Acid-Fast Staining of Sputum Sample

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  • Опубликовано: 7 сен 2024
  • Kinyoun stain to visualize Mycobacteria. It is similar to the Ziehl-Neelsen Stain, but it doesn't require heating during the Carbolfuchsin step.

Комментарии • 29

  • @marcingogowski6077
    @marcingogowski6077 2 года назад

    thank you - that's what I needed

  • @Apwbdtosct
    @Apwbdtosct 9 лет назад +2

    Thank you ! you just helped me score an ace on my internal assessment practicals :D

    • @bruxamistica5580
      @bruxamistica5580 3 года назад

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    • @bruxamistica5580
      @bruxamistica5580 3 года назад

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  • @asmita7738
    @asmita7738 2 года назад

    Thankuh for better understanding! 😇but plz its a humble request to turn Off the Tap in next time, plz save water! 🙂I really feel uncomfertable from that! 👍🏻

  • @nandhininandhu2699
    @nandhininandhu2699 3 года назад +1

    Sir pls close the tap while ur not using water. Otherwise ur explanation and demonstration is awesome. Thank you for this video sir🙏🙏

  • @JacksonReynolds
    @JacksonReynolds 9 лет назад +2

    You can alternatively use brilliant green as a counterstain if methylene blue is unavailable.

  • @medico958
    @medico958 2 года назад +2

    Every things fine but that running tap is giving me anxiety would've not let it run when not in use

  • @satriahadjobaru2718
    @satriahadjobaru2718 11 лет назад +2

    yes that too,and after Carbolfuchsin
    but doesn't it need to heat after Carbolfuchsin and methylen blue?
    what is the different between using kinyoun?

  • @markmicrobiology
    @markmicrobiology  11 лет назад +2

    It does sound like chemwipe, but Kimwipes™ are made by Kimberly-Clarke®

  • @munnukumar6748
    @munnukumar6748 3 года назад +1

    Nice 👍🏻

  • @deengee_
    @deengee_ 6 лет назад +1

    can I use malachite green instead of methylene blue?

  • @satriahadjobaru2718
    @satriahadjobaru2718 11 лет назад

    you didn't heat the excess water instead of shake the glass?

  • @siddharthannandhakumar6187
    @siddharthannandhakumar6187 6 лет назад

    How is the Carbol Fuschin taken up by the acid fast bacilli without heating ?Will it penetrate the Mycolic acid as such?

    • @markmicrobiology
      @markmicrobiology  6 лет назад +5

      Kinyoun carbol-fuschsin penetrates the mycolic acid because it has a greater concentration of phenol and basic fuchsin and therefore does not require heating.

    • @siddharthannandhakumar6187
      @siddharthannandhakumar6187 6 лет назад

      markmicrobiology Thank u :)

  • @Khaledf
    @Khaledf 6 месяцев назад

    Falmming ?

  • @markmicrobiology
    @markmicrobiology  11 лет назад

    Hi Satria. To which step are you referring? It should be dried before the final before viewing it under the microscope. Are you referring to that step?

  • @mohamddhiidhaco5022
    @mohamddhiidhaco5022 7 лет назад

    These steps are not standard method

  • @Raneyrogers
    @Raneyrogers 11 лет назад

    Isn't it spelled "Chemwipe"? It's all good though. haha
    Good video

  • @mosesrongmei650
    @mosesrongmei650 6 лет назад

    U forget the heating process

    • @markmicrobiology
      @markmicrobiology  6 лет назад +2

      Hi Moses, you would be correct if this were the Ziehl-Neelsen Stain, but it is the Kinyoun stain, which doesn't require heating. Sorry for the confusion.

    • @mosesrongmei650
      @mosesrongmei650 6 лет назад

      @@markmicrobiology Thanks

  • @itskcibaby
    @itskcibaby 7 лет назад

    you aren't supposed to do it over a sink