Internal Standards

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  • Опубликовано: 5 ноя 2024

Комментарии • 2

  • @francescak6659
    @francescak6659 4 года назад

    Thank you Dr. Mabbott! This was extremely helpful for me as i was trying to solve a similar question which is the following:
    The analgesic ointment content of camphor is determined by GC as follows: Prepare a standard solution by transferring 45.2 mg camphor and 2 mL of 6 mg / mL internal standard solution (a terpene compound) into a 25 mL volumetric flask and diluting to the mark with CCl4. . A 2 µL volume injection of the standard solution in the chromatographic column gave 67.3 and 19.8 area values ​​for camphor and the internal standard, respectively. A sample of 53.6 mg of analgesic ointment was placed in a conical flask, 10 mL of CCL4 was added followed by stirring, heating to 50 ° C (to facilitate camphor extraction), cooling to room temperature, filtration, filtering with 10 mL CC4. , add 2 mL of 6 mg / mL internal standard solution and finally dilute to 25 mL with CCl4. Injection of 2 µL of this solution in the column gave 24.9 area values ​​for camphor and 13.5 for internal standard. What is the% w / w ointment content of camphor?
    I managed at last, after watching your video, to find a result of 45,5% . I hope i have it correct!!!!

  • @2hardenthemind
    @2hardenthemind 8 лет назад

    Thank you Dr. Mabbbott! I thought your voice sounded familiar. I met you when we needed assistance in fixing the capillary electrophoresis system, thanks for that. Now tomorrows lab for quantitative analysis will be able to obtain some data! I appreciate these online tutorials, you've gained a subscriber.
    -Zak