Lecture 06, concept 20: Potential of mean force - free energy along a reaction coordinate

[@danielcheong4804]

Is x the reaction coordinate here? I'm thinking that v(x) is the PFM, so I'm taking it as the PMF V is a function of x, which is the reaction coordinate.

[@SuperVITHURSHAN]

sir, how to calculate the binding free energy from PMF?