GraphPad isn't letting me chose Tukey's method. It only lists Dunnett's T3 test and some others that are not Tukey's method. Is there a way to force the software to let you chose Tukey when it doesn't list it as a recommended test in the options tab?
You first have to plot a standard curve, that is measure the absorbance of standard of known concentration at different dilution levels. Make sure your data is all baseline corrected (subtract a blank). Then plot a linear regression of absorbance as a function of concentration. Once you have the equation for the linear regression, you can plug in the absorbance of your unknown and use algebra to solve for the unknown concertation. Hope this explanation helps you @drmohamedsameh6292
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Why is the “SE of mean diff” the same for all the groups? Should we report that?
GraphPad isn't letting me chose Tukey's method. It only lists Dunnett's T3 test and some others that are not Tukey's method. Is there a way to force the software to let you chose Tukey when it doesn't list it as a recommended test in the options tab?
you got it?
How to extrapolate the concentration values from absorbance values
You first have to plot a standard curve, that is measure the absorbance of standard of known concentration at different dilution levels. Make sure your data is all baseline corrected (subtract a blank). Then plot a linear regression of absorbance as a function of concentration. Once you have the equation for the linear regression, you can plug in the absorbance of your unknown and use algebra to solve for the unknown concertation. Hope this explanation helps you @drmohamedsameh6292