Reverse transcription PCR - RT PCR procedure

Reverse transcription PCR - This lecture explains about the principle of reverse transcriptase polymerase chain reaction also known as RT PCR. RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation. Watch this video lecture to understand the mechanism behind the reverse transcriptase PCR to amplify target RNA sequence by reverse transcribing it into complementary DNA.
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Sir I was suffering with a confusion in this reverse transcription all thanks to you I got all my concepts cleared thankyou sir ❤️

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Sir I have question regarding an experiment utilising qRT-PCR: what does it mean to normalise a particular gene (in RNA form) into a GAPDH mRNA and further to cell numbers?

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Sir, can you make video on Polymerase Spiral Reaction (PSR) or LAMP PCR..? As there is no video on this topic on you tube and I hope I will get on this platform only .
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would we then determine the amount of dna produced by analzying its quantity on a spectrophotometer and then from that deducing the quantitry of rna present

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[@sanskardauneria1070]

Sir question**there is uracil in place of thymine as a base pair in RNA but in DNA we have thymine so from where did the thymine come from two RNA that have uracil in reverse transcript ion

[@lebronprasad2632]

Can u provide video on mini preparations and purification of plasmid dna

[@roseb2105]

why cant one just use 37 degree temperature even with multiple repeats when pcr process happens doesnt it act on ( multiple strands and sequences at the same time i dont understand the thing about higher temperatures with repeats?

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Glad you liked it

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Is reverse trancriptase pcr the same as cdna library? Which then is used for pcr? Im sooo confused

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one question:
how will you know whether a homolog of a known bacterial gene is present in an unknown bacterial genome?
confusion between PCR and Souther Blotting

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Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me

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If I use two type of primer will the cDNA be one strand or multiple fragments?

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Dear sir can you post a video to explain RT-LAMP.

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Another great video. Thanks. I am about to start doing RNA purification and cDNA creation so good to get a theory primer first:)

[@shomusbiologyofficial]

Thank you

[@mallikanagamanickam5535]

Sir u mentioned tat rtase won't survive at higher temperature Tan 37 rite for linearising of rna we r heating it to 70 so won't tat denature tere itself

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THANK YOU SO MUCH

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Please make a video on 5' and 3' RACE

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Can you please add the sources/cite that use?

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Good sir.
Please do video on Molecular markers and QTL mapping

[@shomusbiologyofficial]

Sure

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Can someone explain the difference between the Random primer and oligodT because he said both of them can bind to any place and start replication.

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No the oligo dT primers will bind to the poly A tail ,they are not random.....whereas the random primers bind to the random sequence of the m RNA

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[@shreyasuresh9429]

Is Rapid Amplification of cDNA Ends (RACE) a type of Reverse Transcriptase PCR?

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Sir what is the time required for the overall process?