This video is exactly what I was looking for. I had trouble to understand super resolution microscopy, and now I think I understood the core concept of it. Thank you so much!!
Cool! She is a very highly regarded scientist, and helped create a nobel prize winning technology. Many people think she should have been awarded as well, but it is limited to 3 people and hard to choose which are most deserving.
Thanks for breaking this down so simply. It is amazing just how detailed the images are and humbling to realize that each of the cells imaged are so vastly complex and compact. Exciting to imagine how much further this can be improved. Thanks again I really felt like I learned a lot.
I am only 6 minutes in and already I approve of your presentation. I am a layperson who studies the sciences purely for self enlightenment. Unfortunately I was unable to pursue my passion for a science career in my youth due to financial issues. Nicely done and very informative.
(On Monday of April 17, 2023). On the Matter of PhD Xiaowei Zhuang from Howard Hughes Medical Institute and Harvard University (Boston, MA) on the Topic of Super-Resolution Fluorescence Microscopy and the Biophysics (Sub-Diffraction Limit) of Light Microscopy: 1) Monoclonal Antibodies or Chemotherapeutic Drugs are quite Limited in Specificity And In Part has to do with the Inability to Test and Verify the Pharmaceutical Claims which usually go completely uncontested as long as FDA Clinical Procedure has been even lethargically Exercised at the Risk and almost always fulfilled to Ineffective and Very costly Therapeutics. Even Methotrexate, An Antifolate Drug and Mechanism therein could use specification and Reaction times of Binding because the Finesse of the Antagonism is what would be more of Benefit than the mere--frankly ineffective--Gross and Crass narration/Effect of Cytotoxicity. Anyway, the Imaging of Fluorescence (STORM, PALM et al) has its limits but certainly a vast amount of Proteomics can be and has been understood or investigated. My personal favorite is the Metabolism Visualization of the Voracious and Dysregulated Rate of Reproduction via Hyperactive NTP Biosynthesis, I mean its Entropy at its best, and it has been Elucidated by this Modality of Microscopy Fairly. 2) The Embryology and Dysgenesis or Aplasia Therein is a rather nice manner to Understand and Highly likely to be of vast benefit In the Effort to Prevent such Lamentable and Often Detrimental Metabolic Recessive Diseases (Incidence and Prevalence Management is not a Legitimate CDC Function) where a Defective Protein is Post Facto understood rather than before its Formation and Proteogenic Defect Manifests Pathology. Perhaps also the Methylome of DNA (Protein and Nucleic Acid Visualization) could be in Vivo Tagged and be of Diagnostic/Therapeutic value, especially in favor of Prophylaxis of pathology rather than to blindly, scientific professionals' most flagrant misgiving to Mankind, see the effect, or even more, most disgustingly, to Palliate. 3) In Short, I am glad this Technology is Available and Disseminatable because another distressing Parameter in Pure Science and Research And Investigations Currently is its Political and National Security Factor. There is never a Winner in this Scenario even the Morbidly and Moribund Industrial Complex of Death and Destruction. This is currently my upmost concern because Progress is dependent obviously in the Pursuit of Profit and War while a time Before it was a Matter of Emancipation and Cultivation. We can all forget this bodacious Revery if the known tyrannical Procedures continue and even Prosper. PhD Zhuang, es geht gut aber es muss sollten gehabt schnelller sein. Heil!
It will be good for cryonicists and I have the same disease than Howard Hughes almost. But I hope this tool will widely be used by scientists in the future to see if we can save our own lives. like in anti aging. all around the world. in germany, in france, in china, in canada, in usa etc..
for cryonicists we will need more than that, more than 10 NM resolution, and not only cells outsides the bodies, but inside the bodies like in a MRI, while the patient is living or not, like frozen or where there are movement. This tool was implemented in 2006 I think or so, and perfected in 2018 or 2016. Let's put another 100 years and we will be able to see in a resolution of less than 1 nanometers, everywhere inside living bodies or objects with movements inside. we will need scanners of 0.1 nm of resolutions for living patients.
We weren't filming in HD at the time we recorded the talk. However, check out www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/super-resolution-microscopy.html and the videos here www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course.html as most of them were in HD.
Isn't that all Green Screen that you are doing? Couldn't the background be incorporated at a higher resolution and the video rerendered at 720p or 1080p? I realize that the foreground lecturer will not be in HD, which may look a bit weird, but the important images in data will be more clear.
one of the worst speaker. slow words, inadequate aggressive intonations, lack of facial expressions. almost impossible to perceive such presentation, if you are not a masochist, of course.
This video is exactly what I was looking for. I had trouble to understand super resolution microscopy, and now I think I understood the core concept of it. Thank you so much!!
I was so surprised to come across this video that found out you were one of my students in Suzhou High School in 1985.
chiNAZI DETECTED....
Cool! She is a very highly regarded scientist, and helped create a nobel prize winning technology. Many people think she should have been awarded as well, but it is limited to 3 people and hard to choose which are most deserving.
Thanks for breaking this down so simply. It is amazing just how detailed the images are and humbling to realize that each of the cells imaged are so vastly complex and compact. Exciting to imagine how much further this can be improved. Thanks again I really felt like I learned a lot.
Fantastic presentation. Really impressed by the clarity and usefulness of this information. Thank you very much.
Thank you; one of the best science channels on the Tubes
Many thanks for this great presentation, very clearly explained.
But now we just need to win the lottery to get a storm instrument to play with.
I am only 6 minutes in and already I approve of your presentation. I am a layperson who studies the sciences purely for self enlightenment. Unfortunately I was unable to pursue my passion for a science career in my youth due to financial issues. Nicely done and very informative.
LARP.
A very good, and coherent explanation with plenty to take away for even a layman such as myself! Thank you! rc
Great promising Technology,great presentation.Thank you.
Really beautiful presentation!
Great presentation and great explanation.
Thank you so much for amazing presentation. It was really intuitive ... And you have good humor :) ;) ...
Thanks for uploading this!
thanks really beautiful Pf.Zhuanga lot.Your video is really help!
Awesome talk. Thank you
still mind-blowing, such elegant method
Thank you - very helpful!
thank you for the great class. :D
(On Monday of April 17, 2023). On the Matter of PhD Xiaowei Zhuang from Howard Hughes Medical Institute and Harvard University (Boston, MA) on the Topic of Super-Resolution Fluorescence Microscopy and the Biophysics (Sub-Diffraction Limit) of Light Microscopy: 1) Monoclonal Antibodies or Chemotherapeutic Drugs are quite Limited in Specificity And In Part has to do with the Inability to Test and Verify the Pharmaceutical Claims which usually go completely uncontested as long as FDA Clinical Procedure has been even lethargically Exercised at the Risk and almost always fulfilled to Ineffective and Very costly Therapeutics. Even Methotrexate, An Antifolate Drug and Mechanism therein could use specification and Reaction times of Binding because the Finesse of the Antagonism is what would be more of Benefit than the mere--frankly ineffective--Gross and Crass narration/Effect of Cytotoxicity. Anyway, the Imaging of Fluorescence (STORM, PALM et al) has its limits but certainly a vast amount of Proteomics can be and has been understood or investigated. My personal favorite is the Metabolism Visualization of the Voracious and Dysregulated Rate of Reproduction via Hyperactive NTP Biosynthesis, I mean its Entropy at its best, and it has been Elucidated by this Modality of Microscopy Fairly. 2) The Embryology and Dysgenesis or Aplasia Therein is a rather nice manner to Understand and Highly likely to be of vast benefit In the Effort to Prevent such Lamentable and Often Detrimental Metabolic Recessive Diseases (Incidence and Prevalence Management is not a Legitimate CDC Function) where a Defective Protein is Post Facto understood rather than before its Formation and Proteogenic Defect Manifests Pathology. Perhaps also the Methylome of DNA (Protein and Nucleic Acid Visualization) could be in Vivo Tagged and be of Diagnostic/Therapeutic value, especially in favor of Prophylaxis of pathology rather than to blindly, scientific professionals' most flagrant misgiving to Mankind, see the effect, or even more, most disgustingly, to Palliate. 3) In Short, I am glad this Technology is Available and Disseminatable because another distressing Parameter in Pure Science and Research And Investigations Currently is its Political and National Security Factor. There is never a Winner in this Scenario even the Morbidly and Moribund Industrial Complex of Death and Destruction. This is currently my upmost concern because Progress is dependent obviously in the Pursuit of Profit and War while a time Before it was a Matter of Emancipation and Cultivation. We can all forget this bodacious Revery if the known tyrannical Procedures continue and even Prosper. PhD Zhuang, es geht gut aber es muss sollten gehabt schnelller sein. Heil!
THANK YOU!
fantastic!
Very impressive. Only one question, why comic sans font? There are hundreds of good fonts out there.
You are genius. Hope to have a chance to work in your group.
Awesome!
Very impressive presentation. And such a beautiful lady with such a magical laugh.
小威老师，我的偶像！
really amazing
It will be good for cryonicists and I have the same disease than Howard Hughes almost. But I hope this tool will widely be used by scientists in the future to see if we can save our own lives. like in anti aging. all around the world. in germany, in france, in china, in canada, in usa etc..
for cryonicists we will need more than that, more than 10 NM resolution, and not only cells outsides the bodies, but inside the bodies like in a MRI, while the patient is living or not, like frozen or where there are movement. This tool was implemented in 2006 I think or so, and perfected in 2018 or 2016. Let's put another 100 years and we will be able to see in a resolution of less than 1 nanometers, everywhere inside living bodies or objects with movements inside.
we will need scanners of 0.1 nm of resolutions for living patients.
awesome
Any chance that a lecture on Super-Resolution Microscopy could be posted as a video in High Definition? 480p max... seriously?
We weren't filming in HD at the time we recorded the talk. However, check out www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/super-resolution-microscopy.html and the videos here www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course.html as most of them were in HD.
Isn't that all Green Screen that you are doing? Couldn't the background be incorporated at a higher resolution and the video rerendered at 720p or 1080p? I realize that the foreground lecturer will not be in HD, which may look a bit weird, but the important images in data will be more clear.
Use a looking glass!
super
2:57
I think it's scanning probe not spinning probe.
Genius
The scale of sizes going the wrong way down drives me crazy.
still unsharp imges and the resolution cannot be better then the wavelength of the fluorescent light
14:52
tip: watch in 2x speed
good tip..**
1.25 is better
I prefer 0.5x
Hahaha, I did the same thing and then saw your comment as I was about to post the same thing.
chinese chosaky, sage chini
Holy Shit, this is 13 year old tech? Is there a movie being made about images from the molecular universe?
Comic Sans?! WAT?!!1
why not? lol
I immediately closed the video.
480 p here sucks.
at 1.25x speed she doesn't sound drunk
Robbed of Nobel.
Exactly! Robbled of Nobel! Extremely unfair! The Nobel price is totally nonsense and ridiculous!
one of the worst speaker. slow words, inadequate aggressive intonations, lack of facial expressions. almost impossible to perceive such presentation, if you are not a masochist, of course.
awesome