1,000,000x Magnification with Atomic Force Microscope

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  • Опубликовано: 19 июн 2024
  • Today we're looking at Atomic Force Microscopy! I built a "macro-AFM" to demonstrate the principles of an atomic force microscope, then we look at a real AFM (an nGauge AFM from ICSPI) and do a few scans in the shop to see how it works.
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    AFM is a complimentary technique to SEM. Both can provide nanometer resolution images, but their different mechanisms (phyiscal probing vs electron beam) means they have different characteristics. AFM excels in precise heightmaps of the sample, easy scanning (no vacuum, no metal coating) and a diverse range of related techniques. SEM offers high depth-of-field and a very large field of view, which makes it easy to get a broad overview of a sample and then zoom in. Labs often use both in research because they compliment each other's strengths.
    nGauge AFM from ICSPI: www.icspicorp.com/
    Macro-AFM inspiration paper: Amin-Shahidi, Darya, and David Trumper. "Macro-scale atomic force microscope: An experimental platform for teaching precision mechatronics." Mechatronics 31 (2015): 234-242.
    Scans are post processed in Gwyddion (gwyddion.net/) and 3D images rendered in Blender
    0:00 AFM Scans!
    1:26 What is Atomic Force Microscopy?
    3:10 DIY "Macro-AFM" and real AFM
    4:34 Macro-AFM overview
    6:44 Cantilever displacement sensing
    7:30 Scanning on the Macro-AFM
    8:45 Tricky aspects of scanning probe microscopy
    10:20 Topographic result of lattice scan
    10:50 Traditional AFMs are big
    12:34 MEMs scanner on the nGauge
    13:45 Scan: Tungsten Carbide insert
    17:42 Scan: Ablated aluminum
    19:47 Scan: Gage block
    20:52 Conclusion
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Комментарии • 904

  • @dienelt5661
    @dienelt5661 3 года назад +1935

    Shows the results at the beginning of the video : ABSOLUTE LEGEND

    • @annaw.1951
      @annaw.1951 3 года назад +52

      That's actually a really good way to grab attention, especially with results that good I want to know how it was achieved :D

    • @ruiqiangliu3511
      @ruiqiangliu3511 3 года назад +5

      This guy has a PhD degree

    • @shadowknight7584
      @shadowknight7584 3 года назад +3

      I agree

    • @geobot9k
      @geobot9k 3 года назад +12

      That alone got me to sub

    • @aaronnekrin5150
      @aaronnekrin5150 3 года назад +4

      Super absolute legend

  • @MatryxMayhem
    @MatryxMayhem 3 года назад +1128

    The best part. Is that Historically humanity in the past has poked things with sticks to try and understand them. But in the present day we still do, the stick has just become a whole lot cooler.

    • @SpeakerWiggin49
      @SpeakerWiggin49 3 года назад +16

      We never imagined textures could be so quantified. Our ancestors knew nothing of the micro world, they couldn't even conceive of a stick as small as a nanometer and moving so quickly.

    • @tericulbertson4430
      @tericulbertson4430 3 года назад +24

      @@SpeakerWiggin49 that’s not the point, the point is our ancestors poked things with sticks to try and understand them just like we’re doing now with the microscopic world

    • @angrydragonslayer
      @angrydragonslayer 3 года назад +3

      @Stellvia Hoenheim ..... Pls stop.

    • @jeffmorris9893
      @jeffmorris9893 2 года назад +9

      Come here. We are experimenting on people.
      -How do you experiment on people?
      We stick things in them.
      - Are you from the Biology Lab?
      What's a bilogee lab?

    • @xX1GuNNy1Xx
      @xX1GuNNy1Xx 2 года назад +1

      They are a lot cooler for sure!!! And much much much smaller 😂

  • @RememberingMaryEvely
    @RememberingMaryEvely 2 года назад +104

    I've been retired some 12 years now, but back in the '90s I lead a team to select and purchase an AFM. I then supported it (and its Windows 3.1 interface) and trained users. Your model is excellent.

    • @sdrc92126
      @sdrc92126 Год назад

      Back in the 90's I was working with one of my profs building these. stm actually. iirc, there were doubts as to whether they were actually showing the images they said they were...

    • @nicholaswood7704
      @nicholaswood7704 4 месяца назад

      Hey we're waiting for somebody to prove that about our own perceptions? Are we really hearing what we think were hearing are we really seeing what we think we're seeing how do you know?

  • @t0k4m4k7
    @t0k4m4k7 3 года назад +467

    Can we talk about how he literally built a scanning microscope for demonstration purposes?

    • @deepspacemachines
      @deepspacemachines 3 года назад +46

      macroscope :o

    • @t0k4m4k7
      @t0k4m4k7 3 года назад +7

      @@deepspacemachines lol true

    • @x1Conan3Spartan0x
      @x1Conan3Spartan0x 3 года назад +10

      This is a completely different lifestyle of focus. I thought my KD in Cod was good... this man is helping solve wonders of the world.

    • @t0k4m4k7
      @t0k4m4k7 2 года назад

      @d3adsoulja i meant the macroscopic one

    • @WmSrite-pi8ck
      @WmSrite-pi8ck 2 года назад +4

      I don't think it is a "scanning microscope." I think it's actually a "probing macroscope."

  • @p41nk1ller2
    @p41nk1ller2 3 года назад +121

    Amazing! As a technician in a physical lab i applaud you for the great explanation, and the macroscale AFM makes it so much clearer for everyone!

    • @p41nk1ller2
      @p41nk1ller2 3 года назад +2

      @Stellvia Hoenheim I have basic knowledge of AFM, i am not an expert. (I learned new things)
      My thanks is for the time/effort spend on the research, explanation and rebuilding a 3d printer to AFM to teach people the principle.

  • @satrioarif1797
    @satrioarif1797 3 года назад +343

    Love this so much, its like the most basic nature of humanity.
    "What the fuck is this? lets poke with a stick" thoughts stand the test of time to the fricking atom

    • @199NickYT
      @199NickYT 3 года назад +24

      YES!! That and "let's smash these two things together to see what flies out"

    • @theman13532
      @theman13532 3 года назад +18

      @@199NickYT you forgot "lets burn these things to see what happens"

    • @199NickYT
      @199NickYT 3 года назад +3

      ​@@theman13532 Or even "let's poke it with a (nanoscopic) stick"

  • @iain3713
    @iain3713 3 года назад +168

    Holy shit that pcb is crazy, those little flexures are really cool

    • @BreakingTaps
      @BreakingTaps  3 года назад +30

      Right?! Miracle of engineering, it's wild that these things are possible at all.

    • @claypotts2334
      @claypotts2334 3 года назад +1

      @Stellvia Hoenheim Stellvia of the Universe

  • @faxezu
    @faxezu 3 года назад +25

    As a PhD Student in MEMS technology the chip a 7:18 just put me in awe.
    I know how complex the mechanics of the "simple actuation" I want to achieve is. This thing with x/y/z controlled motion is just beyond and stunning to see in almost exclusive silicon.
    Edit: 13:06 blew me away even more! One can still see the point the the "arm" was etched free from the surface, amazing!

    • @BreakingTaps
      @BreakingTaps  3 года назад +5

      Pretty wild, right? It's amazing what MEMS engineering can do these days! IIRC, the actuators are thermal bimorph actuators (instead of electrostatic comb, which is what I assumed at first), which is just super cool :)

  • @Justin-dv7ul
    @Justin-dv7ul 3 года назад +51

    Atomic force microscopy is one of my favorite microscopy techniques, just because it can see down to nanometer resolution, give quantitative data in the Z direction, and the sample does not needs to be in a vacuum. compared to scanning electron microscopy, which requires the sample to be in vacuum, does not see in 3d or give quantitive data in the Z direction.
    Atomic force microscopes are just so cool

    • @absolute___zero
      @absolute___zero 2 года назад +5

      also scanning electron microscope damages the sample because the electrons (since they are accelerated to a somewhat high speed) can hit the electrons in the atoms of the sample itself and affect the valence bonding in the chemical elements under inspection. So, if you making some custom integrated circuit for a client, and you want to verify if it is ok (quality test), you can't use electron microscope since it will introduce defects in the product

    • @Justin-dv7ul
      @Justin-dv7ul 2 года назад

      @@absolute___zero That’s true too

  • @andrewphillip8432
    @andrewphillip8432 3 года назад +92

    It’s really incredible we get to have complete MEMS AFMs in 2021. Seems like this came a bit early

    • @blahblahblahblah2933
      @blahblahblahblah2933 3 года назад +6

      Nah, 2021 owes us.

    • @ExtantFrodo2
      @ExtantFrodo2 3 года назад +11

      2021? We should have halfway mature universal assemblers.

    • @andrewphillip8432
      @andrewphillip8432 3 года назад +6

      @@ExtantFrodo2 idk man transistors aren’t even 100 years old yet. Micro fabrication is still very new

    • @ExtantFrodo2
      @ExtantFrodo2 3 года назад +4

      @@andrewphillip8432 Present day technology is sufficient for individuals to build personal spacecraft as well as orbiting habitats and factories with all necessary life support systems and communications. Yet almost no one understands the power and capabilities of the programmable femto-second quantum cascade laser array which can not only implement catalytic chemical restructuring on demand but molecular positioning and orientation as well for nano-assembly.

    • @bottlekruiser
      @bottlekruiser 3 года назад

      @@ExtantFrodo2 Personal spacecraft?

  • @u_t2347
    @u_t2347 3 года назад +10

    I hear on good authority that "Tapping on atoms with a very sharp stick" is also a highly technical term.
    Great video, easy enough to follow even though I had no idea about your field. Thanks for sharing.

  • @Alexander_Sannikov
    @Alexander_Sannikov 3 года назад +111

    what i really like about your channel right now is that while it hasn't exploded yet, you have enough time to reply to semi-sensible comments that we leave, which i'm pretty sure will not be the case once it takes off :)

    • @BreakingTaps
      @BreakingTaps  3 года назад +38

      Well, if the channel ever does take off in a big way, I'll still try. I like interacting with folks in the comments, I've learned a ton that way! There are _a lot_ of super knowledgeable folks here :)

    • @danielguy3581
      @danielguy3581 2 года назад +1

      @@BreakingTaps MEMS technology is an Ukrainian conspiracy to inject mind-controlling chips into our [1/286]

    • @1SmokedTurkey1
      @1SmokedTurkey1 2 года назад +1

      @@danielguy3581 Correct but it isn't Ukrainian. It was created by the illuminati lizard overlords that [1/274]

    • @Ygr3ku
      @Ygr3ku 2 года назад +5

      @@1SmokedTurkey1 Me, not knowing what you guys are talking about at the end of your comments [1/404]

  • @BreakingTaps
    @BreakingTaps  3 года назад +22

    *Addendum*
    - More footage of the probe scanning here: ruclips.net/video/m0UK7LVSZ8g/видео.html
    - If I mispoke, leave a comment and I'll add to this addendum! I'm new to AFM :)
    - Sorry for the "glow". Some poor life choices were made while filming (fogger, for cInEmAtIc haze) and made my life hell in editing. Lessons were learned 🙃
    - There are _many_ types of scanning probe techniques, I'm only describing a very small handful of techniques for topographic information. I might cover other techniques in the future, there are dozens! There are equally many variations of topographic AFM itself, and each manufacturer has their own special sauce, so my comments are just general statements :)
    - Scans were post-processed in Gwyddion, and the 3D animations done in Blender
    - The Macro-AFM architecture is: arduino driving voice coil and measuring back-EMF, a grbl controller handling stepper motors, Rust program talking to both of those and providing a browser-based UI
    - I should have elaborated on spatial resolution more: the final resolution you get is a combination of tip radius and surface geometry. A wide tip (100nm) can still get you high precision (few nm) spatial resolution if the surface is very flat and the features are not high aspect ratio. But high aspect ratio like nanoparticles or trenches will require a sharper tip that itself has a high aspect ratio, so that the tip can access the internal geometry. So spatial resolution is variable depending on what tips you load and what the sample looks like
    - Gage block is a cheapo import from Shars, so I'm not sure if this is representative of precision ground surfaces in general, or just cheaply ground ones :)

    • @MichaelWatersJ
      @MichaelWatersJ 3 года назад +1

      Nice Blender work! Any reason you stuck with the classic yet boring AFM colormap?

    • @BreakingTaps
      @BreakingTaps  3 года назад +2

      @@MichaelWatersJ No particular reason :) Any suggestions for better color schemes? Definitely new to color maps in general, not sure what the best strategy is

    • @brettkent9851
      @brettkent9851 3 года назад +1

      JET map for the win

    • @LanceThumping
      @LanceThumping 3 года назад

      Do you think it'd be possible to combine this with something like the Open Flexture Stage to slightly move the object over to rescan and expand the area?
      It doesn't need to do this super accurately as long as there is some overlap since that could be used to stitch the scans together automatically.

    • @mateijordache1952
      @mateijordache1952 3 года назад +1

      Awesome video! Only thing to note is that there actually are quite a lot of modes that use contact mode as the base! It does dull the tip more, but with a proper calibration you can limit the forces applied to the surface. Some interesting contact mode applications are like conductive AFM (CAFM), piezo force (PFM), scanning capacitance (SCM) and more!

  • @eVITORIOe
    @eVITORIOe 3 года назад +59

    try it in a CD, we should be able to see the data in the grooves.

    • @pawesomepal7827
      @pawesomepal7827 2 года назад +7

      you can see that with a regular microscope

    • @milothedestroyerify
      @milothedestroyerify 2 года назад +10

      Cds are covered in plastic, you can't get to the pits that are in the foil.

    • @RaduOleniuc
      @RaduOleniuc 2 года назад +3

      @@milothedestroyerify you can remove the foil.

  • @23chaos23
    @23chaos23 3 года назад +42

    DUDE! you have the best toys, so jelly. Ill just go back to my resin printing corner and cry now. lol

    • @reyariass
      @reyariass 3 года назад +2

      @Stellvia Hoenheim Will pocket change do? I have about 3.50

  • @ugurunver2403
    @ugurunver2403 2 года назад +7

    That's basically a vinyl record player in atomic scale. And I liked how that image gauge block surface resembles Mars surface. I mean, i know they just choose to use that color palette for images but i think it's worth to think about the surface detail/mass ratios of both Mars and gauge blocks.

  • @Galeere2
    @Galeere2 3 года назад +23

    This is incredible. Imagine how cheap it could be if it was mass-produced

  • @holemajora598
    @holemajora598 3 года назад +79

    Honestly I can’t think of a single case in my life where I would need this but it’s still cool information.
    Ps: awesome deal, maybe unorthodox but a sweet deal

    • @connerphillips1228
      @connerphillips1228 3 года назад +5

      As a private person there isn't really a need aside from curiosity. But it is absolutely a marvelous research tool.

    • @brertt8350
      @brertt8350 3 года назад +3

      For the sake of science, just the fact that we now know something we didn't before is valuable enough

  • @pyromen321
    @pyromen321 3 года назад +29

    “Glompy” is a perfectly cromulent word

  • @zeropoint5794
    @zeropoint5794 3 года назад +5

    So i've had that project in my mind for a while.
    A geometry scanner to scan complex surfaces in a more or less automated way.
    Mechanically like a 3d printer, but with a probe instead of a hotend.
    Nothing extra fancy, all i want is .5mm of resolution on each axis.
    Guess now i finally have the inspiration for the probe design.
    Great stuff!

  • @Coecoo
    @Coecoo 3 года назад +60

    Everyone: You can't see shit on the atom level cuz it's so small.
    Science: Hold my beer.

    • @sinephase
      @sinephase 3 года назад

      once we're doing lithography in the angstrom range maybe we will?

    • @spartamerican6758
      @spartamerican6758 3 года назад

      Still waiting for those atomic shots....

  • @Ratkill
    @Ratkill 3 года назад +3

    That gauge block surface really put the resolution into perspective for me. Unbelievable. It looks like the surface of mars, not some of machining's most finely surfaced measuring tools.

    • @johndef5075
      @johndef5075 3 года назад +1

      That was amazing. Not what I expected.

  • @MuditGupta89
    @MuditGupta89 3 года назад +3

    that macroscale AFM was amazing! This is quickly becoming one of my favorite channels, unique, very well explained and filmed topics.

  • @z4lper
    @z4lper 3 года назад +13

    This is a very good intro to the SPM world, amazing! One quick note, AFM cantilevers don't have a mirror glued on top, instead they might be coated with a metal, like aluminum and gold for certain applications, like AFM imaging in liquid. Cheers!

    • @BreakingTaps
      @BreakingTaps  3 года назад +4

      Whoops, good point! I was watching a lecture on the origins of STM/AFM and "glued on top" got stuck in my head haha. AFM in liquid is pretty wild!

  • @ShubhamBhushanCC
    @ShubhamBhushanCC 3 года назад +3

    The Macro FM is amazing!!! I did not expect such a great result

    • @BreakingTaps
      @BreakingTaps  3 года назад +2

      Me either! Was honestly shocked how nice it came out, especially after the meh confocal results :)

  • @thepilotmaster777
    @thepilotmaster777 3 года назад +15

    *Pokes atom with*
    *Accidentally splits atom*
    “FUCK”

    • @mierbeuker8148
      @mierbeuker8148 2 года назад +2

      With just one atom, the answer would be, not a lot would happen. In fact with atoms up to the mass of Iron, it actually takes energy to split it. But even with a heavier atom, the amount of energy released from just one atom is so small, my guess is you won't notice anything.
      All of this of course just hypothetically assuming it would even be possible to split an atom, by just tapping on it. In reality, that would be impossible to do with something like this. Atoms are tough little guys, with really strong nuclear forces protecting their integrity.
      In this scenario, I guess it would be akin to trying to open a bank vault, by blowing on it through a straw. 🤣🤣🤣

    • @ColinMacKenzieRobots
      @ColinMacKenzieRobots 2 года назад +1

      @@mierbeuker8148 Wow. You must be a blast at parties. Mansplaining the technical feasibility of every joke. 🤣

    • @mierbeuker8148
      @mierbeuker8148 2 года назад

      @@ColinMacKenzieRobots You wouldn't know, since your gf always chooses one of her other bf's to take her to parties. But hey, at least you get to "respect" her, and give her all your money to call you her bf, right? Chad and Tyrone thanks you for your contribution.

    • @ColinMacKenzieRobots
      @ColinMacKenzieRobots 2 года назад +1

      @@mierbeuker8148 I'm worried about ya man

  • @HuygensOptics
    @HuygensOptics 3 года назад +17

    Great stuff, I really enjoyed this! I was wondering, the samples you showed are pretty flat and parallel. How does it handle large height differences or things like surface tilt?

    • @BreakingTaps
      @BreakingTaps  3 года назад +19

      Depends on how large the height differences are :) So the max Z resolution is 10 microns. If it encounters something larger than that the probe will bottom out/crash, or just start oscillating in free-air no longer touching the surface (like if travelling over a hole). The cantilever is actually pretty soft and flexible so it's unlikely to damage the probe unless you run it into a _really_ large feature which would be noticeable from the microscope. It'll just stop recording data because the oscillation is basically halted. There are also settings which control the size of the oscillation... I usually turn that up when scanning a new sample because it allows you to clear larger objects. Once you're sure the section is "safe" you can turn it down a little, which gives better resolution.
      There's usually some amount of tilt in the scans (due to angle of probe, and the stage not being perfectly parallel) which is corrected when post-processing the data. Different methods to level the image (3 point triangle, intersecting lines, polynomial, etc). If there is extreme tilt it'll be similar to running into large features, at one side of the scan you may bottom out or start scanning air. But OTOH, at a 20um scale even uneven surfaces end up being pretty flat... i was able to scan part of a fly wing for example.

    • @HuygensOptics
      @HuygensOptics 3 года назад +4

      @@BreakingTaps Thanks Zach for this elaborate answer. Sounds like this is a really interesting tool, for example also for layer thickness measurements. I will definitely be checking out this product!

    • @BreakingTaps
      @BreakingTaps  3 года назад +8

      ​@@HuygensOptics No problem, feel free to ping if you have questions! I didn't get into it in the video, but they have different types of probes: sharp DLC tips and less-sharp wedge tips. The wedges are designed specifically for things like thin-film thickness testing since you don't need the high aspect ratio. Apparently last a really long time and are cheaper. I'm going to be doing some thin film testing in the near future, will let you know how it goes :)

  • @hullinstruments
    @hullinstruments 3 года назад +4

    Dude your channel is just awesome. This is going to get so much attention from so many huge RUclipsrs and science lovers alike. I hope you continue down the path of DIY optical tools also. The community needs a well-designed DIY spectrometer, Along with so many other pieces of DIY optical test equipment and scientific apparatus! I think you’re just the man for the job!
    Your last few videos have me so excited about the possibilities!

  • @NvTwist
    @NvTwist 3 года назад +3

    That is one of the coolest tools I’ve seen… & the scans are so quick. 🤯

    • @connerphillips1228
      @connerphillips1228 3 года назад

      For real. I work on a nearly 20 year old system at work and it takes around 8 minutes per scan 😂

  • @CrazyMineCuber
    @CrazyMineCuber 3 года назад +6

    Very cool indeed! I just finished a very similar project at university. We made our own Scanning Tunnelling Microscope! Uses most of the same principles, but instead of tapping on the surface, you move a very sharp probe about an atom away from the surface. Then when a small voltage between the probe and the sample is applied, a magical current appear that is extremely distance sensitive. Our goal was to see atoms, so a micrometer is pretty huge in my brain currently :)

    • @BreakingTaps
      @BreakingTaps  3 года назад +3

      Goodness, now that is definitely brain breaking. Very cool!

    • @_John_P
      @_John_P 3 года назад +2

      @@BreakingTaps It is a bit confusing the difference between "making contact" and "not making contact", as "physical contact" is a remote interaction between fields, so maybe what is meant by "contact" is when the tip is close enough to produce phonons and potentially exchanging atoms, or rearranging them on the surface, hence potentially causing wear, sticking the needle to the surface, cross-contamination and change in topography?

    • @BreakingTaps
      @BreakingTaps  3 года назад +3

      ​@@_John_P Hehe good eye, I definitely glossed over that (well, I recorded a bunch trying to explain, but it was confusing and long so got cut). And looks like I technically mispoke in the final cut as well. So my understanding is that "contact" mode AFM relies on the very-close range repulsive forces between the tip and the surface. I believe this repulsive force starts just a few angstroms above the surface, and is why the cantilever is very soft so as to prevent damaging the surface (or tip) too much as they are strongly shoving on each other at that point. The so-called "non-contact" AFM relies on attractive forces at a longer distance, and measures how the attraction to the surface changes the resonance of the cantilever. I believe non-contact cantilevers are much stiffer to prevent them from being pulled down to the surface, and typically have much more sensitive amplifiers to detect the small attractive force.
      But yeah, you're totally right: all the forces are remote and nothing is _really_ in contact once you get small enough :)

  • @CodingTheSystem
    @CodingTheSystem 3 года назад +1

    This is just absurd. The production quality and depth you go into on your videos never ceases to amaze me. Absolutely stunning.

  • @MasterThief117
    @MasterThief117 3 года назад

    This video is hecking awesome!
    Props to ICSpi to working with you and providing you with this microscope!

  • @amoose136
    @amoose136 3 года назад +55

    It’s a shame they too operate on the “request for quote” pricing model. Look I don’t care if your product cost 1k, 10k, 1M, or 10M just list the damn price, whatever it is.

    • @whuzzzup
      @whuzzzup 3 года назад +10

      Agreed, "request for quote" is the worst thing ever.

    • @caseyjones7404
      @caseyjones7404 3 года назад +3

      Yes I see it, Yes I want to buy it. I need 15. Take my money. Response : we dont have 15, only 12,000 min. Sure, send me the 12000, im going to take 15 out. Send it back on an account of the "picture does not match' cannot be applyed to out exact use.... Request Refund.....

    • @bluekenya4825
      @bluekenya4825 3 года назад +4

      'Request for quote' simply means you might need some therapy before and after you see the price. But no worries. There is always a disruptor around the corner.

    • @jskratnyarlathotep8411
      @jskratnyarlathotep8411 3 года назад +4

      at least what is the scale of price. Is it about $5k? Or $50k?

    • @oni2ink
      @oni2ink 3 года назад +4

      @@jskratnyarlathotep8411 Dunno for this one, but the range of prices seems to be 1k-100k$
      www.afmworkshop.com/afm-products/price-list

  • @party4keeps28
    @party4keeps28 3 года назад +3

    "How cool is that?" 1:04
    *Incredibly Cool*

  • @natalie5947
    @natalie5947 3 года назад +1

    This is the kind of science content I love. Using cool tools to do cool things. Also that was a great use of a scale model. Great content as always. I'm honestly surprised you don't have like 300k subs already.

  • @stephenpuryear
    @stephenpuryear 3 года назад

    Thanks for your transparency. It improves the rest of this very cool video. Looks like a lot of fun!

  • @danielabbey7726
    @danielabbey7726 3 года назад +8

    Wow, this is amazing stuff! Is AFM only for examining rigid materials, or would it also work on cells, viruses, etc?

    • @BreakingTaps
      @BreakingTaps  3 года назад +23

      AFM can do cells, proteins, DNA, etc! The cantilever is actually very "soft" (although the tip is quite hard) so it will happily scan other soft things like cells or polymers. Mine can only do dry materials so I would have to dry/fix cells to make it work (on the todo list!), but there are other AFMs that specialize in wet environments, like for alive cell cultures. There are even variants that can record the "adhesion" force, and it's used to help differentiate proteins on the surface of cells, since different proteins are more or less "sticky" than the surrounding cell membrane.
      There's a sorta-classic AFM experiment that looks at DNA which I might try some day. It's supposed to be pretty tough, but the results are neat when it works :)

    • @danielabbey7726
      @danielabbey7726 3 года назад +3

      @@BreakingTaps Cool! Thanks for the detailed explanation - very deep subject.

  • @michaelmazarakis6796
    @michaelmazarakis6796 3 года назад +9

    I find it odd how im a high-school drop out and i find myself watching videos like this.
    It also crazy how small that probe is.

    • @willfrank961
      @willfrank961 3 года назад +6

      Dropping out means the system has failed you. Not the other way around. It's not a testament to your character. Plenty of legendary thinkers have been fed up with or been failed by the system. If you're curious and rigorous then you're a scientist.

  • @LeoMakes
    @LeoMakes 3 года назад +1

    Super inspiring work! The "janky" prototype was actually my favorite part of the video! It made the concept very clear (you can't see a MEMS device working!)

  • @RiffZifnab
    @RiffZifnab 3 года назад +4

    "Glompy" I see you are also a person of Science. (:

  • @DEtchells
    @DEtchells 3 года назад +5

    WOW! That is *amazing* ! Excellent explainer, the “macro AFM” is great. I learned a lot; I’d always assumed that AFMs were measuring some sort of chemical-type interaction force. They were somewhat conflated in my mind with STMs (scanning tunneling microscopes). I’m **intensely** envious of (a) all your gear but (b) especially your new pro-level AFM. (Brilliant tech; when I saw your macro AFM, I immediately thought that flexures would be a great way to do the x/y movement, then saw that that’s exactly what the ICSPI unit uses, only built with MEMS technology. I wonder if I could 3D print a platform to carry the probe, using flexures? - And also wonder what the ultimate limits might be of your Macro AFM approach.
    ==> I know they only work on a “request a quote” basis, but *is there any way you could get ICSPI to let you tell us what the overall range of prices is* for the model you have? I assume there are a lot of different configurations, so likely a broad range of prices, but maybe they’d let you tell us the general range? I doubt I’d remotely be able to afford one, but would love to know I’d it’d ever be a possibility.
    (I used a mini-SEM in college for semiconductor research I was doing at the time; it was the most fun instrument I’ve ever used 😁)

    • @Gaetano.94
      @Gaetano.94 2 года назад +2

      Know a few people that worked on this tech from Waterloo and the ngauge costs around $10,000-$15,000

    • @DEtchells
      @DEtchells 2 года назад

      @@Gaetano.94 That actually seems remarkably inexpensive!👍

  • @RafaelAcurcio
    @RafaelAcurcio 3 года назад

    I'm in awe... I have never seen this kind of scanning. The quality of the scan from the home made version... Wow. The quality from the company one...wow! I can't even think of projects where I would use it. The area is really small, but the speed of the results... Impressive. Thank RUclips for the recommendation, subscribed.

  • @HolyGolyMoly
    @HolyGolyMoly 2 года назад

    What an absolutely staggering piece of equipment. Mind-Blown. Thank you very much for showcasing it for us, and for creating an overwhelming craving for this piece of equipment in myself and probably thousands of others. Hard to believe that such power can exist in such a compact, plug-and-play form.

  • @skrame01
    @skrame01 3 года назад +3

    Show the results, conclusions, recommrndations first, the best way to go.

  • @billpeiman8973
    @billpeiman8973 3 года назад +3

    I have investigated AFM before; the lowest price then was ~$25k.
    What is the price range for the nGauge ?

    • @jimquinn
      @jimquinn 3 года назад +5

      $15k with first 4 tips. Tips $200@ in 4pack ($800).

    • @blahblahblahblah2933
      @blahblahblahblah2933 3 года назад

      @@jimquinn That's not horrible if you have a need for it.

    • @billpeiman8973
      @billpeiman8973 3 года назад +1

      @@jimquinn What is the expected 'lifetime' of a tip?How is that measured?

    • @jimquinn
      @jimquinn 3 года назад

      $100@ ($400/4pk) std wedge tip, $200@ ($800/4pk) DLC sharp tip

    • @jimquinn
      @jimquinn 3 года назад

      @@billpeiman8973 hundreds of scans, see ICSPI's "tip" web page......

  • @chrisreichelt7202
    @chrisreichelt7202 3 года назад +1

    Super cool! Im imagining all the things I could scan.

  • @JayTee78NIN
    @JayTee78NIN 3 года назад

    I'm really grateful I found this channel today. It's always great to find a new interesting channel

  • @andie_pants
    @andie_pants 3 года назад +6

    _THAT_ is how you keep our ADHD asses from clicking away... hit us with the eye candy up front! :-D

  • @austinsloop9774
    @austinsloop9774 3 года назад

    great video! good flow, informational with great explanations, no fluff, and thanks for making the MacroAFM! So rare these days

  • @jersey-dude
    @jersey-dude 2 года назад

    Your enthusiasm makes me all that more excited about this device

  • @ivanvoid4910
    @ivanvoid4910 3 года назад +1

    best science and filming skills on utube combined for sure!

  • @JohnSmith-kf1fc
    @JohnSmith-kf1fc 2 года назад

    First video i see from you Breaking Taps and i gotta say this is one of the most underrated channel ive come across. You're good!

  • @XXxxGOOOFYxxXX
    @XXxxGOOOFYxxXX Год назад

    I really appreciate your enthusiasm. This video is super cool and helpful. Thank you.

  • @petergriffin1141
    @petergriffin1141 3 года назад

    absolutely loved it !! keep up the good work !!

  • @alphalunamare
    @alphalunamare 3 года назад

    Absolutely refreshingly fantastic!

  • @ryanbarakeris2853
    @ryanbarakeris2853 3 года назад +1

    He deffently valid for showing the results at the start

  • @sshado2
    @sshado2 3 года назад

    Thanks for teasing the photos up front. It makes me want to watch the rest!

  • @UNVIRUSLETALE
    @UNVIRUSLETALE 3 года назад

    Ok, this is REALLY cool, keep up with those amazing videos!

  • @epotis
    @epotis 3 года назад

    Fantastic video. Love the experimental soundtrack.

  • @DETHdressedInRED
    @DETHdressedInRED Год назад

    I like the style of your videos good sir! Start with the cool stuff and then capture my attention and then explain it, that way you don't lose me 3 minutes into the video. That deserves a sub.

  • @SuperiorDave
    @SuperiorDave 3 месяца назад

    Awesome pictures at the start. Thank you

  • @TriunfoGim
    @TriunfoGim Год назад

    Congrats to you and to ICSPI because both have done a good deal. I think the two of you should be pretty happy

  • @TheBauwssss
    @TheBauwssss 3 года назад +1

    Thank you very much for _not_ resorting to clickbait with your title for this video! Additionally, I want to give a second humongous thank you for showing those incredible AFM images right at the start of the video instead of forcing us to watch everything!
    Seriously, my thank you is very, very big and my appreciations are even bigger!! 😊 Not many RUclipsrs have this respect for the viewers, but you do. So you have my big congratulations, a very large thank you and so much appreciations! 😁👍

    • @BreakingTaps
      @BreakingTaps  3 года назад +1

      Thanks for the kind words! I was thinking about the video and how to structure it, and figured if I showed the images up front and folks _didn't_ want to see how those were generated, they probably wouldn't have watched the video long anyway. So might as well show them at the beginning so that everyone else could appreciate how cool it was at the beginning :) And I was just too excited to hold it in until the end haha :) Cheers!

    • @TheBauwssss
      @TheBauwssss 3 года назад

      @Stellvia Hoenheim "Clickbait is a text or a thumbnail link that is designed to attract attention and to entice users to follow that link and read, view, or listen to the linked piece of online content, *with a defining characteristic of being deceptive, typically sensationalized or misleading* "
      Source: en.m.wikipedia.org/wiki/Clickbait

  • @rexrodecolt
    @rexrodecolt 2 года назад

    Wow this is really cool of you to do, thank you! Very Interesting! Seriously!!

  • @oni2ink
    @oni2ink 3 года назад

    Your demonstration macro-AFM machine is pretty neat!
    And this nGauge AFM is really impressive, I really want to see some integrated circuits on it.

  • @squelchstuff
    @squelchstuff 3 года назад +1

    Fantastic exposition Zach. Congratulations on the deal you made with ICSP too, and thanks to them for making this possible. The axiom goes "Never read the comments" but your channel amongst a few others is an exception to the rule. I think I've spent more time enjoying the comments and thinking about what you've presented than the video actually lasted. I do hope to see more AFM microscopy, and perhaps a home brew setup too.

    • @BreakingTaps
      @BreakingTaps  3 года назад

      Agreed! I've learned a ton from folks that watch these videos, really happy the little community of folks that drop by to comment. So pleasant and knowledgeable! :)

  • @ryansauder7701
    @ryansauder7701 3 года назад +2

    Incredible tec, really neat to see. Thanks for sharing!
    I would love to see some of the laser induced graphene you made under the AFM!

  • @bur1t0
    @bur1t0 3 года назад

    Truly amazing. Not even at 30k subscribers and already I've seen people refer to this channel in the same sentence as Tech Ingredients, Thought Emporium, and Applied Science. I salute you!

  • @Michallote
    @Michallote 2 года назад

    I love the RUclips algorithm, it has lead me to some of the most awesome channels like yours, the summer of maths exposition was awesome. Thought emporium, and some other channel that speaks about Optics. I can't wait to graduate to start doing some experiments in my free time

  • @1.618_Murphy
    @1.618_Murphy 3 года назад

    You, my friend, is frickin underrated! And this is driving me nuts! 😭😭

  • @gaussmanv2
    @gaussmanv2 Год назад +2

    There's a bunch of really cool new types of AFMs that also have characterization like the nanoIR from bruker. The same ir peaks seen with an ftir also induce a greater volume change than other wavelengths. The AFM tip detects this change in the surface. You can get 10-20nm characterization resolution and it is very surface sensitive with a penetration of just a few nm. There's also a nano-raman and a nano-ftir.

  • @wmhilton-old
    @wmhilton-old 3 года назад

    This is ABSOLUTELY FREAKING COOL!!!

  • @HimanXK
    @HimanXK 3 месяца назад

    This is one of the best explanations of AFM I've seen

  • @1.4142
    @1.4142 2 года назад

    This topic is so really covered on youtube. subbed

  • @agargamer6759
    @agargamer6759 11 месяцев назад

    What a cool demo as well!

  • @SuperiorDave
    @SuperiorDave 3 месяца назад

    Awesome channel. Love the videos. I get so excited to watch the videos. I almost want to speed it up so i can watch them and absorb them faster

  • @DarkRedHorse
    @DarkRedHorse Год назад

    I don't comment on many videos, but man...this is seriously one of the coolest and most "I want to make this" videos I have seen in a long time. Thanks!

  • @lukasausen
    @lukasausen 2 года назад

    Love your channel im binge watching every video!

  • @sinephase
    @sinephase 3 года назад

    Awesome and congrats on the gig! :D

  • @TheClumsyFairy
    @TheClumsyFairy 3 года назад +1

    - So next week we will be building an MRI scanner...
    Like every time I see Breaking Taps I genuinely beam with excitement to see how the hell he's going to outdo the last video. And although he didn't say he would be building an MRI scanner, I bet most people thought "When did he say that", rather than "Don't be silly"..

  • @outofcontext728
    @outofcontext728 3 года назад

    Wow I got this in my recommended and I'm not disappointed this is amazing

  • @clfung2008
    @clfung2008 Год назад

    Cool thing ! This kind of microscopic equipment may become very popular and easy to get in the future ! Thanks for sharing and this is really interesting !

  • @Skwisgar2322
    @Skwisgar2322 3 года назад

    I was lucky enough to get to use one of these in my studies. Very cool stuff, we were able to see single atom thick features in graphite.

  • @KetilDuna
    @KetilDuna 3 года назад

    Contagiuos excitement. Thank you for sharing!

  • @smellsofbikes
    @smellsofbikes 3 года назад +1

    This is fantastic, it's a well-done video, and I loved the almost subliminal 'boop' when the probe contacted.

    • @BreakingTaps
      @BreakingTaps  3 года назад +1

      Thanks! ❤ I couldn't resist the boop haha 😂

  • @SonyFoLife
    @SonyFoLife 3 года назад

    Wow. Love this. Immediately subscribing! RUclips algorithm got me figured out by now

  • @jfcrow1
    @jfcrow1 3 года назад +1

    Amazing Technology. Interested in seeing the series.

  • @tobuslieven
    @tobuslieven 3 года назад +2

    They need to send one of these things to Applied Science, This Old Tony and Clickspring too. You guys are marketing gold dust. Well done for getting hold of one. Great video.

    • @tobuslieven
      @tobuslieven 3 года назад

      @Stellvia Hoenheim Don't shit your pants man.

  • @gmeast
    @gmeast 3 года назад

    I'm used to dealing with the likes of rocket motor turbo-pumps, but I must say, your presentation, here, is most satisfying in the realization that the world of macro vs. the world of micro share the same 'data' challenges ... I.O.W. ..."Parts are Parts". Thanks ... I've subscribed.

  • @edwardarruda7215
    @edwardarruda7215 2 года назад

    Used one in university physical chemistry lab experiments at Rutgers about 20 years ago. The one I used was on a heavy anti vibration table and it used a piezoelectric stage.

  • @agee1961
    @agee1961 3 года назад +2

    "An old 3d printer I had laying around" lol wow it is the future.

  • @Jandodev
    @Jandodev 3 года назад

    That scans so much faster than I was expecting!

  • @tituscassiusseverus6303
    @tituscassiusseverus6303 3 года назад

    I so need one of these, I have absolutely no idea why, but it's sooo damn cool!

  • @ColinTimmins
    @ColinTimmins 3 года назад

    Thank you to the company that worked with you so we can all see how this technology works. Great video. =]

  • @PatrickRatman
    @PatrickRatman Год назад

    honestly you having showed the results at the start of the video encouraged me to watch the rest of the video. most of the time i find it patronizing that i have to skip to the end to see the results.

  • @pontifier
    @pontifier 3 года назад

    This is amazing. I need one!

  • @leovalenzuela8368
    @leovalenzuela8368 3 года назад +2

    "...it seemed like a crime to wait till the end of the video to show those."
    Never have I ever subscribed to a new (to me) youtuber in my life.

  • @dcozombieloverr7115
    @dcozombieloverr7115 7 месяцев назад

    subbed, liked, etc. i respect you putting the results at the beginning of the video.

  • @jackking5567
    @jackking5567 3 года назад

    This is one of those things that I'd thought about that it could possibility be done and yet here we are not only has it been done but it's also a mass produced product with full support that does it very well and quickly. That in itself blows my mind never mind the small scale of scanning it can do.

  • @gokdude
    @gokdude 3 года назад

    First video I've seen of yours, and I subbed. 👍