Microbiology 102 = Sterility Testing | Pyrogen Testing By Rabbit Method | LAL Test for Pyrogen

Download the "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - play.google.co...
Limulus Amoebocyte Lysate Test
The limulus amoebocyte lysate (LAL) test is a simple method for the detection of viable and non-viable Gram-negative bacteria. Certain cell-wall lipopolysaccharides (i.e. endotoxins) of this bacterial group lead to gelation of blood cell (amoebocytes) lysates of the Limulus polyphemus crab. Using a dilution row and determining the limit at which no more gel formation occurs, a semi-quantitative estimation of the Gram-negative content is possible. Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebocytes) from the Atlantic horseshoe crab Limulus polyphemus. LAL reacts with bacterial endotoxin lipopolysaccharide (LPS), which is a membrane component of gram-negative bacteria. This reaction is the basis of the LAL test, which is widely used for the detection and quantification of bacterial endotoxins.
Inject sterile pyrogen free saline solution intravenously at a dose of 10 ml per kg of body weight. Record the temperature of animals 90 minutes prior to the injection and continue for 3 hours at an interval of half an hour after injection. Exclude any animal showing temperature variation of 0.6 or more for main test.
Get in touch with the solution by just clicking the following links-
Facebook Group- / solutionpharamcy
Mobile Application - play.google.co...
New Channel (Pharmacy Dictionary) / @pharmacydictionary
Instagram- / solutionpharmacy
E-Mail for official and other work - [e-mail]
LinkedIn- / pushpendrakpatel
#solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPATonlinetest #GPATclass #GPATvideos #Microbiologyclass#Microbiology#

[@vandanaprajapati2611]

Thanku so much sir...✨Best teacher in pharmacology...and also now in microbiology..😇😇😊😊

[@solutionpharmacy]

Thank you so much for your kind support and feedback

[@msdhoni071]

Thanku sir ❤ really helpful 😊

[@solutionpharmacy]

Most welcome 😊

[@Nature-qb1sh]

Thank you sir❤

[@solutionpharmacy]

Most welcome

[@masrurakhatun4765]

Thanks is a little words 😊!! For u sirr!!! But ...thanks a lots❤

[@39himanshuzilka62]

Really helpful in industry pharmacy topic too... ❤️ Thank you sir you are unique.. 🌟

[@solutionpharmacy]

Thank you

[@Shivom_06]

Thank you ❤🎉

[@panchalsir]

Your teaching is great sir...

[@dheerajkumarvishwakarma8357]

Thanks sir

[@Indiandipuk]

Dhanyabad

[@careerpharma3005]

Nice explanation sir

[@KrishnaMedicare]

Good evening sir

[@syedghulammuhammad265]

Plz explain what is pyrogen biologically and chemically

[@pratikgaji]

Thank you sir