Coronaviruses 101: Focus on Molecular Virology
HTML-код
- Опубликовано: 6 июл 2024
- In this video, UC Berkeley professor and IGI Investigator Britt Glaunsinger, PhD, explains the evolution, genetics, and virulence of coronaviruses.
The rapid spread of SARS-CoV-2 and the resulting COVID-19 pandemic have highlighted the importance of studying the genetic landscape and molecular structures of coronaviruses. Hear Dr. Glaunsinger break down the latest scientific understanding of these viruses and what questions remain unanswered.
This video is intended for a scientific audience with an understanding of genetics and molecular biology. Dr. Glaunsinger gave a more lay-friendly talk on viruses in general for iBiology, available here: • Britt Glaunsinger (UCB... 
Наука
INTRO
0:19 - Human CoVs; CoV spillover to humans
5:13 - SARS vs. COVID19
8:45 - Talk overview
1. ENTRY
10:10 - CoV structure; spike conservation
14:41 - Spike and ACE2 interaction; protease cleavage
18:30 - CoV-2 spike residues
2. REPLICATION & GENE EXPRESSION
20:08 - 2019-nCOV annotation; 14 ORFs and 27 proteins
24:05 - Ribosomal frameshifting
26:38 - Sub-genomic mRNAs with shared 5' and 3'
29:06 - Shared transcription regulatory sequences
32:13 - CoV replicase; polymerase, capping and proofreading
34:08 - Exonuclease; viruses with >20kb genome
36:14 - Loss of Exonuclease; lethal mutagenesis
38:58 - Exonuclease and methyltransferase
3. Replication-transcription complexes (RTCs)
40:54 - Interconnected double membrane vesicles
43:32 - Vesicle formation
44:32 - Proximity labelling; Characterizing RTC proximal proteome
50:20 - Accessory genes
52:01 - Assembly of nucleocapsids into virions
4. Immune interactions
53:31 - Little or no interferon in cells; delayed interferon singling
57:39 - Short lived antibody and memory B cell response
OUTRO
59:38 - Key open basic science questions
thanks for the timecodes, much appreciated!
Its great to have time codes for a lecture.
Q: Is sunlight able to kill viruses and bacteria? Does humidity affect the transmission?
I've seen published medical documents that dispute what msm is issuing by script.
Thank you
Virusses do not exist, they are exosomes. Dr Stefan Lanka, Dr Andrew Kaufman, Dr Thomas Cowan. Many many others
I took a virology course in graduate school almost a decade ago, what an incredible distance we have come. As a microbiologist myself I really appreciate putting your lecture up.
Sir.i dont know about virology.But i hav a doubt can the caspid remove from RNA of virus without any damage?
Sir, i dont know about this subject.but a doubt can capsid remove seprately from RNA without any damage in capsid?
@@nbox9570 I think this capsid literally assembles on the RNA itself, so I would think it unlikely.
How do we know the genome of the virus?As someone on the west isolated or finally done the 4 gold standard tests? We were just told the Chinese mixed RNA from the liquid in 7 of the lungs of 200 infected with whatever. But had a test before they had anything tangible. Can't believe anything they say. So who an when was wuhan a factor. There's possible cases from September! ? Só why bat nonsense is being talked about its not real science face it.
No pangolins? What was the intermediary? Not straight from bats no matter how good the novel.
Despite having no background in medicine or biology etc, I found this lecture really informative and easily understandable
The manner in which material is presented is exceptional. To pack such amount of information in just one lecture is evidence of years of teaching the best students.This is an excellent example of excellent teaching for all teachers in academia
Thank you for this. I am a Candian biochemist stuck in Brazil. I decided to help the local authorities out. This brings me up to speed. I shared it among the English-speaking doctors as well.
Prof. Glaunsinger is a brilliant teacher. This presentation was extremely coherent and well put together. Thank you.
@Fat Albert How many Chinese students would be lost at Berkley if she did?
@Fat Albert Perhaps because circumstantial evidence is for conspiracy theorists, not scientists.
@@TopGunMan "Circumstantial evidence is used in civil courts to establish or refute liability. It is usually the most common form of evidence, for example in product liability cases and road traffic accidents. Forensic analysis of skid marks can frequently allow a reconstruction of the accident. "
Guess investigators and lawyers are just a bunch of 'conspiracy theorists' too.
Virusses do not exist, they are exosomes. Dr Stefan Lanka, Dr Andrew Kaufman, Dr Thomas Cowan. Many many others
@@Stefan-gh7xr all quacks
This is one of the most amazing talk i could ever come across about the structural details of Corona virus in context of mutational potential and probable targets for drug development. I might borrow some knowledge for my own understanding and spreading of awareness in here countries bereft of educational and research facilities and governmental intent for it. Thank you for this Leviathan of a task.
What a beautiful, clear minded, articulate, logical, level, educated, balanced, reasoned and highly intelligent voice. Very many thanks!! A perfect lecture.
Amen!
Even not being among the intended audience, i´ve been able to follow most of the lecture and have learnt quite a lot from it, and it´s really interesting, many thanks
should be on primetime tv
@ferzy09 if anything, this proves you didnt´t understand it
@ferzy09 actually not
A deeper look into any form of nature is fascinating, and we all would benefit from a better understanding in many ways. Better appreciation for our place in the biosphere, more insights into maintaining health, and maybe, just maybe, some insights into the human brain which could lead to improved social behavior.
@ferzy09 Nothing is going to prove intelligent design. The only thing that might cast a measure of doubt on evolution by natural selection is a bio-mathematical approach which shows that 3 billion years just isn't enough time to generate the observed (and growing) level of complexity in organisms. Unquestionably, evolution is a real and powerful mechanism. There may, however, be some surprises that lie ahead. (There always are!). All theories (including Darwin's) have a range of convenience.
Thank you, Britt, for producing this outstanding review. I'm sending the link out to our colleagues. (Dave Sulzer, Columbia Medical School)
Thank you, Dr. Glaunsinger, for that excellent introduction.
I was looking for something like this from some time ... Thank you for providing the authentic scientific information amid this info-demic where research data is hard to find on these media platforms 👏👏👏
As an undergrad biology major, I really enjoyed this in depth and straight forward overview. Thank you for making and uploading this and allowing me to apply and enhance my knowledge!
Studying viral biology at UG level. This is a really straight and detailed presentation. Thank you.
Thank you very much for this awesome lecture!
Thought I know a thing or two about gene expression, but I stand corrected. This stuff is just mind blowing.
Trust me. This whole hour of the video is worth watching. The professor details every key note about coronavirus. (I am not a virologist).
Great lecture. Very informative. I would love for Dr. Glaunsinger to give a follow-up lecture. Now that it has been almost two months with 10x more cases and more information I would love to hear her take on what's happening now.
This explanation was amazing! I'm an undergraduate student, only in my 2nd semester of biochem and was able to keep with a lot of it because it was very well explained. I loved it, thank you, and also thank you to all the scientists working tirelessly to fight this.
Isn't this the deepest RUclips video ever? Britt is the Goddess of viruses
Thank you for posting this Dr. Glaunsinger. It was just what I was looking for to understand the mechanisms of the virus, and how Remdesivir might work.
Many thanks for this. As a clinician, this sort of exposition is incredibly helpful in gaining a better understanding of what we are trying to manage (treat?). The link between basic science and clinical medicine is always a challenge and this sort of background knowledge is invaluable when thinking about what we are seeing in patients.
This is an incredibly fascinating presentation. I am so grateful for your commitment to the in depth understanding & logical explaination of this data to both the scientific & lay community. Phenomenal work!
Well done. Refreshing to see some virology 101 long-form presentations to counter a lot of the misinformation out there.
amazing explanations ! thank you
It took me complete 4 hours 45 min to understand this 1 hour lecture
Likewise!!
LOL don't feel bad it takes me about 5 hours to understand a one-hour lecture also LMAO
Really? Took me an hour mostly because I'm a freak for zoonotic viruses! 😂
Thank you very much Dr. Glaunsinger for sharing your outstanding presentation! It is very detailed and understandable!
This is fantastic, thank you so much for sharing.
Thanks for this lecture. Very thorough! Wondering if the more conserved fusion domain of the S-gp, or the polybasic linker would make a good target for mAb therapeutics?
Great discussion, I am an Immunologist. I learned a lot from listening. Thank you.
Thanks for doing this. Watched full video.
Absolutely superb lecture by an expert with encyclopedic knowledge of molecular virology. A very interesting and informative video.
Thank you . Was looking for this information for all long time.
Thank you, Dr. Glaunsinger, for this lecture
Wonderful lecture, many many thanks for your efforts.
this helped so much with my virology case study! thank you so much
@51:25 Please elaborate more on the association of INF and role of IFN inhibitors on viral accessory proteins.
Very interesting and thorough lecture. I learned a lot even without taking cell molec!
Thank you so much for this presentation. Im very far away from being the intended audience but it has been very informative in a time where we hardly get detailed information on what we are facing
You are so well read. Thank you for sharing.
fantastic video. great visual aids. also link to more lay info. superb
Awesome lecture! Could you please upload more about Coronavirus? I would love to learn more about it in a molecular level. Thank you so much!
Absolutely fabulous you presented the science extremely well, until we understand fully the biochemistry we cannot create a cure. As a former biochemist and now a doctor. I applaud you on a fabulous presentation.
Dear Britt, what is exact role of Furin in the process of Covid19's lifecycle/entry into human cells? Please shed some light on this aspect.
Thanks for this video. I sent it to many of my colleagues.
Thank you so much! This is very informative yet easy to understand! Nice presentation!
I wish this could qualify for CE credits
Outstanding lecture, Dr. Glaunsinger!
Thank you very much Dr. Glaunsinger for such a great lecture
An Outstanding Lecturer. Thank You Professor Britt Glaunsinger.
Can anyone share the time stamp for when she addresses how a respiratory virus penetrates the hosts brain cells causing them to run out and buy all the toilet paper? Or is that a different lecture?
Lmao
It infects spinal cells and moves to the brain through them.
the mechanisms by which virus genomes rely on their host and how the transcription machinery is modified is really fascinating.. especially the discontinuous transcription and the Exon protein... . I enjoyed this very much, thank you.
lecture seems very well put together, but it's really over my head without some background information, but I can see the work involved with putting together such an informative lecture, which is appreciated. :)
Cool.. Free Lecture. High Quality. God Bless You
Excellent...quick updates. thank you
Great explanation....stay safe everybody 💫🙂
very informative and sufficient in depht current knowledge, helped me a lot. Thank you so much Britt :)
Excellent presentation, thanks for posting.
Thank you for the amazing lecture. It was easy enough to follow despite my limited comprehension when it comes to sciency stuff.
pure gold, thank you!
very nice, thanks for the explanation. Can't we get the PPT ?
this is amazing work! Thank you !
Very informative lecture! Thank you, Dr. Glaunsinger
This was a really informative and well-presented lecture, thanks for sharing! All of our accumulated knowledge on coronavirus biology really stresses the importance of funding basic research on infectious diseases even when they're not yet an immediate threat.
Thank you so much for that lecture Dr Glausinger and IGI team. I was wondering if we are going to be able to figure out with certitud about the intermidiary species that hosted CoV-2 before it jumped into the human species? Is it a matter of more data/research needed or is it like a piece of the puzzle we will never know for sure?
What a lecture??
Not only in context of Covid19 but also in understanding to pathophysiology of virus infections challenge in the future ahead after current drastic challenge.
I recommend for incorporation of it in the pathology Curriculum word wide.
NEW Normal scenario paradigm 🤗
Very Brilliant of your Discussion Professor. I would be interested to know, what are the underlying aspects of such tremendous diversity in viral strains.? can we attribute the phenomenons of Epitope transformation and genetic variability due to absence of proof reading mechanism.? or some species mediated interactions in response to which viral pathogenesis has become stronger.? and do we have some counter act approaches to fight these virruses.? I would like to have your expertise in this regard. Thank you.
Great lecture, thank you!
Awesome lecture, thanks a lot!
really excellent ... easy to digest and clear ... big thanks!
This wonderful lecture is well above my level of comprehension. It would be great if Dr. Glaunsinger could present a youtube lecture addressed to the lay person showing how SARS CoV-2 could be defeated.
This is an amazing lecture!!!!!
Thank you! Dr. Glaunsinger, this has been an incredible lecture! Easy to follow and understand. Equal parts educational, and terrifying. But also, calming to known this virus better :D
great Presentation is there a lis of cited literature beside the slide?
@ 14:15
cannot we strick or restrict the action of conserved fusion part of spikes.(shown in purple).as that is non variable and can be dealt by pharmaceutical interference as it has very vital role in entering the cell structure..
just suggesting and I am total layman for genomic
Was boring in the beginning, and then it turned insightful, educational (not only regarding Coronaviruses), interesting and some other adjectives too.
Glad I found such a high level source on RUclips.
@ Self Censorship....Please keep looking.....
Excellent presentation. Very clear and precise speaking style. Good job!
Does the ACE2 receptor have any enzyme activity during its interaction with the virus or is it just structural?
Excellent lecture having deep understanding of molecular virology
great lecture, but where is the link that the prof. mentioned?
For an hour of lecture, I have learnt a lot of coronaviruses and it is very useful. Thank you so much, Professor.
Nice lecture. Steady flow of information. Well organized. Thanks.
best video seen so far on SARS-CoV-19!
mario amendola Check This week in Virology-also excellent
It's called SARS-CoV-2. The disease is COVID-19.
Infinity Salinity correct 30 kB in size RNA single strand positive..
Thank you Dr. Glaunsinger.
great video, thanks
That's an awesome lecture... Thanks a ton!
really thank you for sharing all this knowledge!!!
Very nice compilation of current knowledge of SARS-COV-2 molecular virology. As a retired biomedical scientist, makes me want to put on a white coat and get back at the bench.
Hi I wanted to ask about a proper enzymatic assay for RNA Dependent RNA polymerase activity that we can use in the lab for testing antiviral activity and it has no radioactive thing in it
As a layman, I want to thank you for a very interesting talk on the virus' mechanisms. I have probably 20% or less of the prerequisite knowledge to fully understand the contents, so, though I undoubtedly missed a number of nuances, I feel I could explain the top tier of info you provided. Thank you for making an accessible talk without paring it back too far.
I'm stunned at the tactics of the virus at each stage of it's cycle, and,frankly, equally stunned that we can collectively discover and understand them. Good job to all involved. Thank you, and keep up the good work!
Thanks for sharing such an interesting lecture.
Incredibly lecture, thanks for it
thank you for the great, detailed video
very easy to understand and lots covered
Thank you for this informative lecture 🙏🏻🌷
what is the imporatnce of biochemistry in covid-19? you can give any information about this..i m very thankful to u
Great job and please keep up the great work.
A great lecture!!
Quiet complicated!!
Great lecture, many thanks!
unnerving to hear such a knowledgeable calming voice describe this horrific time. our current global sadness, pain, and death deserves all the transparency labs and specialists can honestly pour out of their brain.
Please say a size of molecula of coronavirus? Thank you!