Sir, i am a btech student and i saw TEM in our lab where lab instructor explained us each and every part of TEM So, at 19:00-20:00, i think you mistakenly tell wrong it should be opposite of what you tell The higher mass thikness region will show dark colour because intensity of electron beam transmitted from this region of specimen will be low Similarly, the zero mass thickness region will show white colour because whole intensity of electron beam will be transmitted from this region of specimen BTW, lecture was nice 😊
What an amazing talent is this. Simplified the topic so that it can reach anyone who listens to this, Thanks a lot, sir. This is really a divine service
Sir, first of all watch your all lectures which were nice I may be Wrong but at time 20:00 the white colour in TEM is represent absence of atoms and black colour represent presence of atoms of specific shapes as we did TEM in our Lab.
No I think you are wrong. Black reprenets nothing and gray or gray white colour represents the electron beam from the sample are more passed.. Overall the image is of gray white scale.. I might be wrong😊
Ye jo aapne baat boli dil se padha raha hun i am also a teacher main deakh shakta tha ki ye. Aat bilkul sach hai ki aap bahut dil se padha rahe the ki ye chij real life me kaise kaaam karti hai.
bahut acche se aap concept clear kr dete ho bhaiay , ek hi baar mein pura samaj aa jata h. thanks yo u bhaiya aapke wajah se mere mid mein acche marks aaye
When I was watching your vedios I remember those days when I used to study from physicswallah in class 12 ..your way of teaching is like him but I can't compare you with him he is the legend he is the bestesttt but I must say that I've seen many vedios of graduation topics till now but yours is the best literally I've never enjoyed studying graduation topic this much.. you are so confident and your way of teaching is awesome now I can suggest you as best teacher for graduation and post graduation physics 👍
Vese m comment nhi krti hu but aako dekh k alakh pandey sir ki yaad aa gyi Aaj Mera exam h and this video is very useful for me thank you so much apne bht clear explanation diya ❤❤❤keep it up
I can't understand 1) how electrons pass solid lenses? 2)if secondary electron are replaced primery electron so how can generate dark image? 3) if we don't know the shape of any cell so how can made solid metal frame for it ?
@bhagwatprasadbaghel7185 1) Rather than having a glass lens focusing the light (as in the case of light microscopes), the TEM employs an electromagnetic lens which focuses the electrons into a very fine beam, which means those are not solid lenses they are circular electromagnetic lenses. 2)When the beams strike the phosphor image screen light is generated, which causes the image to be visible to the operator. The area through which very few electrons are transmitted look darker (they are thicker or denser). The lighter areas of the image represent those areas of the sample through which more electrons were transmitted (they are thinner or less dense). In the video at first he explained wrongly and later he explained correctly. 3) Specimens must be very thin so that electrons are able to pass through the tissue. This may be done by cutting very thin slices of a specimen’s tissue using an ultramicrotome. The tissue must first be put in a chemical solution to preserve the cell structure. The tissue must also be completely dehydrated (all water removed). Once preserved and dehydrated, tissue samples are placed in hard, clean plastic. The plastic supports the tissue while it is being thinly cut with the ultramicrotome(you can google the image of this device). After sections are cut and mounted on grids, (tiny circular disks with openings,) a solution of lead is used to stain the tissue. The lead provides contrast to the tissue by staining certain cell parts. When placed in the electron microscope, the electrons are scattered by the lead. They do not penetrate the tissue or hit the fluorescent screen, leaving those areas dark. We don't find the shape of the cell to prepare a metal frame for it, In the video solid metal frame means the metal absorbed by some parts of cell.
Sir aap subscribe and like ke liye nhi bolte hai kyuki aap ko apne mehnat pe bharosa hai, thank you so much sir for giving this lovely lecture
Sir, i am a btech student and i saw TEM in our lab where lab instructor explained us each and every part of TEM
So, at 19:00-20:00, i think you mistakenly tell wrong it should be opposite of what you tell
The higher mass thikness region will show dark colour because intensity of electron beam transmitted from this region of specimen will be low
Similarly, the zero mass thickness region will show white colour because whole intensity of electron beam will be transmitted from this region of specimen
BTW, lecture was nice 😊
Your video is helping me for NanoTech in Canada thank you😃
Amazing video, easily sb samgh aa gya .
What an amazing talent is this. Simplified the topic so that it can reach anyone who listens to this, Thanks a lot, sir. This is really a divine service
Nice explanation sir 🙏
Sir, first of all watch your all lectures which were nice I may be Wrong but at time 20:00 the white colour in TEM is represent absence of atoms and black colour represent presence of atoms of specific shapes as we did TEM in our Lab.
No I think you are wrong. Black reprenets nothing and gray or gray white colour represents the electron beam from the sample are more passed.. Overall the image is of gray white scale.. I might be wrong😊
Superb explanation sir..... thanks for this lacture 😊
LOVED THE ENERGY SIR....!
Bro 🥺 Etna acche se samjya hai na ki aakho me aashu aa gye thanks so much bro❤❤❤
Kya gazab ka pdhate ho sir😍😍
One of the best 😊
Thankyou so much sir I'm student of msc. Zoology...it helped me a lot...easy kr diya sir concept blkl...
I'm a botany student..& this topic is in our syllabus it really helps me a lot ...I understand everything..thanku soo much for making this veido sir 🙏
Thank you sir...very detailed explained and easily explained. Thanks
Ye jo aapne baat boli dil se padha raha hun i am also a teacher main deakh shakta tha ki ye. Aat bilkul sach hai ki aap bahut dil se padha rahe the ki ye chij real life me kaise kaaam karti hai.
bahut acche se aap concept clear kr dete ho bhaiay , ek hi baar mein pura samaj aa jata h. thanks yo u bhaiya aapke wajah se mere mid mein acche marks aaye
When I was watching your vedios I remember those days when I used to study from physicswallah in class 12 ..your way of teaching is like him but I can't compare you with him he is the legend he is the bestesttt but I must say that I've seen many vedios of graduation topics till now but yours is the best literally I've never enjoyed studying graduation topic this much.. you are so confident and your way of teaching is awesome now I can suggest you as best teacher for graduation and post graduation physics 👍
great explanation sir..keep uploading such lectures🙏🙏
Yesss ye video Hume bht pasand aai aaur hamare lie bahotttt helpful bhi Rahi..Thankyou as always 💓💓☺️
After watching your 5 or 6 video I can't stop my hand to not to subscribe your channel ❤❤❤ very well explain action 🎉🎉🎉🎉
Thank you so much 😀
Aweosoms explain🎉
behtareen.. bahot dhanyawad ❤
Pw vibe..jee yaad aa gya
Sir bhuut bdiya pdaate ho
At last i found ur channel and ur way of teaching mind-blowing it helps me alott.... thank you sirr.. 💫🙏❣️
Good explanation sir 👍
Thanku so much sir vry helpful video 😊
बेहतरीन बहुत बहुत धन्यवाद Sir😮😮😮
Great work❤
Vese m comment nhi krti hu but aako dekh k alakh pandey sir ki yaad aa gyi Aaj Mera exam h and this video is very useful for me thank you so much apne bht clear explanation diya ❤❤❤keep it up
Thanks sir very useful in phd coursework.
👏👏👏no words sir
Dhanyawad sir ji
Great 👍💯😃😸👌
Really impressive lecture 👏🏻
Very good luck for your channel sir ❤
my nanophysics exam is tomorrow .thanks sir ...for all your videos
You are teaching absolutely grt..i am having my exam 2moro..i wish i could also study spm stem and afm on your channel
Spm and stem are available in single video in stem
Thanks sir for make phy very easy to understand
very well explained
Thanks for liking
best lecture!
Wah sir Maja aa gaya
watch from 17:00 and then watch 0n 29:30 ,its confusing between dark and bright
I wish you were my class teacher❤
I think transmitted electrons are passing and reach the detector instead of secondary electrons. Secondary electrons are detected in SEM
Sir isme phase contrast transfer function
And HRTEM pr bhi video bna dijiye please
Sir please koi topics suggest kar dijiye cuet pg nanotechnology ke exam hai 12 ko ..... thankyou for great lectures.
Superb
Thank you sir
Thank so much
Sir
1)why sample used after condenser and before objective lens ? Pls 🙏🏼🙏🏼🙏🏼 tell me
Sir chemistry ka naochemisrtry bhi cover kr skte h kya.... chemistry and physics ka same topic h kya?
Please send syllabus of nano chemistry to my gmail id
Thank you
🎉
sir plz a upload a video of MRI scan .. plz plz plz sir
❤
Haa bhai full feel aagaya 😂
Sir mujhe fluorescence microscopy video chahiye
Tho sir... Cell ka internal structure ka kaise pta chalega??
Wow
❤❤❤❤
Sir aaj spm and stem and AFM provide Kara dijye please humble request my favorite sir
Kon si light objective me image banae gi ?
By doing metal layering we can only see the shape of the boundary of cell, we cannot know about the things inside
Yea true.
I can't understand
1) how electrons pass solid lenses?
2)if secondary electron are replaced primery electron so how can generate dark image?
3) if we don't know the shape of any cell so how can made solid metal frame for it ?
Exactly same query with me. Sir pls solve the query
Exactly, i have the same confusion
@bhagwatprasadbaghel7185
1) Rather than having a glass lens focusing the light (as in the case of light microscopes), the TEM employs an electromagnetic lens which focuses the electrons into a very fine beam, which means those are not solid lenses they are circular electromagnetic lenses.
2)When the beams strike the phosphor image screen light is generated, which causes
the image to be visible to the operator. The area through which very few electrons are
transmitted look darker (they are thicker or denser). The lighter areas of the image
represent those areas of the sample through which more electrons were transmitted
(they are thinner or less dense). In the video at first he explained wrongly and later he explained correctly.
3) Specimens must be very thin so that electrons are able to pass through the tissue. This may be done by cutting very thin slices of a specimen’s tissue using an ultramicrotome. The tissue must first be put in a chemical solution to preserve the cell structure. The tissue must also be completely dehydrated (all water removed). Once preserved and dehydrated, tissue samples are placed in hard, clean plastic. The plastic supports the tissue while it is being thinly cut with the ultramicrotome(you can google the image of this device).
After sections are cut and mounted on grids, (tiny circular disks with openings,) a solution of lead is used to stain the tissue. The lead provides contrast to the tissue by staining certain cell parts. When placed in the electron microscope, the electrons are scattered by the lead. They do not penetrate the tissue or hit the fluorescent screen, leaving those areas dark.
We don't find the shape of the cell to prepare a metal frame for it, In the video solid metal frame means the metal absorbed by some parts of cell.
Tunneling electron microscope?
I already made a video on Tunneling Electron Microscope
Please check channel " Physics Of Nanomaterials " Playlist for video on this topic.
Where are applications and disadvantages
Iski working ka diagram aapne bta diya likhna kaise hai ye nhi btayaa
Koi feel nae are aray agar cell ki layering kr de it's means ap ko pehly hi us ka structure pata chal gaya 😊
ese to metal s external structure pta chla internal kese pta chlega ?
Sir ftir and uv visible
Sir plz can you upload vedio of atomic force microscope ??
Ok
Metal ki plating krte time hme to phle se pta hoga na ki hmne kis shape ki plating ki h
Plating krne ke baad electron kyu pass krbane😂😂
I'm an chemical Engineering student my exam is tomorrow..fati padi hai
Sir notes milte to or achha hota
Ok
Ye sir kisse baat kr ke poochte rhte hai
Chota alakh Pandey (physics wallah ) 😊
Start of lecture is boring
😊