I have no reason to be watching this, I don't know why it was recommended. But I watched the whole thing bc it was super interesting. I had no idea how these things were done
Yes, this is called the brush technique. You can find an in-depth description of the brush technique on the website IHC World: A Method for Preparation of Frozen Sections (www.ihcworld.com/_protocols/histology/frozen_section_technique_1.htm). When using this technique, the brush should be held like a pen and the hand can be stabilized by resting one finger on the stage. The pressure applied on the section when sliding it across the stage should be minimal as to avoid damage or the section adhering onto the stage. The brush should move at the same speed as the section sliding on the stage.
This is a good training video, especially for those beginners like me. Thanks.
I have no reason to be watching this, I don't know why it was recommended. But I watched the whole thing bc it was super interesting. I had no idea how these things were done
Whether keeping tissue in paraformaldehyde won't lead to cross-linking
Is it good to section the tissue without anti-roll glass? as what you did in the video?
Yes, this is called the brush technique. You can find an in-depth description of the brush technique on the website IHC World: A Method for Preparation of Frozen Sections (www.ihcworld.com/_protocols/histology/frozen_section_technique_1.htm).
When using this technique, the brush should be held like a pen and the hand can be stabilized by resting one finger on the stage. The pressure applied on the section when sliding it across the stage should be minimal as to avoid damage or the section adhering onto the stage. The brush should move at the same speed as the section sliding on the stage.