Identify Doublets using scDblFinder in scRNA-seq dataset

[@usamahussein1254]

Thank you very much for informative tutorial. You helped us a lot.❤

[@santosh6148]

Thanks for the informative videos.

[@asshimul1168]

Thank you for this tut❤️❤️

[@苏秋羊]

Professor, thanks for your video. I have one question, could i use scDblfinder with mergerd data? Thanks.

[@Collection_of_online_tutorials]

yes

[@Collection_of_online_tutorials]

### Identify doublets using scDblFinder
library(Seurat)
library(tidyverse)
suppressPackageStartupMessages(library(scDblFinder))
set.seed(123)
# 1. Create Seurat Objects
NML1

[@saleenayounus7797]

Dear professor could you make a video for stemness features identification

[@VivinaPuthur]

Sir, in the creation of Seurat object step, why did you give the condition min.cells = 3 ? Is 3 cells a default threshold value ? Also how to fix the threshold value of nFeature_RNA and nCount_RNA ?

[@Collection_of_online_tutorials]

you can change it according to your data, default means you don't need to set it

[@VivinaPuthur]

Sir, how to fix the threshold value in quality control filtering according to our data ?

[@Collection_of_online_tutorials]

they are determined by the violin plot