Fungi Friday #6 Testing the flow hood and calibrating the workspace
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- Опубликовано: 17 дек 2020
- In this video we test the new flow hood and show our technique for calibrating our workspace using petri dishes. We hope you enjoy our FungiFriday videos and we look forward to the next one! MUSHLOVE
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follow up reading here: ruclips.net/video/9gnU4wddDm8/видео.html
Calibration
Great video Gary. Planning to get a flow hood soon and will test as you have shown. Thanks
You're awesome Gary! Big thanks and much respect.
Hey Gary, I just wanted to say thank you for all that you provide to this community. Your videos are regularly referenced and recommended in different mushroom forums I am part of. Your expertise and willingness to share your knowledge is definitely not overlooked. I certainly appreciate you and I know that many others do as well. I hope to get a flow hood eventually, but for now I will master my craft in my SAB. Thank you and happy holidays to you and yours. 🍄❤️
what forums?
@@johnobrien7537 Philly Golden Teacher Discord, and I also heard it on the 90 Second Myc Discord before it was taken down. So I guess Discord “forums”. I never hear anyone speak anything negative about Gary. He is definitely well respected. There are other forums I can’t think of at the moment where I have seen him referenced in a positive manner.
Awesome video!
Thanks for all the wonderful content. I do have a question. Would it be better to not have the dead zone at all and have a shorter version of the hood? I work off of a tiny flow hood and definitely see the point in going larger as it's not as tall as a stack of strike dishes. What is the benefit on going with such a tall filter system?
Keep up the good work man!
Thanks MUSHLOVE
Such a wholesome guy
Hey mush love brother I appreciate your videos
thanks for watching and following along! MUSHLOVE
What are your thoughts on creating a plexiglass or similar roof and walls around the work area? Seems like it would improve your chances of sterility?
Thank you sir
Which is the composition of the medis from Petri dishes , to test the sterility of cabinet flow and your installarion ?
so it’s definitely bad if the flame goes out during the lighter test?
That flow hood makes my mushroom grow.
What does DRBC plates mean or stand for?
Hi Gary, I’m a myconewbie and am working (playing) to educate myself. I’ve enjoyed watching your videos and have just subscribed. Thank you for your instruction. I’ve looked for the next video to see the results of this test and can’t find it. Can you direct me? Thank again!
Hey thanks for watching here are the results ruclips.net/video/9gnU4wddDm8/видео.html we have tested regularly and the only issue seems to be the pre-filter getting gummed up but we clean ours under soap and water and let dry every 6 months and it has been working great with this regiment
You are a smart man. Did you take mycology courses or any science post secondary?
I appreciate the kind words, although I am merely a student to the mushrooms : ) My background is in Medical Laboratory Science (Clinical Mycology/Microbiology) and I have been learning on my own since I graduated from UB in 2013 with my undergraduate degree.
@@FreshfromtheFarmFungi Haha that’s awesome. I watch your videos and you use words outside of the regular “mycology” vocabulary. Great job with your videos and explanation all around. Really digging them! I appreciate your videos very much
How did you cancel the noise from the fan in this video? That's amazing.
I tightened up some rivets and put a piece of foam against the pre filter so it doesn’t rattle - this is one of the quietest flow hoods Ive used so far! Very pleased 😀
how you compare your new flow hood with your old one in terms of efficiency ? Thanks
I like it for bulk inoculations (the new hood) but still do my culture work in the vertical flow hood it seems to have better results but it might be my bias from using it for so long
You dont like the top flow anymore ?
I was under the impression the Laminar flow coming out, was supposed to have quite a push, even enough to blow out a flame. Am mistaken ??
this works perfectly fine - anything with more force is overkill in my opinion. It may work but is not necessary
@@FreshfromtheFarmFungi
Ok thanks that clears things up for me.
It's very quiet as well!
I'm confused. Why can't I used my mea agar plates? Do you mean the time being a factor because what's the point of using a flow hood if my mea plates will catch contamination?
you can use MEA but the DRBC are specific for yeasts and molds - MEA are more sensitive
Same question. What's the point of s dope ass hepa hood if you can't work woth MEA...?
Hi there!! One question... what’s the cfm rate of the fan back there? Do you you think that this set up would be good for tissue culture? 🌱Thanks👍
I'm not sure on the specs but from my experience so far (poured over 300 plates without any contams and did over 25 transfers in the first 2 weeks with no contams) it seems to be sufficient for mycology, which in my opinion is more liable of a process because most plant tc media contains less nutrients and more inhibitors. I would use test tubes to be safe but I believe it's a great setup - only time will tell for longevity, but the pre filter is still clean after 3 weeks now and I usually swap them every 6 months. If you are willing to spend the money, the air science flow hood has been going strong for over 3 years now and that is what I used regularly for plant tissue culture in the past. It's about 3x the cost for the same size (4' hood)
@@FreshfromtheFarmFungi im thinking about investing in the 72" ULPA hood.. thoughts ?
What is the primary difference between 2 HEPA filters with a similar rating (99.99% 0.3micron) of a different thickness? You said that the filter element on this one from Myers is 2.5" thick. How does that compare to filters that are much thicker like this 11.5" thick filter? www.filtersfast.com/p-HEPA-Filter-Replacement-Camfil-12XH24Z24Z121D3CA.asp Less resistance & more air flow with a similarly rated blower? Longer life expectancy? Cheers!
The thickness affects the surface area and that affects the CFM rating for the fan and the air volume pushed out from the filter
The thicker the hepa filter, ( more surface area) will last longer than a thinner hepa. A friend of mine owns a filtration company and taught me this. I have also verified this in the field as a service tech.
@@brucekidd6133 That's what I figured, thanks! :)
was there a follow up on this?
it was clean ruclips.net/video/9gnU4wddDm8/видео.html
I bought one of these form a dude in oregon, 12 hour round trip, $850, 2x4. Experiencing contamination. bought an anenometer for 15 bucks, 70% of the filter is dead zone, the other 30% is 180-240fpm. Contacted him, he said to seal with silicon. Just did that, fingers crossed it helps but my faith level is low. Expensive mistake if so, may have to build a true laminar flow hood from scratch.
look up the mac10’s by humble fungus -another good alterna
Did the silicone sealing help?
@@naturallydope247 nope, still turbulent flow. Makes me kinda sad, the person is ignoring my latest attempt to reach out and doesn't seem interested in remedying the situation. Funny that you should comment now, I am currently as we speak working on building my own 2x4 laminar flow cabinet using FreshCap's guide. Almost done :)
@@samuelmonteon1430 I'm sorry you had to experience that. That's rough. When I priced out the cost of buying one premade vs just making my own it was just more economical to make my own. I'm also almost finished mine, but it's smaller for my workspace. I started making the list for my next one though-a larger 24x18 size. Best wishes with it all!
@@naturallydope247 It's ok, I've moved past it mostly lol. Well it's not the size that counts, it's how you use it XD If I was building from all brand new pieces, I would have probably went with a smaller like 1x2. However, I just so happened upon someone who had a 2x4 HEPA and the fan motor for it that was willing to give me a decent deal as they were moving out of state, so it was just my luck! It was funny, found them through an ad for metal racks for our microgreens, they ended up having microgreen supplies to sell us too! Thank you, Good luck on your build, hope it comes out great!