This is so wonderful! Save my time of struggling in grad school!Thank you so much!!! :)
Thanks for the video. If using gene-specific primers for quantification/detection, is it as necessary to bind towards the 3' end?
hi there, could you suggest which variant to pick during primers designing?
That depends on your research interest. Are you interested in a specific variant? Otherwise you can also design primers that amplify all variants
Can you make video about plasmid construction
How can I design primers that cover several transcripts of one gene so I do not have to make a primer pair for each variant?
same question
thank you :)
PCR is like Goldilocks, not too long not too short just right.
Actually, melting temperature is when 50% of a primer molecules is annealed to the target sequence, not just a half of it sequence!
Hi Vitaly. You are completely right. I realised this after I uploaded the video so it is not easy for me to edit the video to correct this.
i intend to employ sybr green rt-pcr, so primer set that could "potentially"/not 100% complementarity binding to other unspecific region should be avoided? assume my gDNA is absence.
Yes, that is correct :) try and avoid any off targets
This is so wonderful! Save my time of struggling in grad school!
Thank you so much!!! :)
Thanks for the video. If using gene-specific primers for quantification/detection, is it as necessary to bind towards the 3' end?
hi there, could you suggest which variant to pick during primers designing?
That depends on your research interest. Are you interested in a specific variant? Otherwise you can also design primers that amplify all variants
Can you make video about plasmid construction
How can I design primers that cover several transcripts of one gene so I do not have to make a primer pair for each variant?
same question
thank you :)
PCR is like Goldilocks, not too long not too short just right.
Actually, melting temperature is when 50% of a primer molecules is annealed to the target sequence, not just a half of it sequence!
Hi Vitaly. You are completely right. I realised this after I uploaded the video so it is not easy for me to edit the video to correct this.
i intend to employ sybr green rt-pcr, so primer set that could "potentially"/not 100% complementarity binding to other unspecific region should be avoided? assume my gDNA is absence.
Yes, that is correct :) try and avoid any off targets