Pump Wash and AKTA Avant

In chromatography air is the enemy. There is no way to ruin a perfectly good protein purification run then getting air on your column to start with. Using the pump wash command in Unicorn on the AKTA Avant and Pure you can get consistent runs without air.
For further instructions on how to prime your AKTA Avant's pumps see: cdn.cytivalife...
Reach at my Linkedin page: / chad-blamey-ph-d-649a336

is it really neccessary to do the pump wash for every run in ÄKTA pure / ÄKTA avant, 'cause I see that they always do a short pump wash when switch to other inlet possition?