How to watch CELL RESPIRATION under the microscope

Today I will show you how you can make CO2 bubbles visible under the microscope. By adding sugar to yeast, the yeast will start to respire and produce carbon dioxide. These bubbles can be seen under the microscope.
0:00: 🔬 Observing carbon dioxide bubbles produced by yeast cells under a microscope.
1:47: 🔬 Observing the fusion of yeast bubbles under a microscope.
3:44: 🔬 Observing yeast cells under a microscope and preparing them for observation.
#carbondioxide #yeast #microscope
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[@spencebarton2947]

Thanks for the interesting video. You say the colors were a result of the specific optics that you have. Would that be polarization with retardation plates? Doesn't look like phase contrast or DIC. Inquiring minds want to know.

[@MarieChardome]

hi. this is supercool. and i will definitely play with oblique . omg, MH, i am still playing with urine crystals and cannot get enough of the beauty it creates! trying out condenser, light intensity, filters i understand not much about yet (on a OLY CH-b). not enough lives to do all this hahaaa. thank you so much for the happiness you diffuse around the globe.

[@İsmailPakyol]

Really nice video, thanks for sharing.. 👍👏👏

[@Dom6879]

Beautiful colors

[@StoreSuccess]

💐💐

[@Jegbonto]

What I can't underszand is how the co2 ends up in the large bubble if yeast is producing it on the outer edge of those black clumps. I dont see any small bubbles forming that wold merge into the large. Is it happening on a scale we can't see?

[@Manuel-cx6ob]

Physics student here, that's a great question!
In summary: the big bubbles mainly absorb co2 directly from the water, since there is co2 dissolved in water. I'll try explaining it below.
When CO2 is emitted by microorganisms, it does not immediately form bubbles. A certain amount of CO2 can be dissolved in water. You can imagine this dissolved co2 as if it had a "pressure", i.e. it wants to get out of the water, forming bubbles. At the same time, water molecules really like to stay close together, so they create their own pressure working against the formation of bubbles. The size of the bubbles plays an important role here. Small bubbles are harder to form, because they have greater surface area per unit volume, meaning the water can produce more force per unit volume. But, if there is some solid object that already displaced some water (we call it "nucleation site"), it allows for a bigger initial bubble (lower surface area to volume ratio), making it possible for the bubble to grow with lower co2 concentration that it would need to create a new bubble from scratch. So the big bubbles seem to grow into even bigger bubbles, seemingly out of nowhere.

[@Jegbonto]

It clicked as soon as I saw your first sentence. Makes perfect sense now that gases can dissolve into water. Thanks

[@edithaviland8461]

Amazing