Yaaay! I'm glad you enjoyed the VOD. And don't worry about not being able to attend live. Hopefully we will do some more collabs in the near future! :D
That’s a filter similar to a darkfield patch stop filter. If you want to use phase contrast you will usually have a special condenser unit that has several interchangeable phase annuli filters/patch stops in. When you change objectives you can rotate a ring on the condenser to move the correct phase annuli patch for the current magnification.
to look for diatoms you should do it outside in nature instead of buying permanent slides on internet because fresh preparation are so much better than old permanent slides
There are many beautifully preserved antique diatom preparations and many of us that enjoy collecting them. Also, I did explain the process of how one can also collect them themselves which is the same method I plan to use to collect my own which I will also clean and mount wether they are new to science or not. I’m not sure what exactly your problem is but I feel this might not be the right content for you. Though from your comments I’m honestly not sure what possibly would be since you don’t like to see anything that isn’t new to science and it needs to be hand drawn? Seems rather specialist to me and probably not much of that content available on RUclips.
@@JessieTehEmoGurl show me me an antique permanet slide with diatoms which is better than a wetmount with my Zeiss axio and DIC or OI or even with REM. Show me a old permanet microtome cut of a stem which is better then mine on my Nicon Fluorescent. Show me an antique tardigrade which is better than mine with phase contrast.
@@JessieTehEmoGurl i am watching many videos on youtube for microscopy and i have also my own chanel ex ruclips.net/video/tg72vp3uCYE/видео.html and my own homepage. On my opinion some videos of microbehunter have faults and i tell my opinion. microbehunter is also selling care microscop stuff with amazon .....so this is a commercial chanel. He calls himself professor but has not even a DR. degree...But i critisize also other chanels which are so much better than his chanel like Microcosmos . From this i learn also things which i dont want to do.
there a may different ways to make microscopic documentation. The silliest way is to make permanent slides. Today we can make fotos and videos in a cheap fast and easy way. But the best way to make microscopic documentation is to draw by hand what you see.
@@JessieTehEmoGurl microscopy is not a hobby for me it is my profession. i am a scientist and investigator stedied on university mayring.in-ulm.de/autor/drrernat.jpg What formation on microscopy or biology have you criticising We dont use term microbe because it does not define the used microscope. Looking at bacteria with a REM or TEM you find cell organells and of course it is the perfect way of documentation to draw what you see beside the digital images. But today only few peopl can draw what they see this is real art
@@JessieTehEmoGurl are you a serious microscoper.......from what is see on your chanel you arr a gamer who likes shooting. i dont like that so i end the discussion with you here because i think i cannot learn anything from you
I’m a hobbyist, you clearly never watched the video… yes I’m also a tech nerd and gamer. I also do air arms target shooting. You’re the one who chose to be here and start the conversation not me. Happy holidays 🙋🏻♀️
those who search for esthetic pattern with a microscope are on the wrong way. Because you can find the same patterns without using a optic in macroscopic nature. These patterns only fractal structures or harmonic series following Fibonacci mathematical rules
Just because they can be found elsewhere doesn’t mean we shouldn’t look for them wherever we can and/or want to. If you don’t want to then you don’t have to. Myself and many others however enjoy it and will continue to do so.
@@JessieTehEmoGurl why should i look for esthetic patterns with a care instrument like microscop sitting on a chair in a stinking house for things i can find outside with my eyes
there is no reason to make permanent slides from known species because we have digital documentation rather cheap and easy. Also you can find very good and professional pictures on the internet for species you are looking for. Makes only sense if you find something new. But what i see from you is not new
There are many reasons one might want to make slides of samples that already exist. Firstly though, the ones i was showing in this stream are all antique slides i have collected from eBay auctions, not ones i have made myself. But i do plan to make my own too. Some good reasons to make slides with samples that already exist elsewhere is simply to have a higher quantity of them. If there was only ever 1 single preserved sample of a thing then only 1 person could study it at a time. Another reason is species variety. Many of the tardigrade you see form different people online look the same, but in fact are rarely the same species. You can find at least one new species of something in every persons yard. whether it is a bacteria, virus, micro-animal, diatom, algae, or something bigger like slugs and snails, worms or even insects. Just because it looks the same doesn't mean they are identical. You also need to practice on available subjects. You don't' want your first time ever mounting something to be a rare find that's never been seen before. It's highly likely that you are going to screw it up. It takes allot of practice to make good mounts. And another good reason to make your own is those who want to make their own personal collection. Think of someone who collects stamps and puts them in a book, or baseball cards, or how about Pokémon cards? These things are produced in the 10's of thousands per unit and yet everyone tries to get 1 of each and the end goal is everyone has the exact same collection. With slide craft every single one is different, even if the subject sample is the same. In terms of digital documentation, you will find that only about 1% of prepared slides are documented in any suitable way. On top of that, having only digital documentation to work from is impractical as you are bound by the scanners images. Maybe you would have liked to zoom in closer on a specific part that they didn't. You will also get a better image looking directly through the eyepieces at the subject than what you will get from a photomicrograph of it. Having the physical sample is just far more practical for actual research terms. And lets not forget the final reason to make your own permanent slides. And that is... fun! If it's not something you enjoy doing, don't do it. But there are plenty of people who do it as a fun little hobby and love doing it.
@@JessieTehEmoGurl most antique permanent slides are bad , especially those they sell on ebay. Some universities have old preparations. But these nowerdays are all documented digital because of decomposition. In my microscopic investigations i neither have the space nor the time to make permanent slides. Also it is much easier to find the preparations if they are in a digital database. For some species like the tardigardes i need raster electron images and order them. These images are so much better than with light microscopy and all are digital because the electron beam does destroy the surface to some extend, especially if you do EDX analysis. But i see that you are an hoby-microscoper and i do professional microscopic investigations and have my dr. rer. nat. degree from university. Finding new species or new knowledge is so much more exciting than collecting old boring stuff that everybody knows. I think you waist time and money doing this. I see that you are an beginner, i work over 50 years with microscopy and have over 20 light microscops some by DIY. You should look for something you dont know then make fotos and videos and search in books and internet if it is known insted of making or buying permanent slides
@@JessieTehEmoGurl this is a very silly way to end every discussion. Any scientific investigation is not made to make scientist happy also not me. You can look at nature with the eyes that god gave you to be perfect happy. But if you take a microscope to look at nature it makes you not happier because in macro you see the same as in micro. A microscope is made for scientific investigation not for fun or happiness
That feeling when your best buddy is in a stream 😊😄
I'm having a heart attack !!! I missed so great live ❤
Yaaay! I'm glad you enjoyed the VOD. And don't worry about not being able to attend live. Hopefully we will do some more collabs in the near future! :D
@JessieTehEmoGurl i hope so and looking forward to see it !
Non releited but what is phase contrast annuli filter?
That’s a filter similar to a darkfield patch stop filter. If you want to use phase contrast you will usually have a special condenser unit that has several interchangeable phase annuli filters/patch stops in. When you change objectives you can rotate a ring on the condenser to move the correct phase annuli patch for the current magnification.
@JessieTehEmoGurl thank you
@@Ebhin09 No problem, happy to help where i can! :)
49:26 Belege! La verda stelo.
no good idea to clean permanent slides with pure Ethanol or Acetone
to look for diatoms you should do it outside in nature instead of buying permanent slides on internet because fresh preparation are so much better than old permanent slides
There are many beautifully preserved antique diatom preparations and many of us that enjoy collecting them.
Also, I did explain the process of how one can also collect them themselves which is the same method I plan to use to collect my own which I will also clean and mount wether they are new to science or not.
I’m not sure what exactly your problem is but I feel this might not be the right content for you. Though from your comments I’m honestly not sure what possibly would be since you don’t like to see anything that isn’t new to science and it needs to be hand drawn? Seems rather specialist to me and probably not much of that content available on RUclips.
@@JessieTehEmoGurl show me me an antique permanet slide with diatoms which is better than a wetmount with my Zeiss axio and DIC or OI or even with REM. Show me a old permanet microtome cut of a stem which is better then mine on my Nicon Fluorescent. Show me an antique tardigrade which is better than mine with phase contrast.
@@JessieTehEmoGurl i am watching many videos on youtube for microscopy and i have also my own chanel ex ruclips.net/video/tg72vp3uCYE/видео.html
and my own homepage. On my opinion some videos of microbehunter have faults and i tell my opinion. microbehunter is also selling care microscop stuff with amazon .....so this is a commercial chanel. He calls himself professor but has not even a DR. degree...But i critisize also other chanels which are so much better than his chanel like Microcosmos . From this i learn also things which i dont want to do.
Again not sure what your problem is. Where did I say anything about slide being better than anything else?... Have a happy holiday season!
looks very nice.........but very less information on the biology of the species, which is most important on microscopy
there a may different ways to make microscopic documentation. The silliest way is to make permanent slides. Today we can make fotos and videos in a cheap fast and easy way. But the best way to make microscopic documentation is to draw by hand what you see.
You seriously need a new hobby if you think hand drawing is the best way to document microbes.
@@JessieTehEmoGurl microscopy is not a hobby for me it is my profession. i am a scientist and investigator stedied on university
mayring.in-ulm.de/autor/drrernat.jpg
What formation on microscopy or biology have you criticising
We dont use term microbe because it does not define the used microscope. Looking at bacteria with a REM or TEM you find cell organells and of course it is the perfect way of documentation to draw what you see beside the digital images. But today only few peopl can draw what they see this is real art
@@JessieTehEmoGurl are you a serious microscoper.......from what is see on your chanel you arr a gamer who likes shooting. i dont like that so i end the discussion with you here because i think i cannot learn anything from you
I’m a hobbyist, you clearly never watched the video… yes I’m also a tech nerd and gamer. I also do air arms target shooting.
You’re the one who chose to be here and start the conversation not me. Happy holidays 🙋🏻♀️
those who search for esthetic pattern with a microscope are on the wrong way. Because you can find the same patterns without using a optic in macroscopic nature. These patterns only fractal structures or harmonic series following Fibonacci mathematical rules
Just because they can be found elsewhere doesn’t mean we shouldn’t look for them wherever we can and/or want to. If you don’t want to then you don’t have to. Myself and many others however enjoy it and will continue to do so.
@@JessieTehEmoGurl why should i look for esthetic patterns with a care instrument like microscop sitting on a chair in a stinking house for things i can find outside with my eyes
Like I said, YOU, don’t have to… and it’s not my fault if your house stinks 🤷🏻♀️
@@JessieTehEmoGurl every house inside is stinky, here in Tenriffa i always sleep outside
there is no reason to make permanent slides from known species because we have digital documentation rather cheap and easy. Also you can find very good and professional pictures on the internet for species you are looking for. Makes only sense if you find something new. But what i see from you is not new
There are many reasons one might want to make slides of samples that already exist. Firstly though, the ones i was showing in this stream are all antique slides i have collected from eBay auctions, not ones i have made myself.
But i do plan to make my own too. Some good reasons to make slides with samples that already exist elsewhere is simply to have a higher quantity of them. If there was only ever 1 single preserved sample of a thing then only 1 person could study it at a time.
Another reason is species variety. Many of the tardigrade you see form different people online look the same, but in fact are rarely the same species. You can find at least one new species of something in every persons yard. whether it is a bacteria, virus, micro-animal, diatom, algae, or something bigger like slugs and snails, worms or even insects. Just because it looks the same doesn't mean they are identical.
You also need to practice on available subjects. You don't' want your first time ever mounting something to be a rare find that's never been seen before. It's highly likely that you are going to screw it up. It takes allot of practice to make good mounts.
And another good reason to make your own is those who want to make their own personal collection. Think of someone who collects stamps and puts them in a book, or baseball cards, or how about Pokémon cards? These things are produced in the 10's of thousands per unit and yet everyone tries to get 1 of each and the end goal is everyone has the exact same collection. With slide craft every single one is different, even if the subject sample is the same.
In terms of digital documentation, you will find that only about 1% of prepared slides are documented in any suitable way. On top of that, having only digital documentation to work from is impractical as you are bound by the scanners images. Maybe you would have liked to zoom in closer on a specific part that they didn't. You will also get a better image looking directly through the eyepieces at the subject than what you will get from a photomicrograph of it. Having the physical sample is just far more practical for actual research terms.
And lets not forget the final reason to make your own permanent slides. And that is... fun! If it's not something you enjoy doing, don't do it. But there are plenty of people who do it as a fun little hobby and love doing it.
@@JessieTehEmoGurl most antique permanent slides are bad , especially those they sell on ebay. Some universities have old preparations. But these nowerdays are all documented digital because of decomposition. In my microscopic investigations i neither have the space nor the time to make permanent slides. Also it is much easier to find the preparations if they are in a digital database. For some species like the tardigardes i need raster electron images and order them. These images are so much better than with light microscopy and all are digital because the electron beam does destroy the surface to some extend, especially if you do EDX analysis. But i see that you are an hoby-microscoper and i do professional microscopic investigations and have my dr. rer. nat. degree from university.
Finding new species or new knowledge is so much more exciting than collecting old boring stuff that everybody knows. I think you waist time and money doing this. I see that you are an beginner, i work over 50 years with microscopy and have over 20 light microscops some by DIY. You should look for something you dont know then make fotos and videos and search in books and internet if it is known insted of making or buying permanent slides
@@lotharmayring6063cool history, I’m glad it makes you happy. And I’ll continue doing what makes me happy too :)
@@JessieTehEmoGurl this is a very silly way to end every discussion. Any scientific investigation is not made to make scientist happy also not me. You can look at nature with the eyes that god gave you to be perfect happy. But if you take a microscope to look at nature it makes you not happier because in macro you see the same as in micro. A microscope is made for scientific investigation not for fun or happiness