Canu: Long Read Genome Assembly Tool

In this video, we will explore Canu, used for assembling long read sequencing data. We will perform assembly taking an example data followed by checking assembly statistics using Quast.
Link for the example data used: drive.google.c...

[@vaishnavigangadhar]

Very helpful video! Thank you!
Can you give a lesson on Whole Genome Sequencing

[@bioinfquests]

Thank you Vaishnavi. Yes am planning to post few videos on NGS series. Pls subscribe to the channel to get notifications.

[@percytullumevergara2823]

Excelent tutorial

[@kristintalia6978]

for the make -j, what does that mean? after that what to do to process your fastq.gz data? thank you sir

[@bioinfquests]

Number of threads in your system. If using linux nproc command will tell how many cpus. Accordingly you can specify while compilation.

[@marimbadearcomasaya3219]

Very helpfull video!. I have a question, why do not you use a reference genome to assembly?

[@bioinfquests]

See there are 2 possibilities
1. You have reference genome already known e.g. human. 2. You do not have reference genome e.g. a new species is identified and you plan to sequence them. In former case you can use already known genome reference information to guide the assembly process. In second case, you are newly doing assembly to generate reference genome. In first case at least you can compare the assembly with reference to infer how close your sample is with the reference one while in second case you have to trust or validate the assembly based on assembly statistics or some other literature evidence or prior experimental evidence.