Does all Disease Begin in the Gut? Monitoring the Barrier Function of an In Vitro Gut Mimic

Nearly 2500 years ago Hippocrates stated, “all disease begins in the gut”. We now understand that impairment of the barrier function of the gastrointestinal (GI) wall can have widespread repercussions on other organs in the body (e.g., brain and liver). To better explore the intricate relationships between the GI barrier and other organs, a predictive in vitro gut model is necessary. In this webinar, a Locsense scientist will evaluate the requirements of a representative GI mimic and provide a protocol to fabricate this in vitro model. Additionally, we will discuss impedance spectroscopy as a measurement tool to measure transepithelial electrical resistance (TEER) to analyze GI barrier function. Finally, we demonstrate the physiological relevance of the in vitro gut model by using chemical compounds to modulate the GI barrier function.