Tips for Bradford assays (or other microplate assays) without bubbles

Bubbly Bradfords? (Or other microplate assays?)
Those bubbles can mess up the plate readings, so the best thing is to avoid forming them in the first place by:
* Making sure your pipet doesn’t come out of the liquid when you’re mixing to avoid introducing air
* Setting the pipet to a lower volume to mix and/or only sucking up partway when mixing
* Pipetting slow
But, if they do form, all hope’s not lost! Try…
* Giving it a min or so to pop on their own
* Tapping the plate down gently on the benchtop a few times (gently, avoiding getting drops on the lid or spillover)
* Agitating it with a plate shaker (gently, avoiding getting drops on the lid or spillover)
* Giving it a quick spin in a centrifuge
* Popping them with a needle (carefully, avoiding stabbing anyone/thing)
More on Bradford assays: bit.ly/proteinmeasuring
More lab tricks & tips: bit.ly/lab_tricks_page & • Practical lab tips & t...
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbiochemist.com

[@darkconspiracy559]

Such a pain in the A** for mixing in pcr tubes specially !

[@alamie]

When using the same syringe between different wells how can we avoid contamination? If it’s just used to pop the bubbles is that okay? Does contamination even matter for the Bradford assay?
Apologies for so many questions, great video once again! 💖

[@thebumblingbiochemist]

Thanks! I don't worry about it for this. Any contamination would me miniscule.

[@alamie]

@@thebumblingbiochemist Thank you!