How to purify microbial and host DNA from stool samples

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  • Опубликовано: 13 сен 2024
  • Learn how to isolate microbial DNA that accurately reflects the diverse microbes in the community sampled. This video will provide an outline of stool microbial DNA isolation, plus some tips and tricks. In addition to stool, the PureLink™ Microbiome DNA Purification Kit can be used to isolate DNA from urine, saliva, swabs, transport media, microbial culture, and soil.
    The online manual describes the protocol in detail: tools.thermofi...
    For more information on PureLink™ Microbiome DNA Purification Kit, visit: thermofisher.com/microbiome

Комментарии • 10

  • @MsRabiabatul
    @MsRabiabatul 2 года назад +1

    Can I use this kit to isolate protozoan sppecies of eimeria

  • @laurengregory5843
    @laurengregory5843 4 года назад

    I’m looking to be a part of an undergraduate research program to, in lamen’s terms, collect and analyze otter poo. I have never done anything of the sort so I wanted to research how poo was analyzed.... I’m shocked and amazed by how much work goes into it

    • @thermofisher
      @thermofisher  4 года назад +3

      Science takes us to many obscure places...many of which require washing your hands.

  • @MrFilu13
    @MrFilu13 Год назад

    Hi plz clearify
    the equality of extracted stool DNA is good enough to go for metagenoomic study??
    That 260/280 raito we'll get?

  • @shanefitzgerald9339
    @shanefitzgerald9339 4 года назад

    Great video thank you! Is the protocol the exact same for swab samples?

    • @thermofisher
      @thermofisher  4 года назад

      Hi Shane. Thank you for your feedback and question.
      The protocol for swab samples is available on the product page. www.thermofisher.com/order/catalog/product/A29790?SID=srch-hj-A29790#/A29790?SID=srch-hj-A29790.
      For additional questions, please contact our technical support team at thermofisher.com/askaquestion.
      Thank you!

  • @sherines1918
    @sherines1918 3 года назад

    Is the protocol the same for oral samples ?

  • @lilianjose5907
    @lilianjose5907 4 года назад

    Is the method different for the extraction of microbial and host DNA? Is there really an obvious result between the two DNA?

    • @lilianjose5907
      @lilianjose5907 4 года назад

      @@thermofisher Thank you!

    • @thermofisher
      @thermofisher  4 года назад

      Thank you for your question.
      There isn’t a way to distinguish between microbial and host DNA but if you do have questions about one of our DNA extraction kits, please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!