LC-MS/MS Fundamentals

LC-MS/MS is a powerful quantitative and qualitative tool that has many advantages over other analytical techniques in terms of sensitivity, specificity, and versatility.
The presentation shows:
The fundamentals and theory of mass spectrometry (MS) including how to improve separation with liquid chromatography (LC), as well as key terms and techniques.
Need-to-know information about the fundamentals and terminology for accurate mass technology.

[@Harshavardhan-xy6xo]

Impressive … perfectly covered all the points.. Thanks for the detailed explanation

[@AmitKumar-ee4sc]

Nicely presented😀

[@russellgu8y2447]

Very helpful thankyou

[@sarvanks7711]

Thanks. Can we able to predict the no of isomers present in the mixtures using the LCMS/MS

[@Ccccccccccsssssssssss]

It depends. Some isomers will react differently with HPLC column, and therefore will have different retention times. For example, a branched chain isomer will typically have less interaction with the packing of the column, and will elute early, while a straight chain isomer will tend to have more interaction with the column and will elute later. In cases like this, you will see multiple peaks in a chromatogram, which each represent a different isomer. However, this is very dependent on the type of isomer (for example stereoisomers, or large molecules with isomers that are very similar in structure will be difficult to separate) and also dependent on the relationship between the compound and the column, and the mechanism of interaction between the two. I hope this is helpful.

[@sarvanks7711]

@@Ccccccccccsssssssssss Thankk you sir

[@abusupyanadiyev7067]

Good

[@hermon810]

Voice quality is poor. Please try to address this for future presentations.