How to do single-cell RNA velocity analysis | Complete introduction

Complete introduction to single-cell RNA velocity using scVelo and velocyto. I explain what RNA velocity is and show you how to do single-cell RNA velocity analysis on your own data. I use velocyto and scVelo to calculate RNA velocity and project the velocity onto UMAPs. I also show how to find the gene velocities of given clusters or individual genes.
References:
velocyto.org/
scvelo.readthedocs.io/
Notebook:
github.com/mousepixels/sanbom...
0:00 intro
0:20 What is RNA velocity
3:01 Installing velocyto
5:10 Counting spliced and unspliced RNA with Velocyto
9:00 Installing scVelo
10:00 Basic scVelo usage
21:13 Ideal RNA velocity example