Gram Staining Procedure Animation Microbiology - Principle, Procedure, Interpretation
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- Опубликовано: 24 сен 2019
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Gram staining procedure animation - Principle, Procedure, Interpretation
Principle:
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Staining is an auxiliary technique used in microscopic techniques used to enhance the clarity of the microscopic image. Stains and dyes are widely used in the scientific field to highlight the structure of biological specimens, cells, tissues etc.
The most widely used staining procedure in microbiology is the Gram stain, discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction. Gram-negative bacteria are decolorized by the alcohol, losing the color of the primary stain, purple. Gram-positive bacteria are not decolorized by alcohol and will remain as purple. After decolorization step, a counterstain is used to impart a pink color to the decolorized gram-negative organisms.
Importance of a Gram Stain:
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The Gram stain is a very important preliminary step in the initial characterization and classification of bacteria. It is also a key procedure in the identification of bacteria based on staining characteristics, enabling the bacteria to be examined using a light microscope. The bacteria present in an unstained smear are invisible when viewed using a light microscope. Once stained, the morphology and arrangement of the bacteria may be observed as well. Furthermore, it is also an important step in the screening of infectious agents in clinical specimens such as direct smears from a patient.
The Gram stain procedure enables bacteria to retain color of the stains, based on the differences in the chemical and physical properties of the cell wall.
1. Gram positive bacteria: Stain dark purple due to retaining the primary dye called Crystal Violet in the cell wall. Example: Staphylococcus aureus
2. Gram negative bacteria: Stain red or pink due to retaining the counter staining dye called Safranin.
Example: Escherichia coli
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📌𝗦𝘂𝗯𝘀𝗰𝗿𝗶𝗯𝗲 𝗧𝗼 𝗠𝘆 𝗠𝗮𝗶𝗹𝗶𝗻𝗴 𝗟𝗶𝘀𝘁:- linktr.ee/DrGBhanuprakash
This just explained it to me in 4 mins what my teacher couldn’t do in an hour! Thanks a lot 😇
You're very welcome!
😂😂😂😂😂
True'😶😂
Mine teachers couldn't understand it in a year 😂
True 🤣
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I have seen many videos but this one is the best
thank you
Legend watching in 2024😂😂😂😂😂
Sir Your explaining topic vry clearly & interesting way through animation, I'm B.pharma graduate, still i have doubt about staining, i understand in 4minutes. Thank u Dr. Bhanu prakash sir.
Excellent presentation, this will allow my students a clear understanding of how the mordant works in Gram staining. Thank you.
thank you sir for this video I understand this concept in just 4-5 minutes that i am trying to understand from half an hour.
Great video with great detail. Thank you.
The animation is great, thanks for sharing it, it will help a lot to the courses and classes that I teach at the university (UCV). I will always take it into account and generate the reference quotes for your recognition.
really thank you for such valuable information.
Glad it was helpful!
This explanation was just amazing. Thank you for the video
Thank you. It’s really helpful
So much faster than reading my Micro textbook... thanks a LOT, Sir 😁👍
Glad to help!
Very precise and understandable. Thanks
Bundle of thanx for excellent presentation
Thank u for this video....👍🔥
it helped a lot. thank you.
On point. Asante
Very use full
Thank you for the presentation, it will be very heplful to my students ( with reference quote for you)
Ur most welcome
Thank you so much ❤️❤️
Thanks a lot. This has made made understand it very well
It is so helpful!
Amazing
Best video ever
Thank You Mam
Very fruitful❤
Ty sir
Like your way of explanation...and voice
Thanku sir ❤️
Thank you it is help to us
Teşekkürler hanımefendi çok iyi anlatmışsınız
Thanks❤
Thank you for your presentation it is very helpful for my students to understand gram stain
It's my pleasure
Thanks
Thank you 👍
Day before exam❤
About to start bsc microbiology. How was the exams?
Thanks 👍
I understood thank you
Thank you
Excellent explanation
Thank you so much!!
Ur most welcome
Superb
Clearly understood thank you
Same 2 u maryam, do you read in 11th class?
Thankyou so much for this video! Now I feel ready to score full in my Biotechnology Practical tomorrow! The greatest thing is not just the animation but also those little concepts in every step ! Thankyou so much once again!!
Glad it was helpful!
It indeed was !
@@doctorbhanuprakashit was really helpful thanks a lot
Very helpful
Nice vedio
Everything is clear
Thank you so much
Most welcome 😊
A short video and useful to be learned on it
Good job 👍
Glad to hear that
best
Thanks it helped me
Good luck on my practical exam
Am happy to see this because am a microbiology student passed out student
دكتوره خديجه رفعت مشاهداتكم
This is super helpful thank you very much it really helped
Glad to hear it!
Good discusing
Thanks 🙏 that helps a lot.
🤝🤝🤝
Animation are to good, Thank you👍
Glad you like them!
thanks for your short and précised procedure
You are welcome!
Amaaaaaaaaazing ❤❤❤❤❤❤❤Thank you so much for this helpful video ❤
Glad it was helpful!
Woow👍👍
Great video👏👏,I'm sending this to my students as they had doubts on this topic
Glad it was helpful!
nice
Great video! Thank you
Most welcome
very nice slide
thanks
Amazing video. Thank you
Ur welcome
Thanks this video help us a lot to understand 🤩
Glad it was helpful!
Super lecture..tq so much
Pls do upload all bacteria
Suree
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Some video not show why
Please, could you suggest me the software you are using to creat such animation videos.
.
Very precise video❤
Glad you liked it
Thank you so much for this video
Most welcome 😊
Crystal clear
Thank u
Very nice 👌😊👌😊👌😊👍
Thank you❤️👍
You’re welcome 😊
Nice
thank u so much..
Welcome 😊
Great Explanation. Thanks...
Ur most welcome
It's really very helpful
Tysm
Any video abt acid fast staining
What happens if you use acid alcohol as the decolorizing agent in spore staining?
I am Bangladeshi...I am a student of microbilogy..This is very helpful video for me
Thanks.👍 from sri lanka
thank you this is very good video
Glad you liked it!
Nice Video Explaned in Easy way Thank You
You are most welcome
Thanks 😊
Welcome 😊
Best animations
Tqu
Having lab test today thank you ,stressed out right now
You got this!
thats fascinating
Tysm
Thank you sir
Welcome
Nicely presented,, thanks for sharing this video😊
My pleasure 😊
Microbiologist👍
Thank you 😊
You're welcome 😊
Excellent video
Tysm
Thanks you mam
Most welcome 😊
Very well Explanation ❤
Glad you liked it
Why must we dilute the samples from the agar plate?
Great video 💞💞 can you inform me the software used
Sir i have herbal extract liquid which having bottom ppt and i want to check this ppt is by fermentattion or not so which testing is best in lab for detection can you tell me bcz i send this sample for testing
Please tell me to prepare smear and microscopic examination of staphylococcus lactobacillus and e coli and gram staining teqniq both are same please tell me tomorrow is my practical 😭😭
wow
Good explanation 😊❤
Thank you 🙂
@@doctorbhanuprakash u deserve this explanation is just amazing
En mi experiencia realmente no es necesario cronometrar exactamente el tiempo con cristal violeta, iodo lugol y safranina debe de dejarse mínimo un minuto, pero realmente máximo tiempo no hay, y la decoloración no debe de ser hasta que deje de escurrir cristal violeta porque muchas veces y aunque se usen cepas jóvenes, se corre el riesgo de que se decolore a la fuerza, lo mejor es dejar derramar el chorrito de decolorante desde el inicio de la laminilla y permitir que llegue hasta el final de la misma, pasando por eso frotis ( que debe ser de buen tamaño y grosor) y de inmediato enjuagar. Crees ese es el "secreto" para una buena tinción.
People watching in 2024 ? For Neet ?
For biotechnology
For lab😅