank you
That fluffy snow mycelium is beautiful
Great video, thank you sir!
Can you take spoon of the “ready” jar and add to new jar to start up the process again? And how long is it good to store ? Thanks for your channel man great stuff
he has another video on this topic. Its called grain 2 grain transfer.
I'm days from taking the first leap and getting my feet wet. I have most of the supplies, trying to decide what grain to use...
I'm excited for you, I made that leap too now just getting ready to put my colonised jars to bulk sub, 😁
If you don't have a pressure cooker yet, or just want to get a fast start with pre sterilized grains. I used the 5 grain jars from midwestgrowkits and am having awesome results. Looks like a wild bird seed mix but doesn't have sunflower seeds or corn in it and it does have some rye grains in it.
I would imagine any nice whole grain that isn’t too small (like quinoa).The pre sterilized bags I got off of north spore just to get going for the first time was millet I believe and it’s been working good so far.
I'm trying different grains. I got corn, wheat, sorghum and millet. Apparently rye is the best but they didn't have that in the animal food store.
@@niklashultkrantz7766 Actually millet is best. Smaller, more inoculation points.
What's the next step after grains? I could not find a video in your profile.
Thanks for sharing 🙏🏽
can you put those new plastic lids in the pressure cooker?
At 1:40 when you said the amount you put will influence the amount of time it takes for the agar to be fully colonized. If I put more agar in the cup will I have a shorter or longer colonization time???
I live out near west chester great content man!
Love this guy
so my question for you is, can't you just do a spore syringe to one of your agar cups? that way instead of using a full cc of liquid in a jar you can just maybe put a few drops into an agar cup and use that to colonize a quart jar?
would one drop of liquid be enough to colonize a whole agar cup? or will i need to transfer to another agar dish to fully colonize. If one drop fully colonizes the top of the agar than id only need one drop of solution per quart jar. any info is great thanks.
Thanks you for this clear information. May I ask, your lids do not have any vents, is this not necessary, and do the lids need to be tight or loose please.
Im looking forward to your video about liquid ciltures
Coming next month, I'm still testing out out different recipes to see what works best.
I love your channel man:)
Very mindful... Very demure❤❤❤
namaskaram PGT-san, Awesome how-to vids!!! Q: is it possible to
create a liquid culture and go directly to bulk substrate, by-passing
spawning?
No you want grain spawn with your substrate, as it's the main nutrients for your mycelium. Substrate won't have enough.
Did you pressure cook the jars with those lids on them?
Ok im gonna ask a stupid question. After i put the agar in the grain jar does it need to be placed in a dark place or just regular light from a room or window ?
Just did my first philly special yesterday. Didn’t look like yours Sensei
I see you tighten the jar after allowing the gas release for a week or so first, Is it okay to keep the jar tightened after that for the remainder of the colonization?
First off, thank you for your great videos. I've watched quite a few mushroom growers on RUclips, and by far, yours are the best educational videos on the subject. Now my question: I am a first time grower. I have been trying to grow in a commercially purchased grow bag, but it's going very slow. I inoculated via spore syringe (3lb grow bag, 4cc spore water) , and after the first 2 weeks, I had nothing. At that point I said screw it, and inoculated with the rest of the syringe (an additional 10cc) into the same grow bag. I am now another 2 weeks later, and have a small mycelium patch growing (about the size of a quarter) on the bottom of the bag. I am keeping the temp around 70°f, and the bag in the dark. What am I doing wrong? I am trying to grow your namesake, if that matters.
Temps are fine, closer to 80f will get you faster colonization. But really it's all about patience.
@@PhillyGoldenTeacher Thanks for the reply. Being winter in the pacific northwest, low 70's is about all I can afford for heating. I may try putting it in a metal cabinet with a small space hater and see if that helps. What have you found works best...having them in the dark, or some indirect light? Thanks again for your great videos.
I have not been using enough agar. Thanks for the video
Thank you teacher I am in Canada, Montreal and I wonder where can I purchase lion’s mane spores to cultivate them at home
PGT, great video!
Curious - can those reusable mason jar lids be pressure cooked without damaging them?
Thanks!
Yes they can
@@PhillyGoldenTeacher Nice. Have you noticed any delay in mycelium growth using these lids say compared to using a lid with a filter patch on top?