![Food Microbiology Lab](/img/default-banner.jpg)
- Видео 22
- Просмотров 50 857
Food Microbiology Lab
Добавлен 16 фев 2020
Видео
Calculating Results from Counted Agar Plates
Просмотров 1073 года назад
Calculating Results from Counted Agar Plates
Thermoduric and Psychrotrophic Bacteria Lab Video
Просмотров 5643 года назад
Thermoduric and Psychrotrophic Bacteria Lab Video
Thermoduric and Psychrotrophic Bacteria Background
Просмотров 9933 года назад
Thermoduric and Psychrotrophic Bacteria Background
E. coli and Coliforms Background Relationships
Просмотров 2,3 тыс.3 года назад
E. coli and Coliforms Background Relationships
Can you upload salmonella testing according to usfda and BAM
Buraya da imzamizi atalim 😺
Thank you, very useful video
Y cant we use wireloop instead of these wooden sticks
You probably could use a wire loop, but the wooden sticks come with the agglutination kit, so we used them.
Reference website if
Reference please
What is the best antibiotic for e coli and coliform bacteria infection thanks
Hi Vincent. The use of antibiotics really depend on many factors - mainly host, pathogen, and antimicrobial factors. Host factors are the factors that are attributed to the person or animal that is being infected, such as the species being treated, other conditions or medications they have that could interfere, disease severity, etc. When considering pathogen factors, we need to think about resistance and virulence factors (which can often be attributed to chronicity and severity of infection) the pathogen may have. We can do this by looking at local trends in resistance. For example, in some areas, we've seen widespread resistance to beta-lactams in E. coli. If an E. coli infection was in this area, we might think about using an alternative class due to the resistance that is known. Lastly, antimicrobial factors need to be considered. We need to consider the components of a therapeutic regimen. In humans and animals, we may need to consider various routes of administration or how frequently it can be given. In animals used for food, we need to be conscientious of meat or milk withholding times. As you can see, there are generally a lot of things that go into what makes a drug best, and there isn't a "best" drug for ALL situations; there are just better choices for specific situations over others. The use of antimicrobials should be carefully considered for each individual situation (which is also a big component of antimicrobial stewardship). β-lactams, fluoroquinolones, aminoglycosides and trimethoprim-sulfamethoxazole have all been used to treat E. coli infections in human hospital settings, but it's not a one-size fits all.
I think in your second example, the combination should be 322 instead of 321
Hi! You may want to go back through and re-watch the video. 3-2-1 is correct, and I explained the reasoning behind it.
Thank you for explaining how we choose dilutions to work with
Why blue colour changes to milky white after incubation
I think you may be getting the RV tubes a bit confused. The white tube is tetrathionate broth, while the blue tube is Rappaport-Vassiliadis. Post-inoculation, the blue tube just becomes cloudy as seen in the picture.
amazing video thanks 😍😍😍😍
Is there any strain that eats c. Diff or c. Perfrigens?
please upload bacillus cerus video
Hi - these were done for a class that I was teaching. We didn't cover B. cereus in lab, so there will not be a video uploaded for this.
Good
There are machines that “pound” kinda like shakes the media and sample to simulate stomach/digesting to help increase/speed for specie enrichment and diffusion.
Merci beaucoup
What is bpw ? Sorry.
buffer peptone water
Thanks for explaining in a very good way.
what do you mean when you said stomach the sample?
A machine called a stomacher (also called paddle blenders) has paddles move back and forth that basically help to mix the sample to ensure that the bacteria are mixed throughout the sample. It essentially expels bacteria or other microbes from a solid (i.e., a food product in this case) into the liquid also added to the sample bag.
What if none of them are all positive and 1st and 2nd lowest dulutions are all negative. For example-0,0,2,1,0
You likely need to improve your assay and/or ensure you're performing dilutions correctly as each sample is a dilution of the previous one and your results should be reflective of that. If you're getting higher numbers of positive samples on the 3rd and 4th dilutions than you are with the 1st and 2nd, there's likely an issue somewhere else.
How much do you know about this confirmation latex kit? Does the size of the agglutination matter? I see here you got veeeery tiny agglutination, sometimes the agglutination looks veeery big (have you ever experienced that?) Does the size matter?
I don't work with them a ton, but in my experience, the amount of agglutination you get is usually related to how much of the organism is included. In this case, we didn't have a lot of sample, which could be why the degree of agglutination wasn't as pronounced.