Towards a mechanistic understanding of motile and primary cilia with CLEM and cryo-electron...

Presented By:
Gaia Pigino
Speaker Biography:
Gaia Pigino is the Associate Head of the Centre for Structural Biology of the Human Technopole in Milan, Italy. Her team operates right at the interface between structural and molecular cell biology, using the latest methodologies from both fields to study the molecular machines required for the assembly and function of motile and primary cilia. She received her PhD in Evolutionary Biology from the University of Siena, Italy in 2007 for her studies of bio-indicators for contaminated soils. EM quickly became central in her research. After a short postdoc in the EM Lab of the University of Siena (2007-2009), she moved to Switzerland, where she worked at ETH Zurich and the Paul Scherer Institute to investigate the structure of ciliary components using cryo-EM (2009-2012). In 2012, Dr. Pigino became a research group leader at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany, from where she recently moved to the Human Technopole in Milan.
Webinar:
Towards a mechanistic understanding of motile and primary cilia with CLEM and cryo-electron tomography and expansion microscopy
Webinar Abstract:
In this talk I will show how the power of modern electron microscopy (EM) techniques, coupled with light microscopy and expansion methods, can open a window to the molecular dynamics of cells, enabling us to discover details about cellular processes at unprecedented level of detail. More concretely, I will show how we have used time resolved correlative light and electron microscopy (CLEM), cryo-FIB-SEM/cryo-electron tomography (cryo-ET) and expansion microscopy (U-ExM) paired with confocal fluorescence microscopy, TIRF microscopy and mechanical manipulations of cells to study a number of essential molecular processes in motile and primary cilia, i.e. conserved organelles that are fundamental for most eukaryotic cells. While our own work focuses on the inner workings of cilia, many open questions in cell and molecular biology can be addressed by combining modern imaging methods and methods from cell biology in suitable ways.
Earn PACE Credits:
1. Make sure you're a registered member of LabRoots (www.labroots.c...)
2. Watch the webinar on the LabRoots Website (www.labroots.c...)
3. Click Here to get your PACE credits (Expiration date - NOV 16, 2023): (www.labroots.c...)
LabRoots on Social:
Facebook: / labrootsinc
Twitter: / labroots
LinkedIn: / labroots
Instagram: / labrootsinc
Pinterest: / labroots
SnapChat: labroots_inc