You are supposed to draw the loop out of the urine in a way the reduces overcharging the loop (seen as bulging of urine out of the loop) and to make sure the loop contains no bubbles before streaking and then redip the loop the same way after streaking the blood agar to inoculate the MacConkie agar. otherwise, you’ll have two extremely different volumes of urine used on the two plates. Also, avoid touching the edge of the media where it contacts the plastic plate.
I was thinking the same thing as I watched her streak the plate with the same loop. Even though the loop touched a urine plate, I would have used a new loop.
Thank you so much for the videos! I’m glad after taking microbiology lab; it makes so much sense with the videos you are showing! I’m learning a lot and feel prepared by you since this is what my future consists of in this field!!
Even if the colony count is typically taken from the BAP, it's better practice to re-immerse your calibrated loop with every successive plate you streak.
never use a calibrated loop method before during uni (i studied microbiology but we never use human specimen) so i never knew it's existence until i work for a polytechnic of health. a student went to me and asked about how to interpret the result and there i was, never knew that something like this even exist 🤣🤣🤣 thank you for the video, this really help me visual the result
Well I think the streaking is not the typical you see in the clinical lab or shown on school in my case but is very informative. I have encountered lots of different techniques
Draw the edge (not face) of the loop against the cup when coming out of the urine (and away from any bubbles). This helps assure correct loading and reduces bubbles in the loop. Kind of like drawing a urine dipstick along the edge of the cup to help reduce overloading the various pads.
This a very informative video but it overlooks that BAP as primary culture media is inappropriate; presence of Proteus complicates colony counting. Instead, CLED agar should be used.
Aren't you supposed to avoid spreading the specimen to the outer perimeter? Your loop keeps touching the plastic perimeter. The outer perimeter is where airborne contaminants is most likely to be located.
im really thankful to you for making those videos 🙏🏻. i only have one question. im really confused on which type of media we use for each sample (like for urine, we use mac and blood and stool we use selenite... etc). do u have any guide/book that can help me memorize which media to use for each sample? i would appreciate it
Thank you for your support! Not currently. I do have many projects in the works, this being one of them. The main thing to remember is that you're using media that will isolate common pathogens from that body site (specimen). Blood Agar Plate should grow most aerobes and facultative anaerobes. Chocolate will grow fastidious bacteria, such as Haemophilus and Neisseria, but most Haemophilus only grow on CHOC. MacConkey will grow the Gram Negative bacilli such as the Family Enterobacteriaecae (from the GI tract) and Non-fermenters (that get mistaken for Enterobacteriaecae). The anaerobic set-up would be used anytime anaerobes are expected to be the pathogen (aspirates) and should be plated along with the full aerobic primary set-up (BAP, CHOC, MAC).
This is the problem with cultures. Multiple bacteria is considered to be contamination. Please teach your students the correct info - multi species infections are very much NOT contamination and labeling it as that on cultures prevents women from getting proper treatment. Luckily, I found a urologist who gets this (very few do) and finally identified the 3 bacteria in my infection using a much better test called Pathnostics.
So true. I've been suffering for 7+years with monthly UTI'S 😫 and I didnt know I was and it was so painful and hard to urinate and bleeding. I have to take meds every month for my UTI'S. One day I had one so bad and I was starting to bleed and had to see a different doctor and they had marked my specimen as contaminated and I was like no that can not be because I am actually bleeding and the pain is horrible and I have them every month. They re did the urine culture and this time it wasn't marked contaminated so they gave me meds
We work entirely different. How do you find " clean" colonies when you are striking like this? We do 3x striking with 3 Loops and we use the same loop for the first striking on Mc and blood with no problem. How many different bacteria do you test? When you have 3 ( prxx, ec and Enterococcus for example in same count) do you test or is it contamination? Nice video!!!
You are supposed to draw the loop out of the urine in a way the reduces overcharging the loop (seen as bulging of urine out of the loop) and to make sure the loop contains no bubbles before streaking and then redip the loop the same way after streaking the blood agar to inoculate the MacConkie agar. otherwise, you’ll have two extremely different volumes of urine used on the two plates.
Also, avoid touching the edge of the media where it contacts the plastic plate.
I was thinking the same thing as I watched her streak the plate with the same loop. Even though the loop touched a urine plate, I would have used a new loop.
Thank you so much for the videos! I’m glad after taking microbiology lab; it makes so much sense with the videos you are showing! I’m learning a lot and feel prepared by you since this is what my future consists of in this field!!
So glad you're joining the field! Welcome!
Even if the colony count is typically taken from the BAP, it's better practice to re-immerse your calibrated loop with every successive plate you streak.
Happy Lab Week! Thanks for the video!
I am from pakistan and student of bs microbiology. I am too much confused but your vidoes are informative and learn lots of things from them
I love your streaking skills!
never use a calibrated loop method before during uni (i studied microbiology but we never use human specimen) so i never knew it's existence until i work for a polytechnic of health. a student went to me and asked about how to interpret the result and there i was, never knew that something like this even exist 🤣🤣🤣
thank you for the video, this really help me visual the result
Glad to help!
Well I think the streaking is not the typical you see in the clinical lab or shown on school in my case but is very informative. I have encountered lots of different techniques
Thank you so much for this educative video Ma'am
Draw the edge (not face) of the loop against the cup when coming out of the urine (and away from any bubbles). This helps assure correct loading and reduces bubbles in the loop. Kind of like drawing a urine dipstick along the edge of the cup to help reduce overloading the various pads.
Thnkeo thnkeo ma'am,for your efforts ,,,May God bless you ,and may you get success in evey field of life ,,
This a very informative video but it overlooks that BAP as primary culture media is inappropriate; presence of Proteus complicates colony counting. Instead, CLED agar should be used.
I love the Microbiology videos!!! I'm definitely going to recommend these for my new techs. Will you please do one on gram stain interpretation?
Thank you for your support! We are not in Micro this semester but I will do my best to get one done when we are.
@@MedicalLabLadyGill hello how are you?
@@MedicalLabLadyGill see tobbaco mosaic virus leaf disease and coronavirus in microbiological microscope electronic because it
@@MedicalLabLadyGill search in you tube about tobbac mosaic virus leaf disease and coronavirus in
Tobacco leaf disease
Not worried about Proteus swarming all over the BA?
Very informative videos. Keep doing this educational videos. Love them. Thank you very much 💖
Thank you so much for your support! I'm glad you find them helpful! ♡
Aren't you supposed to avoid spreading the specimen to the outer perimeter? Your loop keeps touching the plastic perimeter. The outer perimeter is where airborne contaminants is most likely to be located.
Good work thanks ❤❤
What is the composition of the media for this test ? Agar and what other components ? thanks
which practical microbiology book will you recommend me to read please? I highly appreciate your valuable video.
I would love to work my internship with you 😷🙏🏻
im really thankful to you for making those videos 🙏🏻. i only have one question. im really confused on which type of media we use for each sample (like for urine, we use mac and blood and stool we use selenite... etc). do u have any guide/book that can help me memorize which media to use for each sample? i would appreciate it
Thank you for your support! Not currently. I do have many projects in the works, this being one of them. The main thing to remember is that you're using media that will isolate common pathogens from that body site (specimen). Blood Agar Plate should grow most aerobes and facultative anaerobes. Chocolate will grow fastidious bacteria, such as Haemophilus and Neisseria, but most Haemophilus only grow on CHOC. MacConkey will grow the Gram Negative bacilli such as the Family Enterobacteriaecae (from the GI tract) and Non-fermenters (that get mistaken for Enterobacteriaecae). The anaerobic set-up would be used anytime anaerobes are expected to be the pathogen (aspirates) and should be plated along with the full aerobic primary set-up (BAP, CHOC, MAC).
@@MedicalLabLadyGill pp 0pol look plol00plloooo9o9ooo
@@MedicalLabLadyGill 9œlll9ooollo9
Why u can't Streak on CLED agar bc I always streak urine on CLED only.....when in student life
This is the problem with cultures. Multiple bacteria is considered to be contamination. Please teach your students the correct info - multi species infections are very much NOT contamination and labeling it as that on cultures prevents women from getting proper treatment. Luckily, I found a urologist who gets this (very few do) and finally identified the 3 bacteria in my infection using a much better test called Pathnostics.
How r u please
I face klebssiella penumonia bacteria what I do
@@WorldAffairsभारत1 Please find a good doctor that will treat you with at least 14 days of antibiotics targeted to your infection.
@@aprilsmith8924 yes bro I treat this problem antibiotics.
Thanks for suggesting
@@aprilsmith8924 Bhai antibiotic injection bhi lagane honge 5 days
So true. I've been suffering for 7+years with monthly UTI'S 😫 and I didnt know I was and it was so painful and hard to urinate and bleeding. I have to take meds every month for my UTI'S. One day I had one so bad and I was starting to bleed and had to see a different doctor and they had marked my specimen as contaminated and I was like no that can not be because I am actually bleeding and the pain is horrible and I have them every month. They re did the urine culture and this time it wasn't marked contaminated so they gave me meds
What is this streaking technique called?
Inoculation of agar plates to identify organisms (bacteria) present in the urine sample.
Thank you so much
I learn a lot from your videos 🙌
You're so welcome! Thank you for your support!
Thanks you for sharing
We work entirely different. How do you find " clean" colonies when you are striking like this? We do 3x striking with 3 Loops and we use the same loop for the first striking on Mc and blood with no problem. How many different bacteria do you test? When you have 3 ( prxx, ec and Enterococcus for example in same count) do you test or is it contamination? Nice video!!!
Yeah..but she could have perform sub culture from this one
Why you putting in CO2 incubator
How much temprature of incubator??
Incubator is at body temperature (37°C)
Thank you 😊
Thank you for your support!
is the urine sample you used diluted or undergone any form of pre culture preparation? Thank you for explaining!
Good question! Urine should not be diluted or treated in any way before culture.
Explained very well
What if you test negative for leukocytes and nitrites but bacteria was present and the urine culture was from 25-50000 Cfu/ml of E. coli. Im a male
Should co-relate with symptoms.
wow very informative . love from pakistan
Thank you for your support!
Gnr means
Antimicrobial substances present in microbiology report meaning
Can you make this video in hindi
Great video
Thank you for your support!
Gnr means what?
Gram negative rod also known as Gram negative bacillus
Is there any vaccancy for microbiologist
There are always positions available for medical laboratorians! You will never want for a job!
@@MedicalLabLadyGill position available @your lab?
@@saiyednaved not currently but all our neighboring hospital labs have positions available.
@@MedicalLabLadyGill kindly provide adress or e mail adress so I can apply for the same.
knowledge you share are a true blessing Doc Kham on RUclips .
Am so helped,, am looking for someone to help me with an Incubator
What should i do
Thanks ♡♡
What does GNR mean?
Gram Negative Rod (also known as bacilli)
Mucoid colonies on top klebsiella 8:02
One question from my side
I take plenty of water in a day approx 2. 0 litter
2nd step in antibiotics
🥰🥰🥰🥰🥰🥰💟💗💗💗
Reply me soon
I think it is kleb
Thnkeo thnkeo ma'am,for your efforts ,,,May God bless you ,and may you get success in evey field of life ,,
Gnr means
Gnr means
Gnr means
Gnr means
Gnr means
Gram negative rod
@@creativeman17 thank you