Perfect. I learn More and More from your description. I have never seen these kind of TEM description. The operators in our lab have never allowed us to touch such a microscope. By seeing this video my imagination boost . Thanks a lot.
It's really nice to hear some background information, thanks. I was only introduced to the software of the new devices, which can be kinda overwhelming in the beginning regarding all the adjustments if you have never dealt with TEM before.
it would be great if u can enlighten me how to prepare sample of gold nanoparticle for its characterization using TEM technique. you have shown liquid sample needs sampler loader with the clamp and it requires that carbon grid for sample loading. i want to know how the liquid sample taken like in my case i have gold nanoparticle as my sample..should i just handover my nanoparticle solution to get TEM done and they would take a drop or two from the whole solution..or does it required any purification step or some other step before taking down on the grid..please guide
9 лет назад
It should be just straight forward exactly as you suggested. If it is of high concentration or lots of organic materials like surface acting agents, dilution is very much recomended.
Sir which is the best Microscope for Experiments. Like I want to start my own Research in my Home . Starting with the Bacteria and then Viruses. Please recommend me the Microscope which is suitable for this work. Thank you so much Sir.
Does it matter which side of the copper grid you put your solution based sample on; the dull or the glossy side?
4 года назад
One side will expose the copper mesh making the solution to be trapped in each square of the mech by capillary forces and, this tends to accumulate to much liquid. Also depending on type of grid you could have a two layer grid, carbon supported by plastic, in that case you will have two surfaces of different hybrofobicity to play with.
@ sir can you explain how staining works in TEM? Does the stained area appear white or dark and why?
3 года назад
@@chiasmata8625 Staining is usually done with some heavy element material, like uranium, or more explicit a salt of uranium. Because it is massive it will give you a darkening contrast effect. Note that uranium-salts are poisonous and should be treated there after.
3 года назад
@@chiasmata8625 That is correct, if using bright field imaging.
@ thank you sir.. Before, I actually thought that the electron stained areas will appear lighter as electrons won't pass through.. I thought that since more electrons passed through the non stained areas, it would be darker but now I understand clearly.. Once again, thank you very much sir!
Perfect. I learn More and More from your description. I have never seen these kind of TEM description. The operators in our lab have never allowed us to touch such a microscope. By seeing this video my imagination boost . Thanks a lot.
mehrdad r thanks!
It's really nice to hear some background information, thanks. I was only introduced to the software of the new devices, which can be kinda overwhelming in the beginning regarding all the adjustments if you have never dealt with TEM before.
you are doing a great job..its totaly worth a watch to easily comprehend all the information related to TEM. thanks for sharing
thank you so much for this video astonishingly detailed. great job Magnus.
I just want you to know, I appreciate your videos, thank you!
That's a great and useful video for beginners..
Thanks so much for this video
This demo is really very useful. Thank you!
Thanks, Magnus. Straight forward explanation.
Thank you for the video. Helped me a lot.
Hello Dear, Thank you for sharing such good information. And keep it up. Thanks again...
NICELY EXPLANATION
Check out my Lego version of a Transmission Electron Microscope ideas.lego.com/projects/102281
Christopher Olson -Cool lego!
It is sooo cool!!!!!!!!!!!!!!!
good video
so great explanation .. thank u
it would be great if u can enlighten me how to prepare sample of gold nanoparticle for its characterization using TEM technique. you have shown liquid sample needs sampler loader with the clamp and it requires that carbon grid for sample loading. i want to know how the liquid sample taken like in my case i have gold nanoparticle as my sample..should i just handover my nanoparticle solution to get TEM done and they would take a drop or two from the whole solution..or does it required any purification step or some other step before taking down on the grid..please guide
It should be just straight forward exactly as you suggested. If it is of high concentration or lots of organic materials like surface acting agents, dilution is very much recomended.
Thank you ..
soo when are we gonna get the temmie flakes?
It important
Sir how can I contact with you
A. Banerjee (India)
What''s the issue?
Sir which is the best Microscope for Experiments. Like I want to start my own Research in my Home . Starting with the Bacteria and then Viruses. Please recommend me the Microscope which is suitable for this work. Thank you so much Sir.
quite old machine even in 2014.
That's correct.
Does it matter which side of the copper grid you put your solution based sample on; the dull or the glossy side?
One side will expose the copper mesh making the solution to be trapped in each square of the mech by capillary forces and, this tends to accumulate to much liquid. Also depending on type of grid you could have a two layer grid, carbon supported by plastic, in that case you will have two surfaces of different hybrofobicity to play with.
@ sir can you explain how staining works in TEM? Does the stained area appear white or dark and why?
@@chiasmata8625 Staining is usually done with some heavy element material, like uranium, or more explicit a salt of uranium. Because it is massive it will give you a darkening contrast effect. Note that uranium-salts are poisonous and should be treated there after.
@@chiasmata8625 That is correct, if using bright field imaging.
@ thank you sir.. Before, I actually thought that the electron stained areas will appear lighter as electrons won't pass through.. I thought that since more electrons passed through the non stained areas, it would be darker but now I understand clearly.. Once again, thank you very much sir!
Holly ... You've spent so much time explaining your microscope as if all your viewers would have a similar one at hand.
Please see tobbaco mosaic virus and coronavirus in microscope like
thank you so much for this video astonishingly detailed. great job Magnus.