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Does Opentrons distance themselves from the misuse of qPCR fragment screening as a medical diagnostic tool? Could they have done more publicly to save us from the abuses of the last 4 years?

1:34 robot and hardware were not aligned well... looks little bit strange

when is it going to be released?

Hi, Very nice videos ! Just one question: why your OT-2 has a tape on trash bin where normally there are the 'sensors' used for calibration ?

do you use monomer bio for your software?

Can this pipette 1 uL better than humans can for PCR? Can it transfer into a centrifuge?

🎉🎉

This truly seems like a hip product to make coffee in

Love that there is stamping now!

Amazing work! Really speaks to the soul of the modern scientist.

Oh man I wish I had this when I first set up an OT-2, this is an excellent resource! Just using the pictures in the software were a bit confusing for this step.

Nice Overview video, way to go Rami!

Not the best for Canadian or any non-US market. We are a sequencing core facility and would like to do a constructive critical review: Opentrons is a fully remote company with HQ in New York, NY. All support is remotely done via email ONLY. So just in case you have an issue in the middle of a run, the turnaround time is from 2-3 weeks (could go more to months) Opentrons does not offer onsite protocol development services outside of the 50 states of the U.S. So if you are outside of the US and if you request for support, no matter how dire the situation is no one will come physically nor on a zoom call to resolve the issue. Your automated handler will be in dust for weeks or months till you can set it up again. Not best for a busy lab. One issue leads to another and it never gets fixed. Opentrons claim to “get the OT setup running in 10min” is questionable, it has been almost 2 years and so far we haven’t done a single run, the tip crashes, the offset is off, the protocols provided to calibrate your OT2 are given wrongly without knowing what consumables you have. Moreover, even when troubleshooting, despite giving all information, the redundancy in their queries is high that it is questionable if it is in their best interests to support Opentrons user, mostly superficially looking into issues. Opentrons do not check what consumables or protocols were paid for, you may have to repeatedly clarify what you have bought. Your territory manager, application specialist etc. are not Cc’ed in most emails, so you kind of don’t know who you are dealing with and this adds on the “complexity” that no one is assigned to your case. You will end repeating your issues over and over again to different people, due to their department being re-organized several times. To summarize, if you are outside of US, I would strongly suggest not to go for this, but go for a company that supports you locally, who can actually fix issues and who are transparent in emails. Automation should make life easier. If you really need things to move fast with a quick turnaround time for support, then Opentrons is not your best bet. You can reach out to the centre on more information and see it also, before you make a decision to buy Opentrons.

Hello, I am experiencing an issue when I select calibrate now it loads for a few seconds then nothing happens? Could you assist please?.

🥳

Great

🔥🤝

Hanzhen harmonic drive gear , strain wave reducer, robot joint , over 30 years experience

Hanzhen harmonic drive gear , strain wave reducer, robot joint , over 30 years experience

Hi Nick, can I use the magnetic module for for tube cleanup

Can this robot transfer liquid from 15-25 ml conical tubes in a rack to 96 well microplanes, 1-200 ul volumes?

they really helped with Covid detection research

Single-Channel Electronic Pipette is 900$ !! omg it is very expensive for a stepper motor and a syringe

p

If you didn't sound like you're in a basement people might take you seriously.

Still waiting for liquid level tracking ;) This would make the OT2 a fully competitive liquid handling robot. I know, you can program workarounds, which I already did, but it would open up so many more users.

Very cool!

Is it possible to extend the space from OT-2 and make the work bench bigger? Great product. Unfortunately my department doesn't have one.

With temperature and thermocycler, what's the end price?

Also custom modules for "edge cases" unlike some companies, the "Open Source" nature allows you to make custom "add ins" work with the system without having to reverse engineer / hack things.

I'm exited to see more content! (Also i think "The Thought Emporium" recently aquired one, so there will be even more content on it) There definetly aren't enough "Open Hardware Groups" out there, and you seem to have a great product that can save some "research assistant" (or just people tired of doing piles of pipetting) time. (And for a reasonable cost compared to some pharmecutical / high end lab device + open source so custom toolheads etc can be made) I'm kind of rambling, but keep up the good work!

The device seems bigger than i remember! I though in pictures/skimming websites that it was the size of a small 3d printer, but it's the size of a medium or so one. More "Real Estate" for modules and all that, just suprised me seeing a "human for scale" etc.

No

Maybe they boast a little bit, but Opentrons are much cheaper. The accuracy is acceptable for many labs.

How can it do miniprep? Does it have the ability to centrifuge samples and pipet only the supernatant?

Why does this have 3.2k views but only 12 likes and no comments? Is RUclips alright?

The pipetting performance displayed in this video absolutely does not meet or even exceeds the performance of more expensive units from Hamilton/Tecan/PerkinElmer/Eppendorf/... You show plenty of liquid sticking to the tip outside and residual liquid when discarding the tips. I have read your white paper on your claimed performance, this isn't it.

The "Download run app" url (opentrons.com/software) gives a 404 error: Page not found. I think the page has moved to opentrons.com/ot-app.

Very interesting and inspiring