Histology Bone marrow smear : Shotgun Histology

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Histology Bone marrow smear
Component Cells of a Bone Marrow Smear
While the peripheral blood smear indicates the status of mature blood cells, the bone marrow smear can be used to assess the process of hematopoiesis, or blood cell formation.
Active bone marrow appears highly cellular. The majority of the developing cells will become erythrocytes, which confer a red color to the marrow. For this reason, active bone marrow is also known as red bone marrow. Over time, the marrow becomes less active and its fat content increases. It is then referred to as yellow bone marrow.
Once again, there are several important characteristics to take into account when viewing a bone marrow smear. These include:
Size of the cell
Cytoplasm to nucleus volume ratio
Shape of the nucleus
Degree of chromatin condensation
Presence or absence of nucleoli
Cytoplasmic staining
Presence of cytoplasmic granules
The blast cell is a pluripotent stem cell from which erythrocytes, granulocytes, and lymphocytes originate. Erythrocytes develop from erythryoblasts, granulocytes from myeloblasts, and lymphocytes from lymphoblasts. These cells, however, all appear identical - they are large with round or ovoid nuclei, a distinct nuclear membrane, visible nucleoli, and an abundant blue cytoplasm. As the blast cells differentiate, the resultant cells can be assigned to a particular cell line.
Erythropoiesis is the development of red blood cells. There are several recognizable steps in this lineage:
The erythroblast develops into a proerythroblast, which is only slightly smaller than the blast, but has a more basophilic cytoplasm.
The basophilic erythroblast forms when the proerythroblast loses its nucleolus. These cells are much smaller than the blast cells and have an intensely basophilic cytoplasm that results from the accumulation of ribosomes.
The polychromatophilic erythroblast has a darkly staining nucleus and its cytoplasm stains a grayish-green color due to the accumulation of hemoglobin.
In the orthochromatic erythroblast, or normoblast, the nucleus becomes smaller and darker and the cytoplasm becomes pinker. Nuclear expulsion occurs at the end of this stage through an asymmetric division of the orthochromatic erythroblast. The portion that contains the cytoplasm and organelles becomes the reticulocyte, while the portion containing the nucleus is destroyed by macrophages.
The reticulocyte contains cytoplasm, cytoplasmic organelles, and many ribosomes. It is released from the bone marrow and develops into a mature erythrocyte after spending 1 to 2 days in the peripheral blood.
Granulopoiesis is the process by which white blood cells develop. The myeloid series has the most characteristic cell lineage:
The myeloblast differentiates into a promyelocyte that becomes irreversibly committed to the neutrophilic cell line. This cell is large, with a large round nucleus, prominent nucleoli, and purple azurophilic granules. These granules are primary, nonspecific granules. Promyelocytes also give rise to eosinophils and basophils
The myelocyte stage is characterized by the production of secondary, specific granules. Myelocytes can vary in cell size and nuclear shape. They contain both the purple staining azurophilic granules and lilac staining specific granules. As they develop, they decrease in size, their nucleus becomes indented, and there is a shift toward more specific granules. There is also a reduction in the number of organelles, which results in decreased basophilia of the cytoplasm.
The metamyelocyte has a flattened nucleus with condense chromatin.
The band cell has a horseshoe-shaped nucleus that is "immature." As development continues, it will mature into a segmented nucleus with multiple lobes. It will then be a mature neutrophil.
Eosinophils and basophils undergo sequential stages of differentiation in a very similar manner to those of neutrophils. Their specific granules are also produced during the myelocyte stage.
The platelet lineage is similar. Large, multilobed promegakaryocytes develop into megakaryocytes, which are the largest cells of the bone marrow (30 to 40 microns). Platelets form through the segmentation of these cells.
Monocytes develop from promonocytes and lymphocytes develop from prolymphocytes. These elements are difficult to distinguish in normal bone marrow smears.
#histologyofbonemarrowsmear #bonemarrowsmear #bonemarrowsmearhistology #shotgunhistology #histology

[@sameerapinky2995]

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[@marceldagli4229]

Great content!

[@suresh9384]

Thank you. This is easy to follow.

[@doctorbhanuprakash]

You are welcome!

[@jagratibulchandani4852]

Geimsa stain used
White round area area of fat ( lobular area )
Megakaryocytes distinctly larger then other cell .
Round nucleus blue cytoplasm precursor cell of rbc ( normoblast )
As matures nucleus smaller and darker

[@marionall]

Thanks for sharing!!!:)

[@Hira-gt8cy]

Sir can u help me about
Quantity the bone marrow lineage in histology section

[@Hira-gt8cy]

It's not for me
I wanna know about this for my friend for helping it's
So she is dr. Ss

[@Hira-gt8cy]

Hello

[@qasqhtan]

❤