Thanks for the detailed instruction. It is very helpful. I encountered a problem when I was building a frozen tissue microarray by using the kit. I used the mold to build a base with 2mm holes and punched 2mm frozen tissue. But somehow the frozen tissue is larger than the hole and can't be pushed into the hole. Is here any solution for this? It looks from you video that the liver tissue is soft, is that why it is easy to be pushed into the hole? Thank you.
The tissue is frozen. But I believe my needle from being in and out of the cryostat warmed up so the tissue punch core inside in the needle thawed slightly. If you use needles other than the ones we recommend you can have smaller or larger core sizes. If you do have questions please email Arraymold. We try and answer all questions regarding tissue microarray construction.
Thanks for the detailed instruction. It is very helpful. I encountered a problem when I was building a frozen tissue microarray by using the kit. I used the mold to build a base with 2mm holes and punched 2mm frozen tissue. But somehow the frozen tissue is larger than the hole and can't be pushed into the hole. Is here any solution for this? It looks from you video that the liver tissue is soft, is that why it is easy to be pushed into the hole? Thank you.
The tissue is frozen. But I believe my needle from being in and out of the cryostat warmed up so the tissue punch core inside in the needle thawed slightly. If you use needles other than the ones we recommend you can have smaller or larger core sizes. If you do have questions please email Arraymold. We try and answer all questions regarding tissue microarray construction.