Thanks for the explanantion. I still don't get 1 thing. If there are 2 or more copies of target gene with different lengths, how do we know if the other ones are also our target gene, or do we need to do DNA sequencing after that?
Why do we use electrophoresis to separate the different length DNA? Isn't the restriction enzyme cutting arbitrary, meaning the lengths don't actually mean anything? Would it matter if the band was shown in the larger DNA segments or the smaller ones(the ones that travelled more)
A question, what if the restriction enzyme cleaves the gene of interest in the DNA? Wouldn't the probe show two bands even though the gene is present once?
I don't know if you need an answer anymore, but here we are: For this technique, the choice of the restriction enzyme is crucial because it shouldn't cut within the gene~ otherwise wouldn't be able to quantify the number of loci of your sample
Purpose:Southern Blotting: Detects specific DNA sequences.RFLP: Detects variations in DNA sequences among individuals or populations.Procedure:Southern Blotting: Involves gel electrophoresis, membrane transfer, and hybridization with labeled probes.RFLP: Requires DNA digestion with restriction enzymes and gel electrophoresis.Applications:Southern Blotting: Used for gene mapping, rearrangement detection, copy number variation analysis, and DNA methylation analysis.RFLP: Used in genetic linkage mapping, paternity testing, forensic analysis, and studying disease-associated genetic variations.Main Output:Southern Blotting: Shows presence or absence of specific DNA sequences.RFLP: Displays variations in DNA fragment lengths.
If the Dna échantillons if from the same person or nah" like for exemple in crime scene they take the blood found on ground... and analyse it by this southern blot technique😊
Thank you for this - awesome explanation and very concise
Please make video about western blotting technic.thanks a million. YOUR video is more easier than the others.
Legend. Thanks for this
Well done - thank you very much!
This was really useful. Thanks.
honestly fairs, this video was good
Why we need to separate double stranded DNA into single stranded DNA?
So that the fluorescent probe can bind to the complementary sequence.
very brief and comprehensive video on southern blotting
Thank you very much sir ..
Very nice explanation
Thank you for this nice video. this was helpful :)
Absolutely magnificent vedio sir
Helix wrap logically would extrapolate the subjects spin trajectory.... Deciphering the wrap is a challenge...
Peace Love always ♥️🙏
Thanks a lot
Thanks for the explanantion.
I still don't get 1 thing. If there are 2 or more copies of target gene with different lengths, how do we know if the other ones are also our target gene, or do we need to do DNA sequencing after that?
all the band we see are complementary to the probe --> all the band we see are target gene
Thank you!!!!!!!
Fabulous ❤
Devru macha neenu!🖤🍺
Expland well ly thnkx
Why do we use electrophoresis to separate the different length DNA? Isn't the restriction enzyme cutting arbitrary, meaning the lengths don't actually mean anything? Would it matter if the band was shown in the larger DNA segments or the smaller ones(the ones that travelled more)
In electrophoresis length of DNA fragments play great role because we set all optimal conditions according to the length or size of DNA fragments❤ .
Nice❤❤
Nice video
A question, what if the restriction enzyme cleaves the gene of interest in the DNA? Wouldn't the probe show two bands even though the gene is present once?
I don't know if you need an answer anymore, but here we are:
For this technique, the choice of the restriction enzyme is crucial because it shouldn't cut within the gene~ otherwise wouldn't be able to quantify the number of loci of your sample
@@fyre1116 Thanks
What's the difference between southern blotting and restriction fragment length polymorphism
Purpose:Southern Blotting: Detects specific DNA sequences.RFLP: Detects variations in DNA sequences among individuals or populations.Procedure:Southern Blotting: Involves gel electrophoresis, membrane transfer, and hybridization with labeled probes.RFLP: Requires DNA digestion with restriction enzymes and gel electrophoresis.Applications:Southern Blotting: Used for gene mapping, rearrangement detection, copy number variation analysis, and DNA methylation analysis.RFLP: Used in genetic linkage mapping, paternity testing, forensic analysis, and studying disease-associated genetic variations.Main Output:Southern Blotting: Shows presence or absence of specific DNA sequences.RFLP: Displays variations in DNA fragment lengths.
I love you
This technique is used in ?
DNA Fingerprinting
If the Dna échantillons if from the same person or nah" like for exemple in crime scene they take the blood found on ground... and analyse it by this southern blot technique😊
Thank you so much
@@nounou53 But how can they get that this blood belongs to that specific person? Do they have everyone's DNA?
@@someone-yj2im police might have some criminals dna pattern who once escaped
nice video. but why are you speaking in 0.5x speed
Then increase the speed😕
Bruh, hes just trynna be clear wdym. Would you rather he spoke fast but not be understandable
Western blotting required