Southern Blot

[@AdityaSingh-hh2jr]

Thank you for this - awesome explanation and very concise

[@24valenciapoh40]

Thank you for this amazing video!

[@mdreadwan8619]

Please make video about western blotting technic.thanks a million. YOUR video is more easier than the others.

[@LeaveMeAlune]

Wonderful as usual, you saved me many times

[@ivegotbadtaste9181]

Legend. Thanks for this

[@missbhardwaj1694]

Why we need to separate double stranded DNA into single stranded DNA?

[@gammagongetya8967]

So that the fluorescent probe can bind to the complementary sequence.

[@jammywow1805]

This was really useful. Thanks.

[@robzplayz6749]

honestly fairs, this video was good

[@21-sadmanali.24]

Absolutely magnificent vedio sir

[@fatemashaikh7465]

Very nice explanation

[@thesixthcourier]

Well done - thank you very much!

[@ahmedkhalil8264]

Thank you for this nice video. this was helpful :)

[@biologyexpert586]

very brief and comprehensive video on southern blotting

[@aashishchinchure6605]

Devru macha neenu!🖤🍺

[@boho0114]

Thank you very much sir ..

[@prayoga4419]

Thanks for the explanantion.
I still don't get 1 thing. If there are 2 or more copies of target gene with different lengths, how do we know if the other ones are also our target gene, or do we need to do DNA sequencing after that?

[@ginocaprino]

all the band we see are complementary to the probe --> all the band we see are target gene

[@bobu5213]

A question, what if the restriction enzyme cleaves the gene of interest in the DNA? Wouldn't the probe show two bands even though the gene is present once?

[@fyre1116]

I don't know if you need an answer anymore, but here we are:
For this technique, the choice of the restriction enzyme is crucial because it shouldn't cut within the gene~ otherwise wouldn't be able to quantify the number of loci of your sample

[@bobu5213]

@@fyre1116 Thanks

[@orkunakman1541]

Why do we use electrophoresis to separate the different length DNA? Isn't the restriction enzyme cutting arbitrary, meaning the lengths don't actually mean anything? Would it matter if the band was shown in the larger DNA segments or the smaller ones(the ones that travelled more)

[@usamaosama7157]

In electrophoresis length of DNA fragments play great role because we set all optimal conditions according to the length or size of DNA fragments❤ .

[@adlesal24]

Fabulous ❤

[@ricasaluria8821]

This technique is used in ?

[@kavyaarts4927]

DNA Fingerprinting

[@nounou53]

If the Dna échantillons if from the same person or nah" like for exemple in crime scene they take the blood found on ground... and analyse it by this southern blot technique😊

[@ricasaluria8821]

Thank you so much

[@someone-yj2im]

@@nounou53 But how can they get that this blood belongs to that specific person? Do they have everyone's DNA?

[@nammarcbfan2529]

@@someone-yj2im police might have some criminals dna pattern who once escaped

[@nissiabaidoo4693]

What's the difference between southern blotting and restriction fragment length polymorphism

[@KeyaPanchal-hf6gb]

Purpose:Southern Blotting: Detects specific DNA sequences.RFLP: Detects variations in DNA sequences among individuals or populations.Procedure:Southern Blotting: Involves gel electrophoresis, membrane transfer, and hybridization with labeled probes.RFLP: Requires DNA digestion with restriction enzymes and gel electrophoresis.Applications:Southern Blotting: Used for gene mapping, rearrangement detection, copy number variation analysis, and DNA methylation analysis.RFLP: Used in genetic linkage mapping, paternity testing, forensic analysis, and studying disease-associated genetic variations.Main Output:Southern Blotting: Shows presence or absence of specific DNA sequences.RFLP: Displays variations in DNA fragment lengths.

[@ammarahmehak9986]

Nice video

[@MsVelandra]

Thanks a lot

[@kashafsaleem6173]

Nice❤❤

[@richardshane456]

Helix wrap logically would extrapolate the subjects spin trajectory.... Deciphering the wrap is a challenge...
Peace Love always ♥️🙏

[@sanafakih3541]

Thank you!!!!!!!

[@ekhushimanzoor6186]

Expland well ly thnkx

[@avonleastudy6535]

nice video. but why are you speaking in 0.5x speed

[@rosette_renah]

Then increase the speed😕

[@hridyanshusinghroy2570]

Bruh, hes just trynna be clear wdym. Would you rather he spoke fast but not be understandable

[@amrit.oo7]

😂

[@Godxspeedbgmi]

Western blotting required

[@plaisanterie9551]

I love you

[@justso9281]

your videos are amazing! you helped me A LOT every single time!🥲