Great video. 😮 Sir, what could have gone wrong if blank (control) has a higher reading (O.D.) value than the standard but they have same color visually.
Varies depending on the kit you buy. Should be the same volume of Bradford reagent in every tube, because the variable you are changing is protein concentration
Thanks for the feedback. Unfortunately slow videos have low watch time and the algorithm downvotes them on RUclips. Will try to make every video better
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Great video. 😮 Sir, what could have gone wrong if blank (control) has a higher reading (O.D.) value than the standard but they have same color visually.
A very helpful explanation, would you be able to talk about the Modified Lowry Method Assay and procedure?
Very clear. Thanks.
Great video! Really helped me with my lab report : )
Great to hear - thanks for watching
How do I decide the concentration of protein I should load per well when running my western blot? Help!
How much of Bradford reagent should we add?
Varies depending on the kit you buy. Should be the same volume of Bradford reagent in every tube, because the variable you are changing is protein concentration
Sorry ! Is there some software or algorithm for drawing the standard curve ?
Nothing fancy, just Microsoft excel or you can draw it by hand.
@@BioLabCollective Thanks very much
My concentration is 0.3952mg/ml how can its calculate its volume to load maximum 50ug of protein per well?
We go into this topic here: Laboratory Solutions and Dilutions
ruclips.net/video/t2eC_hvaIBc/видео.html
concentration = mass / volume
good vid but you did too much cutting while edditing, it seems too fast to understand
Thanks for the feedback. Unfortunately slow videos have low watch time and the algorithm downvotes them on RUclips. Will try to make every video better
I like your voice 😅
Thanks for watching! Every bit counts for small channels