Luciferase Reporter Assay | Reporter Gene Assay |

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  • Опубликовано: 2 окт 2024
  • Luciferases are a class of oxidative enzymes that occur in several species
    The enzyme enables the organisms that posess it to “bioluminesce,” or we can say it makes them to emit light.
    One of the most famous of these enzymes is the firefly luciferase.
    Fireflies have the ability to emit light through a chemical reaction in which luciferin is converted to oxyluciferin with the help of luciferase enzyme.
    Now the reaction catalyzed by luciferase is highly energetically efficient, that means all the energy supplied into the reaction is rapidly converted to light.
    Becuase of this, the enzyme is extremely sensitive, which makes it a great candidate for a reporter assay!
    Now lets know What is a Luciferase Reporter Assay?
    A luciferase reporter assay, is a kind of test , which determines whether a protein can activate... or repress the expression of the gene of interest , with the help of luciferase that is used as a reporter protein.

Комментарии • 36

  • @sheriguzman8586
    @sheriguzman8586 3 года назад +12

    Is this the same thing in these covid vaccines?

    • @BMHlearning
      @BMHlearning  3 года назад +2

      No covid vaccine is different...
      The video ia about a molecular biology tool used to trace the protein of interest.

    • @sheriguzman8586
      @sheriguzman8586 3 года назад +5

      @@BMHlearning I thought that's why the used the luciferase, to track the protein of the virus. Lol but I have no clue about this type of stuff. Just sparks my interest, so please forgive my ignorance.

    • @BMHlearning
      @BMHlearning  3 года назад +2

      Thats ok... Asking question increases knowledge & confidence.

    • @tlit3284
      @tlit3284 3 года назад +13

      It is in vaccine. Devil copy’s everything from God . Just look at the name Lucifer

    • @tlit3284
      @tlit3284 3 года назад +4

      As a name for the Devil in Christian theology, the more common meaning in English, "Lucifer" is the rendering of the Hebrew word הֵילֵל‎ (transliteration: hêylêl; pronunciation: hay-lale)[3] in Isaiah[4] given in the King James Version of the Bible. The translators of this version took the word from the Latin Vulgate,[5] which translated הֵילֵל by the Latin word lucifer (uncapitalized),[6][7] meaning "the morning star, the planet Venus", or, as an adjective, "light-bringing".[8]
      As a name for the planet in its morning aspect, "Lucifer" (Light-Bringer) is a proper name and is capitalized in English. In Greco-Roman civilization, it was often personified and considered a god[9] and in some versions considered a son of Aurora (the Dawn).[10] A similar name used by the Roman poet Catullus for the planet in its evening aspect is "Noctifer" (Night-Bringer).[11]

  • @bquick94
    @bquick94 3 года назад +7

    Black lights are being installed in street lamps across the country. Why exactly?

    • @seamanlab9665
      @seamanlab9665 2 года назад +3

      Blacklights don't detect luciferase. Luciferase creates light and therefore is "luminescent". Black lights allow you to see things that fluoresce. Luminescence is completely different than fluorescence.

    • @wildnature8773
      @wildnature8773 2 года назад

      To damage our bodies

  • @arijitghosh8599
    @arijitghosh8599 3 года назад +1

    If I want to understand the source of a protein from a specific tissue after stimulation with a chemical, can this technique be used?

  • @canalgilmardiasfreire306
    @canalgilmardiasfreire306 2 года назад +1

    Muito esclarecedor. Ótimo vídeo, obrigado!

  • @hatawa6135
    @hatawa6135 Год назад

    great video, but your voice is a little quiet and hard to hear

  • @atherulislam7367
    @atherulislam7367 Год назад

    Not convincing

  • @sonalsevda4072
    @sonalsevda4072 11 месяцев назад

    explaination👏

  • @R33VO
    @R33VO 2 года назад

    Why is this in the COVID vaccine

    • @BMHlearning
      @BMHlearning  2 года назад

      Its for research purposes...follow the link
      www.promegaconnections.com/luminescent-reporter-viruses/

  • @stephaniewilliams9469
    @stephaniewilliams9469 2 года назад

    What DingALing named it “Lucifer ase?”

    • @seamanlab9665
      @seamanlab9665 2 года назад

      Luciferase is from the Latin for "to make light" ("lux" = light; "fer" = to make). It is an enzyme derived from fireflies (sometimes called lightning bugs) that literally "makes light". To answer your question, the DingALing who actually named it was a French chemist, Raphaël Dubois, working in the 1880s.

  • @sandile84
    @sandile84 3 года назад +3

    Can this technology be used for ulterior motives if in the wrong hands? For example, for governments to check on people's skin to see if people have indeed been vaccinated & are not lying?

    • @BMHlearning
      @BMHlearning  3 года назад +3

      In my opinion vaccination is for one's own benifit, and todays generation is educated and aware about vaccination.
      Even if we agree with ur statement, then we need to think about the huge money expenditure ( technology + tracking vaccine in each individual )

    • @bquick94
      @bquick94 3 года назад +2

      That’s exactly why there have been black light street lights installed across the country. See it to believe it.

    • @Amarya_Qadash
      @Amarya_Qadash 2 года назад +2

      The Beast System and their mark is here don’t get any type of 💉, cv-tests, pills or free food/water.

    • @kimberlywood7783
      @kimberlywood7783 2 года назад +3

      @@BMHlearning you're limiting the thoughts to people with money restrictions. Government literally can just print more money.

    • @Lyns777
      @Lyns777 Год назад

      @@kimberlywood7783 or create digital money!
      Which is whats going to happen next 🤔