Yes, it depends on what you are doing. The example in the video is for a full gene knockout / replacement with a knamycin resistance gene. If the knockout is successful, mutants can be selected on media supplemented with kanamycin. Other methods may rely on specific gene disruption by inserting sequences or deleting parts of coding sequences. Best wishes.
In the video, the kanamycin resistance gene is expressed using a promoter already in the genome at the knockout site. You could probably use an existing promoter or insert one.
Great video
Well described video
Great! Glad you thought it was well described. Best wishes!
Do we need to add full gene sequence of kanamycin or portion of it
Yes, it depends on what you are doing. The example in the video is for a full gene knockout / replacement with a knamycin resistance gene. If the knockout is successful, mutants can be selected on media supplemented with kanamycin. Other methods may rely on specific gene disruption by inserting sequences or deleting parts of coding sequences. Best wishes.
@genomeprojects and is it necessary to put promoter also
In the video, the kanamycin resistance gene is expressed using a promoter already in the genome at the knockout site. You could probably use an existing promoter or insert one.