Isolation of Genomic DNA from Plant Tissue

[@RajashreeRJK]

I'm proud to say that its my Department 🥰🙌🏻our Mam explains really well ...
accuracy matters 📈 💯 Smoothest outcome of the practical
looking forward for further videos🙏🏻

[@ratnakalita6471]

Thank you very much

[@user-xz6ci2ty9k]

Biotechnologist🔥

[@mandipkhanal10]

Wow nice and clear explanation
Thanks Ma'am

[@upscmotivation5156]

This is a very helpful video mam, i an doing phd from CCSHAU, hisar. Thank u so much

[@priyabhorali7736]

Best wishes

[@nemkumarsahu8050]

Very clear explanation mam ji 🙏🙏🙏🙏

[@easylearningzone9783]

Thank you ma'am
Very well explanation

[@siya-f7z]

Really amazing work and clear explanation

[@swapnaswagatikamishra6494]

Thank you so much for such a crystal clear explanation. Really helpful!

[@ratnakalita6471]

thank you

[@bhagyalakheephukan9585]

Thank you ma'am.... For clearing the concept...

[@gurleenvlogs6731]

Amazing explanation

[@ratnakalita6471]

thank you

[@raviranjankumar-yp6cx]

Best ever explained ma`am

[@ratnakalita6471]

thank you

[@yuvanthim7589]

Thank you mam.its very useful for us

[@nemkumarsahu8050]

Thank you so much mam ji 🙏🙏🙏🙏

[@ShortHub2001]

Tnq so much this was truly helpful❤

[@saneha7020]

Great video

[@beirachhithabohia9792]

How much mercaptoethanol should be added?

[@pratimausendi3703]

A very helpful video mam but in case of chilli crop follow same protocols or something different mam ?

[@priyabhorali7736]

You can follow this protocol for chilli

[@krishikrishna3694]

I have added rnase after dissolving the dna in NFW and then again washed with ethanol re airdried and dissolved in NFW but my dna is lost. No concentration in nanodrop. Why does it happened?

[@priyabhorali7736]

If you want to purify DNA from RNase, then do another round of purification of the dissolved DNA with PCI, and follow the rest of the steps. However this step results in loss of DNA. So, at the end you should dissolve it in a lesser amount of nfw or TE buffer (e.g. 30-50 ul) depending upon how good was the qty that you recovered initially

[@tarangabantawa-zb1ie]

Thank you for clear guidelines

[@ratnakalita6471]

🙂

[@spinninlikeAngelG]

thank You

[@mutazkhalid7444]

how much we have to add phenol:chloroform:isomyl alcohol? and how much chloroform: isoamyl alcohol in the purification steps

[@priyabhorali7736]

The same volume as used for the extraction buffer. If you add 700 ul of buffer, add the same amount of PCI or CI for purification.

[@nirmalpatel5249]

Hello mem
Thenk you so much for the crystal clear method demonstration.
I have question regarding this. We can crushing in nitrogen then how much time we store these material??? Like one month or 15 days?
Any idea about this.

[@priyabhorali7736]

Sorry we haven't checked how long the crushed sample can be stored. Can be stored for a few days but in ultra low temperature.

[@MedalYTLive]

❤

[@hpatel4708]

Result?

[@aminasaeed4386]

thank for sharing

[@Shivanichaudhary00]

Thnx mam

[@anushkameena1216]

👍

[@HarmonyLover-bo8wm]

Mam can we done it by without centrifuge...?

[@priyabhorali7736]

No

[@devaorios5793]

G Shit