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Microscopy Australia
Австралия
Добавлен 14 апр 2020
Australia's largest network of microscopes and expertise - open to all Australian researchers. Formerly AMMRF. An NCRIS facility.
All work published on this channel is is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License and is the IP of the institution that the talk is produced from.
These videos provide guidance only. Local work health and safety protocols should always take precedence when you undertake any lab procedures in your own facility. Some of the videos show workflows for specific instruments and their content should not be taken as specific instructions for other types of instruments.
All work published on this channel is is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License and is the IP of the institution that the talk is produced from.
These videos provide guidance only. Local work health and safety protocols should always take precedence when you undertake any lab procedures in your own facility. Some of the videos show workflows for specific instruments and their content should not be taken as specific instructions for other types of instruments.
VIA Webinar: Volume Imaging with a Synchrotron – Now and in 10 years time
In this webinar Dr. Chris Hall, Senior Scientist, Imaging and Medical Therapy, ANSTO, will introduce the existing facility and what it can do for volume imaging with photons.
The nascent plans for a new machine will be presented, with an invitation to forward ideas from researchers for desired machine parameters and instruments.
This webinar is presented by Volume Imaging Australia, a special interest group of the Australian Microscopy and Microanalysis Society (AMMS), and Microscopy Australia.
The nascent plans for a new machine will be presented, with an invitation to forward ideas from researchers for desired machine parameters and instruments.
This webinar is presented by Volume Imaging Australia, a special interest group of the Australian Microscopy and Microanalysis Society (AMMS), and Microscopy Australia.
Просмотров: 91
Видео
README Files for Microscopy Data
Просмотров 455 месяцев назад
Users of microscopy facilities can generate 10s to 100,000s of digital image files in a single scientific experiment. In this video learn: - the importance of documentation for your research data - how to create a simple README file for microscopy images Links The University of Adelaide Research Data and Primary Materials Policy can be viewed at www.adelaide.edu.au/policies/4043/ I suggest chec...
Directory Structures for Microscopy Data
Просмотров 365 месяцев назад
Users of microscopy facilities can generate 10s to 100,000s of digital image files in a single scientific experiment. In this video: - understand the value of a well-organised directory structure - learn strategies to organise your own image datasets Links The University of Adelaide Research Data and Primary Materials Policy can be viewed at www.adelaide.edu.au/policies/4043/ I suggest checking...
File Naming Convention in 7 STEPS: Worked Example for Biological Data
Просмотров 635 месяцев назад
Users of microscopy facilities can generate 10s to 100,000s of digital image files in a single scientific experiment. In this video follow a 7-step process to systematically create a file naming convention for microscopy data in the biological sciences Links The University of Adelaide Research Data and Primary Materials Policy can be viewed at www.adelaide.edu.au/policies/4043/ I suggest checki...
File Naming Conventions for Microscopy Images
Просмотров 655 месяцев назад
Users of microscopy facilities can generate 10s to 100,000s of digital image files in a single scientific experiment. In this video learn: - the benefits of file naming conventions - how physical specimens transform into digital filenames Links The University of Adelaide Research Data and Primary Materials Policy can be viewed at www.adelaide.edu.au/microscopy/facilities-services/data-access#ho...
Why You Should Manage Your Microscopy Image Data
Просмотров 675 месяцев назад
Users of microscopy facilities can generate 10s to 100,000s of digital image files in a single scientific experiment. In this video, learn the benefits of effective (and burdens of ineffective) data management. Links The University of Adelaide Research Data and Primary Materials Policy can be viewed at www.adelaide.edu.au/policies/4043/ I suggest checking with your home institution for any poli...
Insitu TEM Masterclass Lectures
Просмотров 543Год назад
This Microscopy Australia Masterclass presented lectures on recent progress in insitu TEM imaging and spectroscopy of materials properties and dynamics under different stimuli. Jump to the talk you are interested in using the links below. 00:00:26 “Why do we perform in-situ experiments?” Richard Tilley (University of New South Wales) 00:20:25 Q/A session with Chao Zhang (Queensland University o...
BYO Workshop: Introduction to ORS Dragonfly with Dr Denis Korneev
Просмотров 608Год назад
Dragonfly software is an integrated platform for volume image processing. It includes all standard tools for processing of 3D stacks as well as an deep learning tools for advanced segmentation. The software can be used to process FIB-SEM, confocal, X-Ray, and other datasets. This online workshop was recorded in May 2023 as part of the Volume Imaging Australia Build Your Own Workshop Series. The...
Assessing data quality in minutes - the near real time data capture revolution in microscopy
Просмотров 102Год назад
In this webinar Josh Silver, Research Data Officer (University of Wollongong), will explore the impact of the near-real-time data capture revolution in microscopy from an IT perspective. Within a few years, technological progress and optimisation of workflows have enabled instrument scientists and researchers to assess the quality of datasets in minutes rather than days. Much of this near-real-...
Data Management at the Centre for Microscopy, Characterisation and Analysis by Dr Emily Barker
Просмотров 105Год назад
In this webinar Dr Emily Barker will reviews the challenges and opportunities for data management at the Centre for Microscopy, Characterisation and Analysis at the University of Western Australia. The Centre for Microscopy, Characterisation and Analysis (CMCA) has existed since the 1960s under several names. While it started as the Electron Microscopy Centre, it now hosts instruments for many ...
Increasing University wide Research Data Management maturity by Yvette Wharton and Laura Armstrong
Просмотров 52Год назад
'Increasing University-wide Research Data Management maturity - a journey' presented by Yvette Wharton and Laura Armstrong (University of Auckland, NZ). ABSTRACT Research Data Management (RDM) is increasingly recognised as a critical knowledge gap for researchers as international and domestic funders, publishers, and ethics committees introduce more stringent requirements regarding Data Managem...
GBI EoE VII Impact and UN Sustainible Development Goals NIF and MA
Просмотров 24Год назад
Recorded presentation by Microscopy Australia and the National Imaging Facility to the GBI Exchange of Experience VII meeting 2022. It demonstrates alignment of these facilities' outcomes to the UN's Sustainable Development Goals.
Video Rate Atomic Force Microscopy - Direct Visualization of Interfacial Nanostructure and Dynamics
Просмотров 255Год назад
In this technique discovery webinar Dr Hua Li, Scanning Probe Microscopy Platform leader at CMCA UWA, will take us through how advances in video-rate AFM can be used to understand nanostructure and dynamics of liquid-solid interfaces. Abstract: An atomic force microscope (AFM) uses a sharp tip to scan across a solid surface. The interaction between the AFM tip and the surface is measured and co...
BYO Workshop: Introduction to IMOD presented by Dr Matthew Johnson
Просмотров 2,8 тыс.Год назад
Volume Imaging Australia presents the next installment in their Build Your Own Workshop Series: Introduction to IMOD. IMOD software is a set of image processing programs for image analysis, modelling and displaying 3D data from instruments generating image stacks. The workshop covers: IMOD Installation Basic use of IMOD and 3dmod Tomogram alignment Tomogram reconstructions Intro to Segmentation...
What does Microscopy Australia do?
Просмотров 1032 года назад
Happy Science Week! Here at Microscopy Australia we give researchers access to Australia's largest network of high-end microscopes, and the expert scientists who operate them. This video is part of a series of videos developed for National Science Week 2022 explaining how the various NCRIS facilities enable great research in Australia.
Pitschi Particle Image depoT using Storage Caching Infrastructure by Dr Rubbiya Ali and Dr Hoang Ngu
Просмотров 1162 года назад
Pitschi Particle Image depoT using Storage Caching Infrastructure by Dr Rubbiya Ali and Dr Hoang Ngu
BYO Workshop: Visualising 3D data for Publication by Dr Ajay Limaye and Dr Jeremy Shaw
Просмотров 6742 года назад
BYO Workshop: Visualising 3D data for Publication by Dr Ajay Limaye and Dr Jeremy Shaw
AARNet: Data movement as a service and GLOBUS for Australian research by Chris Myers and Ryan Fraser
Просмотров 1022 года назад
AARNet: Data movement as a service and GLOBUS for Australian research by Chris Myers and Ryan Fraser
An introduction to Australian Access Federation (AAF) services including ORCID by Melroy Almeida
Просмотров 562 года назад
An introduction to Australian Access Federation (AAF) services including ORCID by Melroy Almeida
Principles of research data management for FAIRer microscopy presented by Dr David Poger
Просмотров 1012 года назад
Principles of research data management for FAIRer microscopy presented by Dr David Poger
MyScope Simulator Launch: TEM, XRD & Light/Confocal
Просмотров 6492 года назад
MyScope Simulator Launch: TEM, XRD & Light/Confocal
Basic Operation of the Hitachi SU3500 SEM
Просмотров 3,6 тыс.2 года назад
Basic Operation of the Hitachi SU3500 SEM
Tutorial: Re-orientation of 3D volumes in 3DSlicer by Dr Jeremy Shaw
Просмотров 9 тыс.2 года назад
Tutorial: Re-orientation of 3D volumes in 3DSlicer by Dr Jeremy Shaw
Tutorial: Re-orientation of 3D volumes in Avizo by Dr Jeremy Shaw
Просмотров 3,7 тыс.2 года назад
Tutorial: Re-orientation of 3D volumes in Avizo by Dr Jeremy Shaw
The Research Data Champion Program at Curtin University
Просмотров 712 года назад
The Research Data Champion Program at Curtin University
TEM as a Research Infrastructure in Europe by Prof. Rafal Dunin-Borkowski
Просмотров 3182 года назад
TEM as a Research Infrastructure in Europe by Prof. Rafal Dunin-Borkowski
In Situ TEM Symposium hosted by the Electron Microscope Unit, MWAC UNSW
Просмотров 6772 года назад
In Situ TEM Symposium hosted by the Electron Microscope Unit, MWAC UNSW
Persistent Identifiers for Research by Siobhann McCafferty from ARDC
Просмотров 1812 года назад
Persistent Identifiers for Research by Siobhann McCafferty from ARDC
The Australian Characterisation Commons at Scale Project Update July 2021 by Dr David Poger
Просмотров 1123 года назад
The Australian Characterisation Commons at Scale Project Update July 2021 by Dr David Poger
Thanks very much!
Won't the labeling marker ink cause an issue in the SEM Vacuum chamber?
Much appreciated!
Thank you
Fascinating and well presented. Thank you for sharing this knowledge for free
Really well explained! Thanks!
How to cut a 100mm silicon disk into small pieces (>10mm)?
thanks
During importing a file you are taken (@ 5.21 mins) as .txm files. How to create this .txm files for images?
Hi Parameswaren, thanks for your question. The .txm file is a proprietary file format created by the Zeiss microCT system. Avizo has the ability to import this file format. You cannot create this file unless you have the instrument. However, Avizo will allow you to import and export data in a range of other open source formats (such as TIFF or DICOM). You can just select this from the drop down list in the save function. Hope that helps.
it should be double or 70%
Easy and Interesting
hi, i want to know, what should I do if my sample cannot stick to double side adhesive ? I tried other tape, the sample still not attached…
In general, we tend not to use adhesives to mount nanomaterials for SEM examination. As you can see in the video, we usually create a well-dispersed suspension of particles and deposit a drop of that suspension onto a clean, featureless (preferably conductive) substrate such as a silicon wafer. Once the suspension liquid fully evaporates, we then image the remaining nanoparticulate residue in the SEM.
Thank you so much. Really nice presentation
Thank you!
Hi there, I want to analyze the permeability and tortuosity of a porous structure. Could you please provide me with any help or tutorial? Thanks
The information here is a little out dated. The ice issue is only exists in LN2 detectors which can be barely seen on SEMs nowadays. Modern EDS has great improvement in terms of efficiency and low-kV X-ray detection. The data in the example is from 1998. The spectrum processing at that period is not a good as modern EDS software, especially on peak overlapping issue and background subtraction. It's good to see the comparison with WDS. Lots of people over relied on EDS thus ignored WDS. Thank you for the presentation.
I worked in a EDS company. This is by far the best presentation I've seen about EDS analysis. Most importantly, it's based on practical experience that you need to know or be aware of in the analysis. Also, it's not quite easy to put all the sessions into one hour talk. Good job and thank you for the presentation.
Wow....I learnt alot
Glad it was helpful!
what on earth is this resin
How much amount needed to sent for a sem analysis test of nanoparticle
Thank you so much
Amazing presentation! I got a lot of ideas from your presentation. It helps with how to deal with SEM images.Thank you very much.
thank you very much
This information was very helpful to me.. Thanks for giving such amazing explanation 🙏👍
Glad to hear that :)
Many thanks.
Why is liquid carbon dioxide used??
Thank you sir
Great lecture. Thank you for sharing.
Thanks for the good video. I got a broken microscope EVO MA-10. Based on your videos, I fixed it and learned how to work on it.
Great to hear!
wow
Is it necessary to dry the prepared nano particles prepared by wet chemical method?
Yes, samples need to be vacuum stable and should be fully dry before attempting to image them in a SEM. Even in an ESEM instrument, which can handle a certain degree of moisture, the SEM is only capable of imaging the surface of the sample, so will not be able to ‘see’ nanoparticles suspended inside a liquid.
This is by far the best demonstration video I have ever watched.
To find the appropriate spot size, aperture and all the other parameters seems not so easy. How do you find them? Are there some rules?
Very informative.👍
I am very thankful for your video. This is very informative and really helpful Thanks
Am using this to make glass knife ,bt now I dnt knw what happens both sides r cmg sharp.thr is no blunt edge. How do I rectify ths
thnx
Amazing, thank you so much
Glad you liked it!
It seems that all the transformation was still done in the anatomical coordinate system (RAS). I can't seem to find a way to do the transformation and resampling in the IJK space.
Thank you! This is very helpful!
You're very welcome!
This is one of the nicest lectures I have ever seen on this topic...Thank you.
I’m a Veterinary from Brasil and what to thank you a lot for this incredible lesson.
Glad you enjoyed it!
great information thanks, but i can not rotate my image with this steps ☹
Hi Navnath, can you explain where you are having trouble?
Hello! I have one problem with Avizo. I work with snake skulls and make 3D models of the whole skulls. Once, I didnt finish the whole skull, just made some layers of one bone and then closed Avizo (Save project as and then Auto Save). On the other day, I opened Avizo and in Volume-1. labels (where you have four screens - XY, XZ, YZ and 3D view) I had only that selected bone (colored bone) but on the black background where I didnt have the rest of the skull = other bones that should be visible in greyish colour on the background. I have tried to close Avizo and then open it again but the situation was the same, so I cannot selected other bones to obtain 3D model of the whole bone. Thank you for any advices! :)
Hi Simona, I'll check in with our Micro-CT team and see if they have a solution :)
Hi Simona, sounds like you only saved the binary label field (the coloured one) using the auto save project method you selected. Not sure what version of Avizo you are using, but when you save the project you should select the "pack and go" option in the "save as type" dropdown list before clicking save. The "greyish" coloured data is probably your original data. Just drag and drop (or other opening method) this back into Avizo and save it again. Personally, I avoid saving projects unless I have to these days. I prefer saving only the very necessary files separately. Otherwise, you can end up with a lot of unwanted data saved.
Thank you for this very nice and informative video...
Glad it was helpful :)
helped me a lot! Thank you!
Glad it helped!
Nice !
a carrot as an embedding medium- mind blown- this is clever
Thanks for the video! It was informative.
hello excellent lesson and PDF is there any way i can try this out on the turtle head DICOM data? would love to play with that scan