You likely need to improve your assay and/or ensure you're performing dilutions correctly as each sample is a dilution of the previous one and your results should be reflective of that. If you're getting higher numbers of positive samples on the 3rd and 4th dilutions than you are with the 1st and 2nd, there's likely an issue somewhere else.
Thank you for explaining how we choose dilutions to work with
when picking the middle dilution rate, does it matter which kind of table we use?
Thank you, very useful video
I think in your second example, the combination should be 322 instead of 321
Hi! You may want to go back through and re-watch the video. 3-2-1 is correct, and I explained the reasoning behind it.
What if none of them are all positive and 1st and 2nd lowest dulutions are all negative. For example-0,0,2,1,0
You likely need to improve your assay and/or ensure you're performing dilutions correctly as each sample is a dilution of the previous one and your results should be reflective of that. If you're getting higher numbers of positive samples on the 3rd and 4th dilutions than you are with the 1st and 2nd, there's likely an issue somewhere else.