Hello Nicholas. Sorry, I could not share the video on capturing SAED with ceta camera (our institute is still closed). But I am glad you figured out and this way is even better than I follow. Thank you very much.
Hey Nicholas, thanks for such great videos! Maybe you have explained this before and I've missed it, but how would make the center spot little (same size as the others) if you go into diffraction mode, and is big? I am working with a C1 of 2000 and C2 of 100, with SA of 10. Thanks!
Hi Alan: make sure your SA aperture is retracted when focusing the DP. Assuming you are properly focused on the back focal plane of the OL post-field, you then want to use the C2 lens to focus the spots to as small as possible. Once you do this, your beam is now parallel and you can then go back to image mode (but do not make any further adjustments to C2). If you want, you may then insert an SA aperture to field limit the contribution to the DP. I hope this helps and thank you for your support.
Hi Jillian: doing diffraction from single NPs can be very tricky due to the poor signal (like you've already noticed), a better option may be to take a high quality HR-TEM image and then compute the FFT rather than try to collect an actual SADP. The other option is to perform CBED in microprobe mode instead, which should greatly improve the signal in the DP because basically all of the beam (depending the particle and beam size, of course) will be interacting with the particle. This could also be done as part of a 4D-STEM experiment if you have a group of different particles, so the CBED patterns from different particles could be isolated (spatially-resolved). If you still have questions about this, please send me an email.
Thank you for sharing such helpful videos. It really helps a lot to practice TEM
Thank you
You're welcome.
Hello Nicholas. Sorry, I could not share the video on capturing SAED with ceta camera (our institute is still closed). But I am glad you figured out and this way is even better than I follow. Thank you very much.
Hey Nicholas, thanks for such great videos!
Maybe you have explained this before and I've missed it, but how would make the center spot little (same size as the others) if you go into diffraction mode, and is big? I am working with a C1 of 2000 and C2 of 100, with SA of 10. Thanks!
Hi Alan: make sure your SA aperture is retracted when focusing the DP. Assuming you are properly focused on the back focal plane of the OL post-field, you then want to use the C2 lens to focus the spots to as small as possible. Once you do this, your beam is now parallel and you can then go back to image mode (but do not make any further adjustments to C2). If you want, you may then insert an SA aperture to field limit the contribution to the DP. I hope this helps and thank you for your support.
@@NicholasRudawski Thank you for the advise! I'll give it a try this week.
Hi Nick. If you have a sample producing a more faint diffraction pattern (like single nanoparticles), what settings would you adjust?
Hi Jillian: doing diffraction from single NPs can be very tricky due to the poor signal (like you've already noticed), a better option may be to take a high quality HR-TEM image and then compute the FFT rather than try to collect an actual SADP. The other option is to perform CBED in microprobe mode instead, which should greatly improve the signal in the DP because basically all of the beam (depending the particle and beam size, of course) will be interacting with the particle. This could also be done as part of a 4D-STEM experiment if you have a group of different particles, so the CBED patterns from different particles could be isolated (spatially-resolved). If you still have questions about this, please send me an email.
I have a question on the rotation centering.
Is it the same with pushing wobbler button and then minimizing the motion?