- Видео 6
- Просмотров 23 494
Amira Mahdi
Ирландия
Добавлен 21 дек 2021
BE553 Topic V Tutorial qPCR relative analysis using 2–∆∆Ct
Tutorial 1 in Topic V for course BE553 as part of the Precision Medicine MSc. degree at Dublin City University. In this video we conduct relative analysis of qPCR data using the 2-∆∆Ct method
Просмотров: 9 812
Видео
BE553 Topic III Tutorial 2 ELISA experimental setup and analysis
Просмотров 6372 года назад
Tutorial 2 in Topic III for course BE553 as part of the Precision Medicine MSc. degree at Dublin City University. In this video we go through the experimental set up and analysis of an ELISA experiment.
BE553 -Topic 2 Tutorial 2: Analysing viability assays and IC50s
Просмотров 12 тыс.2 года назад
In this video we will 1. Analyse the results of an MTT based cell viability assay, looking at the effect of doxorubicin on cell lines 2. Graph the results in the form of a dose response curve 3. Calculcate the IC50 for doxorubicin in this cell line 4. Graph the results as a bar chart and statistically analyse the difference between drug concentrations using an ANOVA.
BE553 Topic II Tutorial 1 Setting up a drug viablity assay
Просмотров 7412 года назад
Tutorial 1 in Topic II for course BE553 as part of the Precision Medicine MSc. degree at Dublin City University. In this video I explain how to set up an experiment to assess the viability of cells following treatment with a chemotherapeutic drug. at 6:08 it should be micromolar not microliter!
BE553 Topic I Tutorial 2 Cell Number Calculations
Просмотров 3552 года назад
Tutorial 2 in Topic I for course BE553 as part of the Precision Medicine MSc. degree at Dublin City University. In this video I go through how to calculate your cell density following counting and the volume of cell suspension needed to set up an experiment.
BE553 Topic I Tutorial 1 Cell Counting
Просмотров 3502 года назад
Tutorial 1 in Topic I for course BE553 as part of the Precision Medicine MSc. degree at Dublin City University. In this video I go through how to use a heamocytometer to count cells for cell culture experiment and how to do the associated calculations.
Hi Amira! you are awesome! WHy dont you put more videos and help us? Thanks a lot
Currently writing my thesis and your video answered all the questions I had regarding the data and analysis. Thank you very much!!!🙌🏽🙌🏽
you just saved my life!!! excelent video, it helped me a lot!
how can do with spss?
hello how can do the statistical analysis as i dont have graphpad prism??
I have benefited a lot from this video, thanks!
Thank you so much mam. Actually it was very much informative and detailed well with the test performed. I have a request like please upload more videos regarding graphpad prism and analysis. Thank you. This video is worthy
how to enter data 3 table? it is not explained. All other things are well explained and are very helpful. Thank you.
thank you so much for this. helped a lot
Thank you so much for the videos. It helped a lot!
thnku v much Amira. Please make videos on every assay. plus Flow cytometry and TEM SEM flourscent microscopy... etc etc. u r an excellent teacher.
this is a great explanation, and so helpful, thank you!
So helpful!!!
Gracias
Perfect! Thank you!
hi, what about vehicle sample? do i need to inlucde in the dose-response curve ?
Thank you soo much, Dr.! So helpful.
Thank you so much for this!!!
Very informative can you tell me how to make graph for more than one tissue sample
hi why did you use parametric instead of nonparametric test ?
i DO have the three sets of data and i wonder how to do it
Why is your "total volume needed" 10 mL? If you are using only six wells wouldn't the total volume be 0.7 mL? (0.7 if you consider 7 wells to have enough).
That's great. Thanks Amira.
Thanks a lot! It's everything I wanted!!
Thank you so much for this!
email for contact?
Thank you very much for this tutorial video, Dr. Mahdi.
What do you mean repeat for all other experiments for the cell viability percentage (time 11:07)
same question!
your videos are great, why you stopped them really you explain in a very simple way with all details
Thank you so much. It is really appreciative to watch this video.
very very nice instruction, Thanks so much.
Hi Amira...hope you keeping well...Amira plz I need your help in analysis...if you can show me....am badly stucked
Nice video... Can i get this xcl template?