Good HPLC column washing 🚿

[@lubuulwajames6938]

AMR kindly present a video on hplc Calib ration especially with regards to noise drift ,lampintensity test how it is performed using agilent chemstation on hplc

[@user-ew9bs5tt4b]

Thanks to your kind video:)
I think the time of every stage is determined by column’s size, is it right?
If it is right, how can I calculate the time?

[@karenperez5618]

Thank you , I have a question in my lab they put the column in methanol 50% for storage is that good practice?

[@worldofchromatography8151]

Yes,, but 100% Methanol is the best recommendation at the end of washing sequence, because presence of water for along time may decay silica

[@user-fs4lq5nd1w]

لو سمحت هل ده بيكون في نفس اتجاه ال in و out الطبيعي ولا بنعكس اتجاه ال column

[@worldofchromatography8151]

بيكون في إتجاه السهم

[@sohailasabryawad6619]

بس انا يادكتور جيت اعمل زي الخطوه الاخيره واعملwash ب methanol ١٠٠٪ لقيت ال colum ب leakage وطلع ان الcolum اتسد أو في bulk في نص الcolum هل ده من الmethanol 100% اللي عملته

[@worldofchromatography8151]

لو عملتي بنفس الترتيب اللي في الشرح فمستحيل يتسد بسبب ال Methanol،، الطريقة ده Recommended أصلا من مهندسين صيانة للأجهزة

[@charafeddine7095]

Why Meth and not ACN

[@worldofchromatography8151]

any organic